Anti-Proliferative Activity and Apoptosis Induction of an Ethanolic Extract of Boesenbergia pandurata (Roxb.) Schlecht. against HeLa and Vero Cell Lines

  • Listyawati, Shanti (Biotechnology, Universitas Gadjah Mada) ;
  • Sismindari, Sismindari (Faculty of Pharmacy, Universitas Gadjah Mada) ;
  • Mubarika, Sofia (Faculty of Medicine, Universitas Gadjah Mada) ;
  • Murti, Yosi Bayu (Faculty of Pharmacy, Universitas Gadjah Mada) ;
  • Ikawati, Muthi (Faculty of Pharmacy, Universitas Gadjah Mada)
  • Published : 2016.02.05


Rhizomes of Boesenbergia pandurata (Roxb.) Schlecht have been reported to contain active compounds with anticancer properties. This research was carried out to examine anti-proliferative and apoptotic induction against HeLa and Vero cells-line. Dried powder of B. pandurata rhizomes was extracted by a maceration method using 90% ethanol. Cytotoxic assays to determine $IC_{50}$ and anti-proliferative effects were carried out by MTT methods. Observation of apoptosis was achieved with double staining using acridine orange and ethidium bromide. The results showed that ethanolic extract of B. pandurata was more cytotoxic against HeLa cells ($IC_{50}$ of $60{\mu}g/mL$) than Vero cells ($IC_{50}$ of $125{\mu}g/mL$). The extract had higher anti-proliferative activity as well as apoptotic induction in HeLa than Vero cells. Therefore, it was concluded that the ethanolic extract of B. pandurata had anti-proliferative as well as apoptosis induction activity dependent on the cell type.


Boesenbergia pandurata;anti-proliferative activity;apoptosis;Hela cells;Vero cells


Grant : RISBIN IPTEKDOK 2011 Program

Supported by : Health Ministry


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