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Anti-oxidant and anti-inflammatory effects of Salix Koreensis Andersson in DC. leaf methanol extract in vitro models

  • Kim, Eun-Ji (Department of Food and Nutrition, Hoseo University) ;
  • Kim, Mi Hye (Department of Food and Nutrition, Hoseo University)
  • Received : 2016.09.12
  • Accepted : 2016.11.14
  • Published : 2016.11.30

Abstract

Oxidative rancidity in foods causes undesirable changes in nutritive value, aroma, flavor, and color. Salix Koreensis Andersson in DC. (SK) has anti-oxidative and anti-inflammatory effects and is traditionally used to treat neuralgia, edema, pain, and inflammatory diseases. However, the regulatory effects of SK on oxidative and inflammatory reactions have not been elucidated. In this context, we scientifically validated the anti-oxidative and anti-inflammatory activities of SK leaf (SKL). The methanol extract of SKL was evaluated for in vitro anti-oxidative activities. SKL showed increased superoxide dismutase (SOD)-like activity and 1, 1-diphenyl-2-picrylhydrazyl radical scavenging activity. The in vitro anti-oxidant and anti-inflammatory activities of SKL were also investigated in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages. LPS resulted in decreased SOD activities compared with the unstimulated cells, but SKL significantly increased SOD activities reduced by LPS. In addition, LPS-induced nitric oxide, tumor necrosis factor-${\alpha}$, and interleukin-6 productions were significantly and dose-dependently reduced by SKL in RAW264.7 macrophages without inducing cytotoxicity. In conclusion, these results indicate that SKL will be able to be effectively used as a food additive with anti-oxidative and anti-inflammatory effects.

