Effect of a Mixed Extract of Fenugreek Seeds and Lespedeza cuneata on Testosterone Deficiency Syndrome

호로파와 야관문 복합추출물의 남성갱년기 개선에 관한 연구

  • Received : 2015.01.29
  • Accepted : 2015.06.06
  • Published : 2015.08.31


Testosterone deficiency syndrome (TDS) is normally observed in elderly men and is a clinical and biochemical syndrome, characterized by a decline in plasmic testosterone levels resulting in a significant decrease in quality of life. The aim of this study was to evaluate the effect of a mixed extract of fenugreek seeds and Lespedeza cuneata (YHM) on TDS. Rats were divided into three groups: the negative control, YHM-40 (40 mg/kg), and YHM-80 (80 mg/kg) groups. After 4 weeks of YMH administration, an increase was observed in the plasmic testosterone levels, vastus lateralis muscular strength, forced swimming time, total sperm counts, and motile sperm counts in YHM-40 and YHM-80 groups compared to the negative control group. Moreover, sex hormone binding globulin, the epididymal fat pad, total plasmic cholesterol, and triglyceride levels were significantly decreased in the YHM-fed groups. However, prostate specific antigen, aspartate aminotransferase, and alanine aminotransferase levels did not differ among the groups. These results suggest that YHM may enhance testosterone levels in elderly men and alleviate TDS without common side effects.


  1. Becker HWG. Testicular dysfunction in systemic diseases. pp. 1150-1156. In: Principles and practice of endocrinology and metabolism, Backer KL (ed). Lippincott Co., Philadelphia, PA, USA (2000)
  2. Araujo AB, Esche GR, Kupelian V, O'Donnell AB, Travison TG, Williams RE, Clark RV, McKinlay JB. Prevalence of symptomatic androgen deficiency in men. J. Clin. Endocr. Metab. 92: 4241-4247 (2007)
  3. Buvat J, Maggi M, Gooren L, Guay AT, Kaufman J, Morgentaler A, Schulman C, Tan HM, Torres LO, Yassin A, Zitzmann M. Endocrine aspects of male sexual dysfunctions. J. Sex. Med. 7: 1627-1656 (2010)
  4. Bhasin S, Cunningham GR, Hayes FJ, Matsumoto AM, Snyder PJ, Swerdloff RS, Montori VM. Testosterone therapy in adult men with androgen deficiency syndromes: An endocrine society clinical practice guideline. J. Clin. Endocr. Metab. 91:1995-2010 (2006)
  5. Nieschlag E, Swerdloff R, Behre HM, Gooren LJ, Kaufman JM, Legros JJ, Lunenfeld B, Morley JE, Schulman C, Wang C, Weidner W, Wu FCW. Investigation, treatment, and monitoring of late-onset hypogonadism in males: ISA, ISSAM, and EAU recommendations. J. Androl. 27: 135-137 (2006)
  6. Wang C, Nieschlag E, Swerdloff R, Behre HM, Hellstrom WJ, Gooren LJ, Kaufman JM, Legros JJ, Lunenfeld B, Morales A, Morley JE, Schulman C, Thompson IM, Weidner W, Wu FCW. ISA, ISSAM, EAU, EAA and ASA recommendations: Investigation, treatment and monitoring of late-onset hypogonadism in males. Aging Male 12: 5-12 (2009)
  7. Singh P. Andropause: Current concepts. Indian J. Endocrinol. Metab. 17 (Suppl 3): S621-629 (2013)
  8. Bhasin S, Cunningham GR, Hayes FJ, Matsumoto AM, Snyder PJ, Swerdloff RS, Montori VM. Testosterone therapy in men with androgen deficiency syndromes: An Endocrine Society clinical practice guideline. J. Clin. Endocr. Metab. 95: 2536-2559 (2010)
