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A unique thioredoxin reductase plays defensive roles against oxidative, nitrosative and nutritional stresses in Schizosaccharomyces pombe

Schizosaccharomyces pombe의 유일한 치오레독신 환원효소의 산화적, 일산화질소 및 영양 스트레스에 대한 방어적 역할

  • Ji, Dam-Jung (Department of Biochemistry, College of Natural Sciences, Kangwon National University) ;
  • Lim, Chang-Jin (Department of Biochemistry, College of Natural Sciences, Kangwon National University) ;
  • Kim, Kyunghoon (Department of Biological Sciences, College of Natural Sciences, Kangwon National University)
  • 지담정 (강원대학교 자연과학대학 생화학과) ;
  • 임창진 (강원대학교 자연과학대학 생화학과) ;
  • 김경훈 (강원대학교 자연과학대학 생명과학과)
  • Received : 2016.02.03
  • Accepted : 2016.03.16
  • Published : 2016.03.31

Abstract

A unique Schizosaccharomyces pombe $TrxR^+$ gene encoding thioredoxin reductase (TrxR) was found to be positively regulated by stress-inducing agents through the stress-responsive transcription factor Pap1. In the present study, the protective roles of S. pombe TrxR were evaluated using the TrxR-overexpressing recombinant plasmid pHSM10. In the presence of hydrogen peroxide ($H_2O_2$) and superoxide anion-generating menadione (MD), S. pombe TrxR increased cellular growth and the total glutathione (GSH) level, while it reduced levels of intracellular reactive oxygen species (ROS). The nitric oxide (NO) levels of the TrxR-overexpressing cells, in the presence of $H_2O_2$ and MD, were maintained to be similar to those of the corresponding non-treated cells. Although S. pombe TrxR was able to scavenge NO generated by sodium nitroprusside (SNP), it had no significant modulating effects on cellular growth, ROS levels, or the total GSH level of SNP-exposed yeast cells, compared with the differences in those of the two non-treated cell cultures. TrxR increased the cellular growth and total GSH level, which were diminished by nitrogen starvation. It also scavenged ROS and NO produced during nitrogen starvation. Taken together, the S. pombe TrxR protects against oxidative, nitrosative, and nutritional stresses.

Keywords

Schizosaccharomyces pombe;glutathione;hydrogen peroxide;nitric oxide;nitrogen starvation;reactive oxygen species;superoxide anion

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