Acknowledgement

Supported by : National Research Foundation of Korea

References

  1. Alam MS, Kaur G, Jabbar Z, Javed K, Athar M. Evaluation of antioxidant activity of Salix caprea flowers. Phytother Res. 2006;20:479-483. https://doi.org/10.1002/ptr.1882
  2. Balkwill F. Tumour necrosis factor and cancer. Nat Rev Cancer. 2009;9:361-371. https://doi.org/10.1038/nrc2628
  3. Basinska K, Marycz K, Sieszek A, Nicpon J. The production and distribution of IL-6 and TNF-${\alpha}$ in subcutaneous adipose tissue and their correlation with serum concentrations in Welsh ponies with equine metabolic syndrome. J Vet Sci. 2015;16:113-120. https://doi.org/10.4142/jvs.2015.16.1.113
  4. Benvenuti S , Pellati F, Melegari M, Bertelli D. Polyphenols, nthocyanins, Ascorbic Acid, and Radical Scavenging Activity of Rubus, Ribes, and Aronia. J. Food Science. 2004;69:164-169.
  5. Carocho M, Ferreira IC. A review on antioxidants, prooxidants and related controversy: Natural and synthetic compounds, screening and analysis methodologies and future perspectives. Food Chem Toxicol. 2013;51:15-25. https://doi.org/10.1016/j.fct.2012.09.021
  6. Clayson DB, Iverson F, Nera EA, and Lok E. The importance of cellular proliferation induced by BHA and BHT. Toxicol Ind Health. 1993;9:231-242. https://doi.org/10.1177/0748233793009001-217
  7. Davis WB. Determination of flavanones in citrus fruits. Anal Chem. 1947;19:476-478. https://doi.org/10.1021/ac60007a016
  8. Du Q, Jerz G, Shen L, Xiu L, Winterhalter P. Isolation and structure determination of a lignan from the bark of Salix alba. Nat Prod Res. 2007;21:451-454. https://doi.org/10.1080/14786410601083845
  9. Dua A, Singh A, Mahajan N. Antioxidants of clove (Syzygium aromaticum) prevent metal induced oxidative damage of biomolecules. Int Res J Pharm. 2015;6:273-278. https://doi.org/10.7897/2230-8407.06460
  10. Freischmidt A, Jurgenliemk G, Kraus B, Okpanyi SN, Muller J, Kelber O, Weiser D, Heilmann J. Contribution of flavonoids and catechol to the reduction of ICAM-1 expression in endothelial cells by a standardised Willow bark extract. Phytomedicine. 2012;19:245-252. https://doi.org/10.1016/j.phymed.2011.08.065
  11. Fu W, Chen J, Cai Y, Lei Y, Chen L, Pei L, Zhou D, Liang X, Ruan J. Antioxidant, free radical scavenging, anti-inflammatory and hepatoprotective potential of the extract from Parathelypteris nipponica (Franch. et Sav.) Ching. J Ethnopharmacol. 2010;130:521-528. https://doi.org/10.1016/j.jep.2010.05.039
  12. Han LK, Sumiyoshi M, Zhang J, Liu MX, Zhang XF, Zheng YN, Okuda H, Kimura Y. Anti-obesity action of Salix matsudana leaves (Part 1). Anti-obesity action by polyphenols of Salix matsudana in high fat-diet treated rodent animals. Phytother Res. 2003;17:1188-1194. https://doi.org/10.1002/ptr.1404
  13. Hocman G. Chemoprevention of cancer: Phenolic antioxidants (BHT, BHA). The Int J Biochem. 1988;20:639-651. https://doi.org/10.1016/0020-711X(88)90158-9
  14. Hosseini A, Herulf M, Ehren I. Measurement of nitric oxide may differentiate between inflammatory and non-inflammatory prostatitis. Scand J Urol Nephrol. 2006;40:125-130. https://doi.org/10.1080/00365590500193379
  15. Iqbal S, Bhanger MI, Anwer F. Antioxidant properties and components of bran extracts from selected wheat varieties commercially available in Pakistan. LWT-Food Sci Technol. 2007;40:361-367. https://doi.org/10.1016/j.lwt.2005.10.001
  16. Jeong HJ, Han NR, Kim KY, Choi IS, Kim HM. Gomisin A decreases the LPS-induced expression of iNOS and COX-2 and activation of RIP2/NF-${\kappa}$B in mouse peritoneal macrophages. Immunopharmacol Immunotoxicol. 2014;36:195-201. https://doi.org/10.3109/08923973.2014.909848
  17. Kandimalla R, Kalita S, Saikia B, Choudhury B, Singh YP, Kalita K, Dash S, Kotoky J. Antioxidant and Hepatoprotective Potentiality of Randia dumetorum Lam. Leaf and Bark via Inhibition of Oxidative Stress and Inflammatory Cytokines. Front Pharmacol. 2016;7:205.
  18. Kang MH, Park CG, Cha MS, Seong NS, Chung HK, Lee JB. Component characteristics of each extract prepared by different extract methods from by-products of Glycyrrhizia Uralensis. J Korean Soc Food Sci Nutr. 2001;30:138-142.
  19. Kennedy-Feitosa E, Okuro RT, Pinho Ribeiro V, Lanzetti M, Barroso MV, Zin WA, Porto LC, Brito-Gittirana L, Valenca SS. Eucalyptol attenuates cigarette smoke-induced acute lung inflammation and oxidative stress in the mouse. Pulm Pharmacol Ther. 2016;41:11-18. https://doi.org/10.1016/j.pupt.2016.09.004
