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The Investigation for the Effects of Citric Acid on the Uranium Transfer into the Plants by the Pilot Scale Feasibility Test

파일럿 규모의 실증실험 사례를 통한 구연산의 우라늄 식물 전이 효과 규명

  • Han, Yikyeong (Department of Earth Environmental Sciences, Pukyong National University) ;
  • Lee, Minhee (Department of Earth Environmental Sciences, Pukyong National University)
  • 한이경 (부경대학교 지구환경과학과) ;
  • 이민희 (부경대학교 지구환경과학과)
  • Received : 2016.11.21
  • Accepted : 2016.12.21
  • Published : 2016.12.31

Abstract

The field feasibility tests for a phytoextraction process were performed to identify the effect of citric acid as a chelate on the uranium (U) transfer into the plant for the naturally U contaminated soil in Duckpyeongri, Korea. For the feasibility tests, lettuce and Chinese cabbage were cultivated for 49 days on four testing grounds ($1m{\times}1m{\times}0.5m$ in each) in 2016. The citric acid solution was added to two testing grounds (one for lettuce and the other for Chinese cabbage) increasing the U transfer in two crop plants and their results were compared to those without the citric acid solution. When without the citric acid solution, the U concentration of plant after the cultivation was low (< $45{\mu}g/kg$ for leaves and < $450{\mu}g/kg$ for roots). However, with the addition of 50 mM citric acid solution, the U concentration of lettuce leaves and roots increased by 24 times and 1.8 times, and the U concentration of Chinese cabbage leaves and roots increased by 86.7 times and 5.4 times. The absolute accumulated U amount (${\mu}g$) in lettuce and Chinese cabbage also increased by 8.7 times and 50 times, compared to those without citric acid solution. Less than 8% of the U amount of exchangeable/carbonate phases was removed by using the lettuce and Chinese cabbage when the citric acid solution was not applied. However 52% and 66% of the U amount in exchangeable/carbonate phases were removed by the lettuce and the Chinese cabbage when the citric acid solution was added. The effect of the citric acid on the U transfer capability into the plants was quantitatively investigated by the field feasibility test, suggesting that U existing as exchangeable/carbonate phase in soil can be successfully removed by the phytoextraction process using Chinese cabbage with citric acid.

