- Volume 16 Issue 18
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Cell Cycle Modulation of MCF-7 and MDA-MB-231 by a Sub-Fraction of Strobilanthes crispus and its Combination with Tamoxifen
- Yaacob, Nik Soriani (Department of Chemical Pathology, Universiti Sains Malaysia Health Campus) ;
- Kamal, Nik Nursyazni Nik Mohamed (Department of Chemical Pathology, Universiti Sains Malaysia Health Campus) ;
- Wong, Kah Keng (Department of Immunology, School of Medical Sciences, Universiti Sains Malaysia Health Campus) ;
- Norazmi, Mohd Nor (School of Health Sciences, Universiti Sains Malaysia Health Campus)
- Published : 2016.01.11
Background: Cell cycle regulatory proteins are suitable targets for cancer therapeutic development since genetic alterations in many cancers also affect the functions of these molecules. Strobilanthes crispus (S. crispus) is traditionally known for its potential benefits in treating various ailments. We recently reported that an active sub-fraction of S. crispus leaves (SCS) caused caspase-dependent apoptosis of human breast cancer MCF-7 and MDA-MB-231 cells. Materials and Methods: Considering the ability of SCS to also promote the activity of the antiestrogen, tamoxifen, we further examined the effect of SCS in modulating cell cycle progression and related proteins in MCF-7 and MDA-MB-231 cells alone and in combination with tamoxifen. Expression of cell cycle-related transcripts was analysed based on a previous microarray dataset. Results: SCS significantly caused G1 arrest of both types of cells, similar to tamoxifen and this was associated with modulation of cyclin D1, p21 and p53. In combination with tamoxifen, the anticancer effects involved downregulation of
Strobilanthes crispus;tamoxifen;cell cycle;estrogen receptor;MCF-7;MDA-MB-231
Supported by : USM, Malaysian Ministry of Science, Technology and Innovation
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- Towards the mode of action of Strobilanthes crispus through integrated computational and experimental analyses vol.26, pp.4, 2017, https://doi.org/10.1007/s13562-017-0407-9