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Involvement of CELSR3 Hypermethylation in Primary Oral Squamous Cell Carcinoma

  • Khor, Goot Heah (Centre of Preclinical Science Studies, Faculty of Dentistry, Universiti Teknologi MARA, Sungai Buluh Campus) ;
  • Froemming, Gabrielle Ruth Anisah (Institute of Pathology, Laboratory and Forensic Medicine, Faculty of Medicine, Universiti Teknologi MARA, Sungai Buluh Campus) ;
  • Zain, Rosnah Binti (Oral Cancer Research and Coordinating Centre, Faculty of Dentistry, University of Malaya) ;
  • Abraham, Thomas Mannil (Tengku Ampuan Rahimah Hospital, Department of Oral and Maxillofacial Surgery, Ministry of Health Malaysia) ;
  • Lin, Thong Kwai (Institute of Biological Science, Faculty of Science, University of Malaya)
  • Published : 2016.02.05

Abstract

Background: Promoter hypermethylation is a frequent epigenetic mechanism for gene transcription repression in cancer and is one of the hallmarks of the disease. Cadherin EGF LAG seven-pass G-type receptor 3 (CELSR3) contributes to cell contact-mediated communication. Dysregulation of promoter methylation has been reported in various cancers. Objectives: The objectives of this study were to investigate the CELSR3 hypermethylation level in oral squamous cell carcinomas (OSCCs) using methylation-sensitive high-resolution melting analysis (MS-HRM) and to correlate CELSR3 methylation with patient demographic and clinicopathological parameters. Materials and Methods: Frozen tissue samples of healthy subjects' normal mucosa and OSCCs were examined with regard to their methylation levels of the CELSR3 gene using MS-HRM. Results: MS-HRM analysis revealed a high methylation level of CELSR3 in 86% of OSCC cases. Significant correlations were found between CELSR3 quantitative methylation levels with patient ethnicity (P=0.005), age (P=0.024) and pathological stages (P=0.004). A moderate positive correlation between CELSR3 and patient age was also evident (R=0.444, P=0.001). Conclusions: CELSR3 promoter hypermethylation may be an important mechanism involved in oral carcinogenesis. It may thus be used as a biomarker in OSCC prognostication.

Keywords

CELSR3;hypermethylation;oral squamous cell carcinoma;biomarker

Acknowledgement

Supported by : Universiti Teknologi MARA, Ministry of Higher Education

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