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Anti-inflammatory Effect of Lactuca sativa L. Extract in Human Umbilical Vein Endothelial Cells and Improvement of Lipid Levels in Mice Fed a High-fat Diet

상추 추출물(Lactuca sativa L.)의 혈관내피세포에서 항염증 작용과 고지방 식이 생쥐에서 혈중 지질농도 개선에 미치는 영향

  • Hwang-Bo, Jeon (Dept. of Genetic Engineering and Graduate School of Biotechnology, Kyung Hee University) ;
  • Jang, Kyung Ok (Dept. of Genetic Engineering and Graduate School of Biotechnology, Kyung Hee University) ;
  • Chung, Hayoung (Cosmogen Co. Ltd.) ;
  • Park, Jong-Hwa (Dept. of Genetic Engineering and Graduate School of Biotechnology, Kyung Hee University) ;
  • Lee, Tae Hoon (Graduate School of East-West Medicinal Science Kyung Hee University) ;
  • Kim, Jiyoung (Cosmogen Co. Ltd.) ;
  • Chung, In Sik (Dept. of Genetic Engineering and Graduate School of Biotechnology, Kyung Hee University)
  • 황보전 (경희대학교 생명공학원 유전공학과) ;
  • 장경옥 (경희대학교 생명공학원 유전공학과) ;
  • 정하영 ((주)코스모젠) ;
  • 박종화 (경희대학교 생명공학원 유전공학과) ;
  • 이태훈 (경희대학교 동서의학대학원) ;
  • 김지영 ((주)코스모젠) ;
  • 정인식 (경희대학교 생명공학원 유전공학과)
  • Received : 2016.10.18
  • Accepted : 2016.12.13
  • Published : 2016.12.31

Abstract

The objective of this study was to investigate the effects of a lettuce (Lactuca sativa L.) extract on the inflammation of human umbilical vein endothelial cell (HUVEC) and blood lipid improvement in hypercholesterolemic mice fed a high cholesterol diet. The lettuce extract (100% ethanol extract) inhibited the expression of intercellular adhesion molecule-1 and vascular cell adhesion molecule-1 in HUVEC treated with tumor necrosis factor-${\alpha}$ (TNF-${\alpha}$). The lettuce extract suppressed the adhesion of THP-1 to TNF-${\alpha}$-treated HUVEC. The lettuce extract decreased the TNF-${\alpha}$-stimulated production of proinflammatory cytokine interleukin-6, interleukin-8 and chemokine monocyte chemotactic protein 1. In hypercholesterolemic mice, the lettuce extract reduced serum total cholesterol, triglyceride, and low-density lipoprotein-cholesterol level, while the lettuce extract elevated high-density lipoprotein-cholesterol level, resulting in the decrease of atherogenic index and cardiac risk factor level. These results suggested that lettuce extract can be an useful resource to show an anti-inflammatory effect and improve lipid metabolism.

Keywords

lettuce extract;inflammation;hypercholesterolemia;lipid metabolism

Acknowledgement

Supported by : 중소기업청

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