Keywords

Acknowledgement

Supported by : 한국연구재단

References

  1. Antonkiewicz, J. and Jasiewicz, C., 2002, The use of plants accumulating heavy metals for detoxification of chemically polluted soils, J. Pol. Agri. Univ., 5, 121-143.
  2. Bennett F.A., Tyler E.K., Brooks R.R., Gregg, P.E.H., and Stewart, R.B., 1998, Fertilization of hyperaccumulators to enhance their potential for phytoremediation and phytomining, Plants that Hyperaccumulate Heavy Metals, Cambridge, CABI Publishing, 249-259 (Brooks, RR. ed).
  3. Chon, H.T. and Jung M.C., 1991, Dispersion of toxic elements in the area covered with uranium-bearing black shales in Korea, J. Korean Inst. Min. Geol., 24, 3, 245-260.
  4. Dhankeher, O.P., Li, Y., Rosen, B.P., Shi, J., Salt, D., Senecoff, J., Sashti, N., and Meagher, R.B., 2002, Engineering tolerance and hyperaccumulation of arsenic in plants by combining arsenate reductase and gamma-glutamylcysteine synthetase expression, Nature Biotechnol., 20, 1140-1145. https://doi.org/10.1038/nbt747
  5. Duquene, L., Vandenhove, H., Tack, F., Meers, E., Baeten, J., and Wannijn, J., 2009, Enhanced phytoextraction of uranium and selected heavy metals by Indian mustard and ryegrass using biodegradable soil amendments, Sci. Total Environ., 407, 1496-1505. https://doi.org/10.1016/j.scitotenv.2008.10.049
  6. Han, Y.K., Park, S.M., and Lee, M.H., 2014, Phytoextraction using citric acid for enhanced removal of uranium from soil, J. Geol. Soc. Korea, 50, 501-515.
  7. Huang, J.W., 1992, Aluminum effects on calcium fluxes at the root apex of aluminum-tolerant and aluminum-sensitive wheat cultivars, Plant Physiol., 98, 230-237. https://doi.org/10.1104/pp.98.1.230
  8. Huang, J.W. and Cunningham, S.D., 1996, Lead phytoextraction: Species variation in lead uptake and translocation, New Phytol., 134, 75-84. https://doi.org/10.1111/j.1469-8137.1996.tb01147.x
  9. Huang, J.W., Blaylock, M.J., Kapulnik, Y. and Ensley, B.D., 1998, Phytoremediation of uranium-contaminated soils: Role of organic acids in triggering uranium hyperaccumulation in plants, Environ. Sci. Technol., 32, 2004-2008. https://doi.org/10.1021/es971027u
  10. Jung, M.C., 1994, Sequential extraction of heavy metals in soils and A case study, Econ. Environ. Geol., 27, 5, 469-477.
  11. Katsoyiannis, I.A., Althoff, H.W., Bartel, H., and Jekel, M., 2006, The effect of groundwater composition on uranium(VI) sorption onto bacteriogenic iron oxides, Water Res., 40, 3646-3652. https://doi.org/10.1016/j.watres.2006.06.032
  12. Kim, J.S. and Chon, H.T., 1997, Dispersion and enrichment of potentially toxic elements in Chubu area covered with black shales and slates in Korea, Econ. Environ. Geol., 30, 2, 89-103.
  13. Kim, O.J., 1982, Geology and mineral resources of the Korea, University of Yonsei Press, 33-44.
  14. Kumar, N.P.B.A., 1995, Phytoextraction: The use of plants to remove heavy metals from soil, Environ. Sci. Technol, 29, 1232-1238. https://doi.org/10.1021/es00005a014
  15. Langmuir, D., 1997, Aqueous Environmental Geochemistry, Prentice-Hall, Inc. Upper Saddle River, NJ.
  16. Lasat, M.M., 1998, Phytoremediation of a radio cesium-contaminated soil: evaluation of cesium-137 bioaccumulation in the shoots of three plant species, J. Environ. Qual., 27, 165-169.
  17. Lee, C.H. and Kim, J.H., 1972, Explanatory Text of the Geological Map of Goe San Sheet(1:50,000), Geological Survey of Korea.
  18. Lee, D.S., 1986, Mineralogy of low-grade uranium ores in the black slate of the Ogcheon goup, Korea, J. Korean Inst. Min. Geol., 19, 133-146.
  19. Lee, J.S., Chon H.T., and Kim, K.W., 1997, Dispersion and migration of potentially toxic elements in the rock-soil-plant system from the boeun area by underlain by black shales, Korea, Econ. Environ. Geol., 30, 6, 589-601.
  20. Lee, M.S. and Chon, H.T., 1980, Geochemical correlations between uranium and other components in U-bearing formation of Okchon belt, J. Korean Inst. Min. Geol., 13, 4, 241-246.
  21. Lozano, J.C., Rodriguez, P.B., Tome, F.V., and Calvo, C.P., 2011, Enhancing uranium solubilization in soils by citrate, EDTA, and EDDS chelating amendments, J. Hazard. Mater., 198, 224-231. https://doi.org/10.1016/j.jhazmat.2011.10.026
