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Antioxidant and Cellular Protective Effects against Oxidative Stress of Calendula officinalis Flowers Extracts in Human Skin Cells

사람피부세포에서 카렌둘라 꽃 추출물의 항산화 및 산화적 스트레스에 대한 세포보호효과

  • Xuan, Song Hua (Department of Fine Chemistry, Cosmetic R&D center, Cosmetic Industry Coupled Collaboration Center, Seoul National University of Science and Technology) ;
  • Kim, Ga Yoon (Hansung Science High School) ;
  • Yu, Ji Yeon (Hansung Science High School) ;
  • Kim, Jee Won (Hansung Science High School) ;
  • Yang, Ye Rim (Hansung Science High School) ;
  • Jeon, Young Hee (Hansung Science High School) ;
  • Jeong, Yoon Ju (Department of Fine Chemistry, Cosmetic R&D center, Cosmetic Industry Coupled Collaboration Center, Seoul National University of Science and Technology) ;
  • Kim, A Rang (Department of Fine Chemistry, Cosmetic R&D center, Cosmetic Industry Coupled Collaboration Center, Seoul National University of Science and Technology) ;
  • Park, Soo Nam (Department of Fine Chemistry, Cosmetic R&D center, Cosmetic Industry Coupled Collaboration Center, Seoul National University of Science and Technology)
  • 현송화 (서울과학기술대학교 정밀화학과 화장품종합기술연구소, 코스메틱 융.복합산업 지원 센터) ;
  • 김가윤 (한성과학고등학교) ;
  • 유지연 (한성과학고등학교) ;
  • 김지원 (한성과학고등학교) ;
  • 양예림 (한성과학고등학교) ;
  • 전영희 (한성과학고등학교) ;
  • 정윤주 (서울과학기술대학교 정밀화학과 화장품종합기술연구소, 코스메틱 융.복합산업 지원 센터) ;
  • 김아랑 (서울과학기술대학교 정밀화학과 화장품종합기술연구소, 코스메틱 융.복합산업 지원 센터) ;
  • 박수남 (서울과학기술대학교 정밀화학과 화장품종합기술연구소, 코스메틱 융.복합산업 지원 센터)
  • Received : 2016.10.19
  • Accepted : 2016.11.18
  • Published : 2016.12.10

Abstract

In this study, we investigated the total phenolic and flavonoid contents, antioxidant activity and cellular protective effects against oxidative stress on human skin cells in 50% ethanol extract and its fractions of Calendula officinalis (C. officinalis) flowers. We measured the antioxidant effects of 50% ethanol extract and its fractions of C. officinalis flowers on the free radical scavenging activity ($FSC_{50}$), the reactive oxygen species (ROS) scavenging activities ($OSC_{50}$) and the inhibition of intracellular ROS generation in human skin cells. These results showed that the antioxidant effect of the ethyl acetate and aglycone fraction was more than the 50% ethanol extract of C. officinalis flowers. We also investigated the cellular protective activity and the results showed that treatment of the ethyl acetate fraction ($0.05-3.13{\mu}g/mL$) protects human skin cells in a concentration-dependent manner when the skin cell damages were induced by treating them with $H_2O_2$. In addition, the aglycone fraction ($1.56-3.13{\mu}g/mL$) shows cellular protective effects on the UV-induced cell damages in a dose-dependent manner. These results suggest that the fractions of C. officinalis flowers can function as a natural antioxidant agent of cosmetics in human skin cells exposed to oxidative stress by ROS scavenging effects.

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