  9. Werner AA. The male climacteric: Report of two hundred and seventy-three casas. J. Amer. Med. Assoc. 132: 188-194 (1946)
  10. Shores MM, Matsumoto AM. Testosterone, aging and survival: biomarker or deficiency. Curr. Opin. Endocrinol. Diabetes Obes. 21: 209-216 (2014)
  11. Gray A, Feldman HA, McKinlay JB, Longcope C. Age, disease, and changing sex hormone levels in middle-aged men: Results of the Massachusetts male aging study. J. Clin. Endocr. Metab. 73: 1016-1025 (1991)
  12. Harman SM, Metter EJ, Tobin JD, Pearson J, Blackman MR. Longitudinal effects of aging on serum total and free testosterone levels in healthy men. Baltimore Longitudinal Study of Aging. J. Clin. Endocr. Metab. 86: 724-731 (2001)
  13. Wu FCW, Tajar A, Pye SR, Silman AJ, Finn JD, O'Neill TW, Bartfai G, Casanueva F, Forti G, Giwercman A, Huhtaniemi IT, Kula K, Punab M, Boonen S, Vanderschueren D. Hypothalamicpituitary-testicular axis disruptions in older men are differentially linked to age and modifiable risk factors: The European male aging study. J. Clin. Endocr. Metab. 93: 2737-2745 (2008)
  14. Gruenewald DA, Matsumoto AM. Testosterone supplementation therapy for older men: Potential benefits and risks. J. Am. Geriatr. Soc. 51: 101-115 (2003)
  15. Rhoden EL, Morgentaler A. Risks of testosterone-replacement therapy and recommendations for monitoring. New Engl. J. Med. 350: 482-1492 (2004)
  16. Muller M, van der Schouw YT, Thijssen JHH, Grobbee DE. Endogenous sex hormones and cardiovascular disease in men. J. Clin. Endocr. Metab. 88: 5076-5086 (2003)
  17. Duru M, Erdogan Z, Duru A, Kucukgul A, Duzguner V, Kaya DA, Sahin A. Effect of seed powder of a herbal legume fenugreek (Trigonella foenum-graceum L.) on growth performance, body components, digestive parts, and blood parameters of broiler chicks. Pakistan J. Zool. 45: 1007-1014 (2013)
  18. Yadav UC, Baquer NZ. Pharmacological effects of Trigonella foenum-graecum L. in health and disease. Pharm. Biol. 52: 243-254 (2014)
  19. Kassem A, Al-Aghbari A, AL-Habori M, Al-Mamary M. Evaluation of the potential antifertility effect of fenugreek seeds in male and female rabbits. Contraception 73: 301-306 (2006)
  20. Aswar U, Bodhankar SL, Mohan V, Thakurdesai PA. Effect of furostanol glycosides from Trigonella foenum-graecum on the reproductive system of male albino rats. Phytother. Res. 24: 1482-1488 (2010)
  21. Lee JK, Kang DG, Lee HS. Vascular relaxation induced by aqueous extract of Lespedeza cuneata via the NO-cGMP pathway. J. Nat. Med. 66: 17-24 (2012)
  22. Park SY, Kim HH. Effect of Lespedezea cuneata on the contraction of rabbit common carotid artery and corpus cavernosum. Korean J. Orient. Physiol. Pathol. 27: 809-817 (2013)
  23. Isidori AM, Giannetta E, Gianfrilli D, Greco EA, Bonifacio V, Aversa A, Isidori A, Fabbri A, Lenzi A. Effects of testosterone on sexual function in men: Results of a meta-analysis. J. Clin. Endocrinol. 63: 381-394 (2005)
  24. Derby CA, Zilber S, Brambilla D, Morales KH, McKinlay JB. Body mass index, waist circumference and waist to hip ratio and change in sex steroid hormones: The Massachusetts male ageing study. J. Clin. Endocrinol. 65 :125-131 (2006)
