DOI QR코드

DOI QR Code

Evaluation of DNA Damage Using Microwave Dielectric Absorption Spectroscopy

  • Received : 2015.07.17
  • Accepted : 2016.11.01
  • Published : 2016.12.31

Abstract

Background: Evaluation of deoxyribonucleic acid (DNA)-strand break is important to elucidate the biological effect of ionizing radiations. The conventional methods for DNA-strand break evaluation have been achieved by Agarose gel electrophoresis and others using an electrical property of DNAs. Such kinds of DNA-strand break evaluation systems can estimate DNA-strand break, according to a molecular weight of DNAs. However, the conventional method needs pretreatment of the sample and a relatively long period for analysis. They do not have enough sensitivity to detect the strand break products in the low-dose region. Materials and Methods: The sample is water, methanol and plasmid DNA solution. The plasmid DNA pUC118 was multiplied by using Escherichia coli JM109 competent cells. The resonance frequency and Q-value were measured by means of microwave dielectric absorption spectroscopy. When a sample is located at a center of the electric field, resonance curve of the frequency that existed as a standing wave is disturbed. As a result, the perturbation effect to perform a resonance with different frequency is adopted. Results and Discussion: The resonance frequency shifted to higher frequency with an increase in a concentration of methanol as the model of the biological material, and the Q-value decreased. The absorption peak in microwave power spectrum of the double-strand break plasmid DNA shifted from the non-damaged plasmid DNA. Moreover, the sharpness of absorption peak changed resulting in change in Q-value. We confirmed that a resonance frequency shifted to higher frequency with an increase in concentration of the plasmid DNA. Conclusion: We developed a new technique for an evaluation of DNA damage. In this paper, we report the evaluation method of DNA damage using microwave dielectric absorption spectroscopy.

Acknowledgement

Supported by : JSPS

References

  1. Yokota R, Nakajima S. Improved fluoroglass dosimeter as personnel monitoring dosimeter and microdosimeter. Health Phys. 1965;11(4):241-253. https://doi.org/10.1097/00004032-196504000-00001
  2. Bradshaw WW, Cadena DG Jr. The Use of Alanine as a solid dosimeter. Radiat. Res. 1962;17(1):11-21. https://doi.org/10.2307/3571206
  3. Nathaniel FB, Janet G, John SL. Use of the Fricke dosimeter to measure photoelectric absorption. Radiat. Res. 1961;14(3):291-295. https://doi.org/10.2307/3570923
  4. Flick MB, Warters RL, Yasui LS, Krisch RE. Measurement of radiation-induced DNA damage using gel electrophoresis or neutral filter elution shows an increased frequency of DNA strand breaks after exposure to pH 9.6. Radiat. Res. 1989;119(3):452-465. https://doi.org/10.2307/3577517
  5. Calini V, Urani C, Camatini M. Comet assay evaluation of DNA single-and double-strand breaks induction and repair in C3H 10T1/2 cells. Cell Biol. Toxicol. 2002;18(6):369-379. https://doi.org/10.1023/A:1020811522100
  6. Bustos ME, Romero ME, Gutierrez A, Azorin J. Identification of irradiated mangoes by means of ESR spectroscopy. Appl. Radiat. Isot. 1996 Nov;47(11-12):1655-1656. https://doi.org/10.1016/S0969-8043(96)00239-4
  7. Ikeya M, Miyajima J, Okajima S. ESR dosimetry for atomic bomb survivors, using shell buttons and tooth enamel. J. Appl. Phys. 1984;23:L699-710. https://doi.org/10.1143/JJAP.23.699
  8. Ikeya M, Ishii H. Atomic bomb and accident dosimetry with ESR: Natural rocks and human tooth in-vivo spectrometer. Appl. Radiat. Isot. 1989;40:1021-1027. https://doi.org/10.1016/0883-2889(89)90035-X
  9. Rabek JF. Progress in photochemistry & photophysics. 6th Ed. Boca Raton, FL. CRC Press. 1992;43.
  10. Izumi Y, Hirayama M, Matuo Y, Sunagawa T. Microwave dielectric absorption spectroscopy aiming at novel dosimetry using DNAs. J. Radiat. Prot. Res. In press.

Cited by

  1. Microwave Dielectric Absorption Spectroscopy Aiming at Novel Dosimetry Using DNAs vol.42, pp.1, 2017, https://doi.org/10.14407/jrpr.2017.42.1.21