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Anti-allergic effect of Lactobacillus rhamnosus IDCC 3201 isolated from breast milk-fed Korean infant

한국인 모유영양아의 분변에서 분리한 Lactobacillus rhamnosus IDCC 3201의 항 알레르기 효과

  • Lee, Seung-Hun (Bioprocess Engineering Team, Research Laboratories, ILDONG Pharmaceutical Co., Ltd.) ;
  • Kang, Jae-Hoon (Bioprocess Engineering Team, Research Laboratories, ILDONG Pharmaceutical Co., Ltd.) ;
  • Kang, Dae-Jung (Bioprocess Engineering Team, Research Laboratories, ILDONG Pharmaceutical Co., Ltd.)
  • 이승훈 (일동제약(주) 중앙연구소) ;
  • 강재훈 (일동제약(주) 중앙연구소) ;
  • 강대중 (일동제약(주) 중앙연구소)
  • Received : 2016.03.03
  • Accepted : 2016.03.16
  • Published : 2016.03.31

Abstract

We investigated 23 lactic acid bacteria isolated from Korean breast milk-fed infant in order to select strains which show superior anti-allergic effect. The candidates were cultivated and then we obtained dried powders of tyndallized cells and supernatant concentrate separately. Screening was carried out with down-regulation of interleukin (IL) 4 and up-regulation of IFN-${\gamma}$ in mouse splenocytes. As a result of the screening, we selected Lactobacillus rhamnosus IDCC 3201 (RH3201) for oral feeding to ovalbumin-sensitized BALB/c mice. Oral administration of RH3201 as dead cell bodies and supernatant concentrate suppressed hyper-production of serum immunoglobulin (Ig) E levels compared to vehicle group. Such anti-allergic effects were achieved by improvement of the balance between cytokines produced from type-1 helper T (Th1) and type-2 helper T (Th2) lymphocytes. Therefore, RH3201 has potential to improve atopic symptoms by immunomodulatory effect.

Keywords

Lactobacillus rhamnosus IDCC 3201;immunomodulation;tyndallization;immunoglobulin E

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Cited by

  1. Therapeutic effect of tyndallizedLactobacillus rhamnosusIDCC 3201 on atopic dermatitis mediated by down-regulation of immunoglobulin E in NC/Nga mice vol.60, pp.7, 2016, https://doi.org/10.1111/1348-0421.12390
  2. Development of Tyndallized Lactobacillus rhamnosus IDCC 3201 with Immunomodulation: Optimization, Validation, and in vitro Evaluation vol.32, pp.4, 2017, https://doi.org/10.7841/ksbbj.2017.32.4.271