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Tazarotene-Induced Gene 1 Enhanced Cervical Cell Autophagy through Transmembrane Protein 192

  • Shyu, Rong-Yaun (Department of Internal Medicine, Taipei Tzuchi Hospital, The Buddhist Tzuchi Medical Foundation) ;
  • Wang, Chun-Hua (Department of Dermatology, Taipei Tzuchi Hospital, The Buddhist Tzuchi Medical Foundation) ;
  • Wu, Chang-Chieh (Department of Surgery, Tri-Service General Hospital, National Defense Medical Center) ;
  • Chen, Mao-Liang (Department of Research, Taipei Tzuchi Hospital, The Buddhist Tzuchi Medical Foundation) ;
  • Lee, Ming-Cheng (Department of Research, Taipei Tzuchi Hospital, The Buddhist Tzuchi Medical Foundation) ;
  • Wang, Lu-Kai (Radiation Biology Core Laboratory, Institute for Radiological Research, Chang Gung University/Chang Gung Memorial Hospital) ;
  • Jiang, Shun-Yuan (Department of Research, Taipei Tzuchi Hospital, The Buddhist Tzuchi Medical Foundation) ;
  • Tsai, Fu-Ming (Department of Research, Taipei Tzuchi Hospital, The Buddhist Tzuchi Medical Foundation)
  • Received : 2016.06.28
  • Accepted : 2016.11.18
  • Published : 2016.12.31

Abstract

Tazarotene-induced gene 1 (TIG1) is a retinoic acid-inducible protein that is considered a putative tumor suppressor. The expression of TIG1 is decreased in malignant prostate carcinoma or poorly differentiated colorectal adenocarcinoma, but TIG1 is present in benign or well-differentiated tumors. Ectopic TIG1 expression led to suppression of growth in cancer cells. However, the function of TIG1 in cell differentiation is still unknown. Using a yeast two-hybrid system, we found that transmembrane protein 192 (TMEM192) interacted with TIG1. We also found that both TIG1A and TIG1B isoforms interacted and co-localized with TMEM192 in HtTA cervical cancer cells. The expression of TIG1 induced the expression of autophagy-related proteins, including Beclin-1 and LC-3B. The silencing of TMEM192 reduced the TIG1-mediated upregulation of autophagic activity. Furthermore, silencing of either TIG1 or TMEM192 led to alleviation of the upregulation of autophagy induced by all-trans retinoic acid. Our results demonstrate that the expression of TIG1 leads to cell autophagy through TMEM192. Our study also suggests that TIG1 and TMEM192 play an important role in the all-trans retinoic acid-mediated upregulation of autophagic activity.

Keywords

all-trans retinoic acid;autophagy;Beclin-1;LC3B;tazarotene-induced gene 1;transmembrane protein 192

Acknowledgement

Supported by : Buddhist Tzuchi Medical Foundation

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