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Anti-Stress Effect of Punica granatum L. Extract against Sleep Deprivation-Induced Impairment

석류 열수 추출물의 수면박탈을 유도한 Rat 모델에서의 항스트레스 효과

  • Na, Ju-Ryun (Bioresources and Technology) ;
  • Kim, Sunoh (Bioresources and Technology) ;
  • Jo, Ara (Jeonnam Institute of Natural Resources Research) ;
  • Bae, Donghyuck (Jeonnam Institute of Natural Resources Research) ;
  • Oh, Kyo-Nyeo (Jeonnam Institute of Natural Resources Research) ;
  • Kim, Yong Jae (Division of Food and Nutrition, Chonnam National University) ;
  • Lee, Yoo-Hyun (Department of Food Science and Nutrition, The University of Suwon) ;
  • Jun, Woojin (Division of Food and Nutrition, Chonnam National University)
  • Received : 2016.01.29
  • Accepted : 2016.09.29
  • Published : 2016.11.30

Abstract

The anti-stress effects of Punica granatum L. (family Lythraceae, PG) on $H_2O_2$/corticosterone (CORT)-induced stress in cells and sleep-deprived rats were investigated. The PG extract showed neuroprotective effects in SH-SY5Y cells against $H_2O_2$/CORT-induced stress. Sleep deprivation led to behavioral, hormonal, and biochemical alterations in the animal model. The effects of P. granatum on physiological, behavioral, and biochemical parameters aggravated by sleep deprivation were investigated. Sleep deprivation impaired physiological (survival, body weight, and drowsiness scores) and behavioral (rotarod, passive avoidance, hot hyperalgesia, and Y maze) parameters as well as biochemical factors (cortisol, serotonin, dopamine, testosterone, and growth factor I contents in serum). These parameters were significantly recovered by PG extract in a concentration-dependent manner. The PG extract also enhanced catalase, superoxide dismutase, and non-enzymatic antioxidative activities such as glutathione compared to sleep-deprived rats. On the basis of these results, our findings suggest that Punica granatum prevents impairment of body functions induced by sleep deprivation and related oxidative damage.

Acknowledgement

Supported by : 산업통상자원부

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