  22. Macek, T., Mackova, M., and Kas, J., 2000, Exploitation of plants for the removal of organics in environmental remediation, Biotechnol. adv., 18, 23-34. https://doi.org/10.1016/S0734-9750(99)00034-8
  23. Madrid, F., Liphadzi, M.S., and Kirkham, M.B., 2003, Heavy metal displacement in chelate-irrigated soil during phytoremediation, J. Hydrol., 272, 107-119. https://doi.org/10.1016/S0022-1694(02)00258-5
  24. Mihalik, J., Henner, P., Frelon, S., Camilleri, V., and Fevrier, L., 2012, Citrate assisted phytoextraction of uranium by sunflowers: Study of fluxes in soils and plants and resulting intra-planta distribution of Fe and U, Environ. Exp. Bot., 77, 249-258. https://doi.org/10.1016/j.envexpbot.2011.11.024
  25. Ministry of environment (MOE). 2009, Soil pollution process test standard, Ministry of Environment Republic of Korea.
  26. NIAST, 2000, Methods of soil and plant analysis, National Institute of Agricultural Science and Technology, Suwon, Korea.
  27. Pabalan, R.T. and Turner, D.R., 1997, Uranium(6+) sorption on montmorillonite: experimental and surface complexation modeling study, Aquat. Geochem., 2, 203-226. https://doi.org/10.1007/BF00119855
  28. Phillips E.J.P., Landa E.R. and Lovley D.R., 1995, Remediation of uranium contaminated soils with bicarbonate extraction and microbial U(VI) reduction, J. Ind. Microbiol., 14, 203-207. https://doi.org/10.1007/BF01569928
  29. Reeves, R.D. and Baker, A.J.M., 2000, Metal-accumulating plants. In: Raskin, I. (Ed.), Phytoremediation of Toxic Metals: Using Plants to Clean Up the Environment, John Wiley & Sons, Incorporated, 193-229.
  30. Rural Development Administration (RDA), 1989, Report of the Farmland Cultivation, 395-410.
  31. Schmidt, U., 2003, Enhancing phytoextraction: the effect of chemical soil manipulation on mobility, plant accumulation, and leaching of heavy metals, J. Environ. Qual., 32, 1939-1954. https://doi.org/10.2134/jeq2003.1939
  32. Shin, D.B. and Kim, S.J., 2011, Geochemical characteristics of black slate and coaly slate from the uranium deposit in Deokpyeong area, Econ. Environ. Geol., 44, 5, 373-386. https://doi.org/10.9719/EEG.2011.44.5.373
  33. Souza M.P., Huang C.P., Chee N., and Terry N., 1999, Rhizosphere bacteria enhance the accumulation of selenium and mercury in wetland plants, Planta, 209, 259-263. https://doi.org/10.1007/s004250050630
  34. Tessier, A., Campbell, P., and Bisson, M., 1979, Sequential extraction proccedure for the speciation of particulate trace metals, Anal. Chem., 51, 844-851. https://doi.org/10.1021/ac50043a017
  35. Thayalakumaran, T., Vogeler, I., Scotter, D.R., Robinson, B.H., Clothier, B.E., and Green, S.R., 2000, Chelation therapy for dirty soils. 2000 Annual Meetings Abstracts. American Society of Agronomy, Crop Science Society of America, Soil Science Society of America, Madison, Wisconsin, 202.
  36. Turgut, C., Pepe, M.K., and Cutright, T.J., 2004, The effect of EDTA and citric acid on phytoremediation of Cd, Cr, and Ni from soil using helianthus annuus, Environ. Pollut., 131, 147-154. https://doi.org/10.1016/j.envpol.2004.01.017
  37. USEPA Method 1311, 1994, Toxicity cahracteristic leaching procedure, Test methods for evaluating soild waste, physical/chemical methods, SW-846, 3rd ed., Environmental Agency, Washington, DC.
  38. USEPA Method 1312, 1994, Synthetic precipitation leaching procedure, Test methods for evaluating soild waste, physical/chemical methods, SW-846, 3rd ed., Environmental Agency, Washington, DC.
  39. Waite, T.D., Davis, J.A., Payne, T.E., Waychunas, G.A., and Xu, N., 1994, Uranium(VI) adsorption to ferrihydrite: application of a surface complexation modell, Geochim. Cosmochim. Acta, 58, 5465-5478. https://doi.org/10.1016/0016-7037(94)90243-7
  40. Yang, X., Feng, Y., Hea, Z., and Stoffella, P.J., 2005, Molecular mechanisms of heavy metal hyperaccumulation and phytoremediation, J. Trace Elem. Med. Biol., 18, 339-353. https://doi.org/10.1016/j.jtemb.2005.02.007