DOI QR코드

DOI QR Code

Lactic Acid Bacteria in Total Mixed Ration Silage Containing Soybean Curd Residue: Their Isolation, Identification and Ability to Inhibit Aerobic Deterioration

  • Li, Y. ;
  • Wang, F. ;
  • Nishino, N.
  • Received : 2015.03.27
  • Accepted : 2015.06.24
  • Published : 2016.04.01

Abstract

We investigated the effects of the predominant lactic acid bacteria (LAB) on the fermentation characteristics and aerobic stability of total mixed ration (TMR) silage containing soybean curd residue (SC-TMR silage). The SC-TMR materials were ensiled in laboratory silos for 14 or 56 days. LAB predominant in SC-TMR silage were identified (Exp. 1). Lactobacillus fermentum (L. fermentum) and Streptococcus bovis (S. bovis) were found in the untreated materials, Leuconostoc pseudomesenteroides (L. pseudomesenteroides) in 14-day silage and Lactobacillus plantarum (L. plantarum) in all silages. Pediococcus acidilactici (P. acidilactici), Lactobacillus paracasei (L. paracasei), and Lactobacillus brevis (L. brevis) formed more than 90% of the isolates in 56- day silage. Italian ryegrass and whole crop maize were inoculated with P. acidilactici and L. brevis isolates and the fermentation and aerobic stability determined (Exp. 2). Inoculation with P. acidilactici and L. brevis alone or combined improved the fermentation products in ryegrass silage and markedly enhanced its aerobic stability. In maize silage, P. acidilactici and L. brevis inoculation caused no changes and suppressed deterioration when combined with increases in acetic acid content. The results indicate that P. acidilactici and L. brevis may produce a synergistic effect to inhibit SC-TMR silage deterioration. Further studies are needed to identify the inhibitory substances, which may be useful for developing potential antifungal agents.

Keywords

Soybean Curd Residue-Containing TMR Silage;Aerobic Deterioration;Lactic Acid Bacteria;Synergistic Effect

References

  1. Adesogan, A. T., M. B. Salawu, A. B. Ross, D. R. Davies, and A. E. Brooks. 2003. Effect of Lactobacillus buchneri, Lactobacillus fermentum, Leuconostoc mesenteroides inoculants, or a chemical additive on the fermentation, aerobic stability, and nutritive value of crimped wheat grains. J. Dairy Sci. 86:1789-1796. https://doi.org/10.3168/jds.S0022-0302(03)73764-3
  2. Bolsen, K. K., A. Laytimi, R. A. Hart, L. Nuzback, and F. Niroomand. 1989. Effect of commercial inoculants on fermentation of 1987 and 1988 Kansas silage crops. In: Food for Thought. Pioneer Hi-bred Int. Inc., Des Moines, IA, USA.
  3. Cai, Y. 2001. The role of lactic acid bacteria in the preparation of high fermentation quality. Grassl. Sci. 47:527-533.
  4. Danner, H., M. Holzer, E. Mayrhuber, and R. Braun. 2003. Acetic acid increases stability of silage under aerobic conditions. Appl. Environ. Microbiol. 69:562-567. https://doi.org/10.1128/AEM.69.1.562-567.2003
  5. Driehuis, F. and S. J. W. H. Oude Elferink. 2000. The impact of the quality of silage on animal health and food safety: A review. Vet. Q. 22:212-216. https://doi.org/10.1080/01652176.2000.9695061
  6. Fitzsimons, A., F. Duffner, D. Curtin, G. Brophy, P. Okiely, and O. Connell. 1992. Assessment of Pediococcus acidilacticias a potential silage inoculant. Appl. Environ. Microbiol. 58:3047-3052.
  7. Muck, R. E. and L. Jr. Kung. 1997. Effects of silage additives on ensiling. In: Silage: Field to feed bunk. NRAES-99, Ithaca, NY, USA. pp. 187-199.
  8. Nishino, N., H. Harada, and E. Sakaguchi. 2003a. Evaluation of fermentation and aerobic stability of wet brewers' grains ensiled alone or in combination of various feeds as a total mixed ration. J. Sci. Food Agric. 83:557-563. https://doi.org/10.1002/jsfa.1395
  9. Nishino, N., M. Yoshida, H. Shiota, and E. Sakaguchi. 2003b. Accumulation of 1,2-propanediol and enhancement of aerobic stability in whole crop maize silage inoculated with Lactobacillus buchneri. J. Appl. Microbiol. 94:800-807. https://doi.org/10.1046/j.1365-2672.2003.01810.x
  10. Nishino, N., H. Wada, M. Yoshida, and H. Shiota. 2004. Microbial counts, fermentation products, and aerobic stability of whole crop corn and a total mixed ration ensiled with and without inoculation of Lactobacillus casei or Lactobacillus buchneri. J. Dairy Sci. 87:2563-2570. https://doi.org/10.3168/jds.S0022-0302(04)73381-0
  11. Nishino, N., H. Hattori, H. Wada, and E. Touno. 2007. Biogenic amine production in grass, maize and total mixed ration silages inoculated with Lactobacillus casei or Lactobacillus buchneri. J. Appl. Microbiol. 103:325-332. https://doi.org/10.1111/j.1365-2672.2006.03244.x
  12. Niwa, Y. 2001. Silage making and utilization of high-moisture byproducts. 4. Making silage from tofu cake and utilization. Grassl. Sci. 47:323-326.
  13. Rust, S. R., H. S. Kim, and G. L. Enders. 1989. Effects of a microbial inoculant on fermentation characteristics and nutritional value of corn silage. J. Prod. Agric. 2:235-241. https://doi.org/10.2134/jpa1989.0235
  14. Wang, F. and N. Nishino. 2008a. Ensiling of soybean curd residue and wet brewers grains with or without other feeds as a total mixed ration. J. Dairy Sci. 91:2380-2387. https://doi.org/10.3168/jds.2007-0821
  15. Wang, F. and N. Nishino. 2008b. Resistance to aerobic deterioration of total mixed ration silage: Effect of ration formulation, air infiltration and storage period on fermentation characteristics and aerobic stability. J. Sci. Food Agric. 88:133-140. https://doi.org/10.1002/jsfa.3057
  16. Wang, F. and N. Nishino. 2008c. Effect of aerobic exposure after silo opening on feed intake and digestibility of total mixed ration silage containing wet brewers grains or soybean curd residue. Grassl. Sci. 54:164-166. https://doi.org/10.1111/j.1744-697X.2008.00118.x
  17. Wang, F. and N. Nishino. 2009. Association of Lactobacillus buchneri with aerobic stability of total mixed ration containing wet brewers grains preserved as a silage. Anim. Feed Sci. Technol. 149:265-274. https://doi.org/10.1016/j.anifeedsci.2008.06.012
  18. Weinberg, Z. G., G. Ashbell, Y. Hen, and A. Azriel. 1993. The effect of applying lactic acid bacteria at ensiling on the aerobic stability of silages. J. Appl. Microbiol.75:512-518.

Cited by

  1. Effects of Organic Acids on Salmonella enteritidis Growth Inhibition and Ileum Surface Area in Laying Ducks Fed Anaerobically Fermented Feed vol.16, pp.3, 2017, https://doi.org/10.3923/ijps.2017.98.104
  2. Effect of microbial inoculants on fermentation quality and aerobic stability of sweet potato vine silage vol.31, pp.12, 2018, https://doi.org/10.5713/ajas.18.0264

Acknowledgement

Supported by : National Natural Science Foundation of China