  20. Kitanaka S. Chemical Compounds in Natural Medicines That Affect Macropharges and Adipocyte Cells. Yakugaku Zasshi. 2016;136:1195-1216. https://doi.org/10.1248/yakushi.16-00027
  21. Kong FL, Zhang MW, Kuang RB, Yu SJ, Chi JW, Wei ZC. Antioxidant activities of different fractions of polysaccharide purified from pulp tissue of litchi (Litchichinensis Sonn). Carbohydr Polym. 2010;81:612-616. https://doi.org/10.1016/j.carbpol.2010.03.021
  22. Lawrence T, Willoughby DA, Gilroy DW. Anti-inflammatory lipid mediators and insights into the resolution of inflammation. Nat Rev Immunol. 2002;2:787-795. https://doi.org/10.1038/nri915
  23. Li X, Liu Z, Zhang XF, Wang LJ, Zheng YN, Yuan CC, Sun GZ. Isolation and characterization of phenolic compounds from the leaves of Salix matsudana. Molecules. 2008;13:1530-1537. https://doi.org/10.3390/molecules13081530
  24. Liang D, Zhou Q, Gong W, Wang Y, Nie Z, He H, Li J, Wu J, Wu C, Zhang J. Studies on the antioxidant and hepatoprotective activities of polysaccharides from Talinum triangulare. J Ethnopharmacol. 2011;136:316-321. https://doi.org/10.1016/j.jep.2011.04.047
  25. Medzhitov R, Janeway CA Jr. Innate immunity: the virtues of a nonclonal system of recognition. Cell. 1997;91:295-298. https://doi.org/10.1016/S0092-8674(00)80412-2
  26. Nerin C, Tovar L, Salafranca J. Behaviour of a new antioxidant active film versus oxidizable model compounds. J Food Eng. 2008;84:313-320. https://doi.org/10.1016/j.jfoodeng.2007.05.027
  27. Pando MP, Verma IM. Signal-dependent and–independent degradation of free and NF-kappa B-bound IkappaBalpha. J Biol Chem. 2000;275:21278-21286. https://doi.org/10.1074/jbc.M002532200
  28. Park SN, Kim JY, Yang HJ, Lee KH, Jeon SM, Ahn YJ, Won BR. Antioxidative and Antiaging Effects of Jeju Native Plant Extracts (II). Journal of the Society of Cosmetic Scientists of Korea. 2007;33:165-173.
  29. Pawar N, Arora S, Bijoy RR, Wadhwa BK. The effects of Asparagus racemosus (Shatavari) extract on oxidative stability of ghee, in relation to natural and synthetic antioxidants. Int J Dairy Technol. 2012;65:293-299. https://doi.org/10.1111/j.1471-0307.2011.00816.x
  30. Ricciotti E, FitzGerald GA. Prostaglandins and inflammation. Arterioscler Thromb Vasc Biol. 2011;31:986-1000. https://doi.org/10.1161/ATVBAHA.110.207449
  31. Sarwar R, Farooq U, Khan A, Naz S, Khan S, Khan A, Rauf A, Bahadar H, Uddin R. Evaluation of Antioxidant, Free Radical Scavenging, and Antimicrobial Activity of Quercus incana Roxb. Front Pharmacol. 2015;6:277.
  32. Scalbert A, Manach C, Morand C, Remesy C, Jimenez L. Dietary polyphenols and the prevention of diseases. Crit Rev Food Sci Nutr. 2005;45:287-306. https://doi.org/10.1080/1040869059096
  33. Seczyk L, Swieca M, Dziki D, Anders A, Gawlik-Dziki U. Antioxidant, nutritional and functional characteristics of wheat bread enriched with ground flaxseed hulls. Food Chem. 2017;214:32-38. https://doi.org/10.1016/j.foodchem.2016.07.068
  34. Shpacovitch V, Feld M, Bunnett NW, Steinhoff M. Protease-activated receptors: novel PARtners in innate immunity. Trends Immunol. 2007;28:541-550. https://doi.org/10.1016/j.it.2007.09.001
  35. Sivam AS, Sun-Waterhouse D, Quek S, Perera CO. Properties of bread dough with added fiber polysaccharides and phenolic antioxidants: A review. J Food Sci. 2010;75:R163-174. https://doi.org/10.1111/j.1750-3841.2010.01815.x
  36. Siwach R, Tokas J, Seth R. Use of lycopene as a natural antioxidant in extending the shelf-life of anhydrous cow milk fat. Food Chem. 2016;199:541-546. https://doi.org/10.1016/j.foodchem.2015.12.009
  37. Soufli I, Toumi R, Rafa H, Touil-Boukoffa C. Overview of cytokines and nitric oxide involvement in immuno-pathogenesis of inflammatory bowel diseases. World J Gastrointest Pharmacol Ther. 2016;7:353-360. https://doi.org/10.4292/wjgpt.v7.i3.353
  38. Sultana S, Saleem M. Salix caprea inhibits skin carcinogenesis in murine skin: Inhibition of oxidative stress, Ornithine decarboxylase activity and DNA synthesis. J Ethnopharmacol. 2004;91:267-276. https://doi.org/10.1016/j.jep.2003.12.028
  39. Sun Z, Yan B, Yu WY, Yao X, Ma X, Sheng G, Ma Q. Vitexin attenuates acute doxorubicin cardiotoxicity in rats via the suppression of oxidative stress, inflammation and apoptosis and the activation of FOXO3a. Exp Ther Med. 2016;12:1879-1884. https://doi.org/10.3892/etm.2016.3518
  40. Tsuda T, Fukuya Y, Ohshima K, Yamamoto A, Kawakishi S, Osawa T. Antioxidative activity of tamarind extract prepared from the seed coat. Nippon Shokuhin Kag Kougaku Kaishi. 1995;42:430-435. https://doi.org/10.3136/nskkk.42.430