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Incidence of Active HCV infection amongst Blood Donors of Mardan District, Pakistan

  • Karim, Fawad (Department of Biotechnology, Bacha Khan University Charsadda) ;
  • Nasar, Abu (Department of Biotechnology, Bacha Khan University Charsadda) ;
  • Alam, Ibrar (Department of Biotechnology, Bacha Khan University Charsadda) ;
  • Alam, Iftikhar (Department of Human Nutrition and Dietetics (HND), Bacha Khan University Charsadda) ;
  • Hassan, Said (Center of Biotechnology and Microbiology, University of Peshawar) ;
  • Gul, Rahmat (Department of Human Nutrition and Dietetics (HND), Bacha Khan University Charsadda) ;
  • Ullah, Sana (Department of Animal Science Quaid-i-Azam University Islamabad) ;
  • Rizwan, Muhammad (Center of Biotechnology and Microbiology, University of Peshawar)
  • Published : 2016.02.05

Abstract

Hepatitis C is an ailment of liver caused by hepatitis C virus (HCV) infection. About 3% of the world population is infected by this virus. HCV infection is a leading reason for liver cirrhosis and therefore a major source of hepatocellular carcinoma. The study focused on the incidence of active HCV infection in blood donors of Mardan district of KPK, Pakistan. A total of 5318 blood donors were inspected for the presence of anti-HCV antibodies and HCV-RNA using ICT (immune-chromatographic test), ELISA and RT-PCR at Mardan Medical Complex (MMC), Mardan. Out of these, 157 (2.95%) were positive by ICT, 60 (1.12%) by ELISA and 56 (1.05%) for HCV-RNA. The frequency of active HCV infectivity amongst the blood donors from district Mardan, KPK Pakistan was 1.05 %. Application of strict measures during blood donor selection and use of proper screening assays such as ELISA in place of ICT devices can give a more accurate picture so that the incidence of this viral infection in HCV negative blood recipients can be reduced.

Keywords

HCV;ELISA;RT-PCR;viral infection rates

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