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Ginsenoside Re Inhibits Osteoclast Differentiation in Mouse Bone Marrow-Derived Macrophages and Zebrafish Scale Model

  • Park, Chan-Mi (World Class Institute (WCI), and Chemical biology Research Center, Korean Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Kim, Hye-Min (World Class Institute (WCI), and Chemical biology Research Center, Korean Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Kim, Dong Hyun (World Class Institute (WCI), and Chemical biology Research Center, Korean Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Han, Ho-Jin (World Class Institute (WCI), and Chemical biology Research Center, Korean Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Noh, Haneul (World Class Institute (WCI), and Chemical biology Research Center, Korean Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Jang, Jae-Hyuk (World Class Institute (WCI), and Chemical biology Research Center, Korean Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Park, Soo-Hyun (Clinical Trial Center for Functional Foods(CTCF2), Chonbuk National University Hospital) ;
  • Chae, Han-Jung (Clinical Trial Center for Functional Foods(CTCF2), Chonbuk National University Hospital) ;
  • Chae, Soo-Wan (Clinical Trial Center for Functional Foods(CTCF2), Chonbuk National University Hospital) ;
  • Ryu, Eun Kyoung (Center of Magnetic Resonance Research, Korea Basic Science Institute) ;
  • Lee, Sangku (World Class Institute (WCI), and Chemical biology Research Center, Korean Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Liu, Kangdong (World Class Institute (WCI), and Chemical biology Research Center, Korean Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Liu, Haidan (World Class Institute (WCI), and Chemical biology Research Center, Korean Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Ahn, Jong-Seog (World Class Institute (WCI), and Chemical biology Research Center, Korean Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Kim, Young Ock (Department of Medicinal Crop Research Institute, National Institute of Horticultural & Herbal Science, Rural Development Administration) ;
  • Kim, Bo-Yeon (World Class Institute (WCI), and Chemical biology Research Center, Korean Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Soung, Nak-Kyun (World Class Institute (WCI), and Chemical biology Research Center, Korean Research Institute of Bioscience and Biotechnology (KRIBB))
  • Received : 2016.04.25
  • Accepted : 2016.11.07
  • Published : 2016.12.31

Abstract

Ginsenosides, which are the active materials of ginseng, have biological functions that include anti-osteoporotic effects. Aqueous ginseng extract inhibits osteoclast differentiation induced by receptor activator of NF-${\kappa}B$ ligand (RANKL). Aqueous ginseng extract produces chromatography peaks characteristic of ginsenosides. Among these peaks, ginsenoside Re is a major component. However, the preventive effects of ginsenoside Re against osteoclast differentiation are not known. We studied the effect of ginsenoside Re on osteoclast differentiation, RANKL-induced tartrate-resistant acid phosphatase (TRAP) activity, and formation of multinucleated osteoclasts in vitro. Ginsenoside Re hampered osteoclast differentiation in a dose-dependent manner. In an in vivo zebrafish model, aqueous ginseng extract and ginsenoside Re had anti-osteoclastogenesis effects. These findings suggest that both aqueous ginseng extract and ginsenoside Re prevent bone resorption by inhibiting osteoclast differentiation. Ginsenoside Re could be important for promoting bone health.

Keywords

ginsenoside Re;osteoclasts;RANKL;zebrafish

Acknowledgement

Supported by : Rural Development Administration

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  1. Ginsenoside Re Promotes Osteoblast Differentiation in Mouse Osteoblast Precursor MC3T3-E1 Cells and a Zebrafish Model vol.22, pp.1, 2016, https://doi.org/10.3390/molecules22010042