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Effect of LED Irradiation on Proliferation of Human Epidermal Keratinocyte for Convergence

LED조사가 인간 피부 각질세포의 증식에 미치는 융복합적인 영향

  • 박정숙 (남부대학교 간호학과) ;
  • 김미혜 (중부대학교 미용분장학과) ;
  • 이재혁 (남부대학교 한방제약개발학과)
  • Received : 2016.09.29
  • Accepted : 2016.11.20
  • Published : 2016.11.28

Abstract

The purpose of this study is to determine the effect of the light-emitting-diode (LED) to investigate proliferation of human epidermal keratinocyte and collagen, procollagen expression. In order to determine whether LED irradiation can safely be applied to human skin, the proliferative effects of LED irradiation were determined by MTS assay in Human Epidermal Keratinocytes. Wavelength of 470nm LED irradiation increased mRNA expression of collagen, procollagen without cytotoxity. Our results suggest that 470nm LED irradiation may have a proliferative effects and collagen synthesis property. In order to determine whether LED irradiation can safely be applied to human skin, the cytotoxic effects of LED irradiation were determined by MTS assay in Human Dermal Fibroblasts (HDF). As far as we know, this is the first report demonstrating in vitro collagen synthesis activity of 470nm LED irradiation and being a scientific basis for the cosmetic.

Keywords

LED(Light emitting diode);Human Epidermal Keratinocytes;Collagen;Procollagen;Light therapy;Convergence

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