  25. Morales A, Schulman CC, Tostain J, Wu FC. Testosterone deficiency syndrome (TDS) needs to be named appropriately: The importance of accurate terminology. Eur. Urol. 50: 407-409 (2006)
  26. Taylor WG, Elder JL, Chang PR, Richards KW. Microdetermination of diosgenin from fenugreek (Trigonella foenum-graecum) seeds. J. Agr. Food Chem. 48: 5206-5210 (2000)
  27. Sato K, Fujita S, Iemitsu M. Acute administration of diosgenin or dioscorea improves hyperglycemia with increases muscular steroidogenesis in STZ-induced type 1 diabetic rats. J. Steroid Biochem. 143: 152-159 (2014)
  28. Murakami T, Kishi A, Matsuda H, Yoshikawa M. Medicinal foodstuffs XVII. Fenugreek seed. (3): Structures of new furostanol-type steroid saponins, trigoneosides Xa, Xb, XIb, XIIa, XIIb, and XIIIa, from the seeds of egyptian trigonella foenumgraecum L. Chem. Pharm. Bull. 48: 994-1000 (2000)
  29. Shea JL, Wong PY, Chen Y. Free testosterone: Clinical utility and important analytical aspects of measurement. Adv. Clin. Chem. 63: 59-84 (2014)
  30. Hammond GL. Diverse roles for sex hormone-binding globulin in reproduction. Biol. Reprod. 85: 431-441 (2011)
  31. Duffy MJ. PSA in screening for prostate cancer: More good than harm or more harm than good? Adv. Clin. Chem. 66: 1-23 (2014)
  32. Rick FG, Schally AV. Bench-to-bedside development of agonists and antagonists of luteinizing hormone-releasing hormone for treatment of advanced prostate cancer. Urol. Oncol.-Semin. O. I. 33: 270-274 (2015)
  33. Barqawi A, Crawford ED. Testosterone replacement therapy and the risk of prostate cancer. Is there a link? Int. J. Impot. Res. 18: 323-328 (2006)
  34. Calof OM, Singh AB, Lee ML, Kenny AM, Urban RJ, Tenover JL, Bhasin S. Adverse events associated with testosterone replacement in middle-aged and older men: A meta-analysis of randomized, placebo-controlled trials. J. Gerontol. A-Biol. 60: 1451-1457 (2005)
  35. Schubert M, Jockenhovel F. Late-onset hypogonadism in the aging male (LOH): definition, diagnostic and clinical aspects. J. Endocrinol. Invest. 28: 23-27 (2005)
  36. Seo SM, Jeong YS, Hari DK, Shin DH, Lee IJ, Park ES, Lee JD, Hwang YH. Variation of pinitol content for domestic legume species in korea. Korean J. Crop. Sci. 56: 50-56 (2011)
  37. Kerksick CM, Wilborn CD, Campbell WI, Harvey TM, Marcello BM, Roberts MD, Parker AG, Byars AG, Greenwood LD, Almada AL, Kreider RB, Greenwood M. The effects of creatine monohydrate supplementation with and without D-pinitol on resistance training adaptations. J. Strength Cond. Res. 23: 2673-2682 (2009)
  38. Schroeder, ET, Terk M, Sattler FR. Androgen therapy improves muscle mass and strength but not muscle quality: Results from two studies. Am. J. Physiol.-Endoc. M. 285: E16-E24 (2003)
  39. Schroeder ET, Vallejo AF, Zheng L, Stewart Y, Flores C, Nakao S, Martinez C, Sattler FR. Six-week improvements in muscle mass and strength during androgen therapy in older men. J. Gerontol. A-Biol. 60: 1586-1592 (2005).
  40. Moss JL, Crosnoe LE, Kim ED. Effect of rejuvenation hormones on spermatogenesis. Fertil. Steril. 99: 1814-1820 (2013)
  41. Kovac J, Pastuszak AW, Lamb DJ, Lipshultz LI. Testosterone supplementation therapy in the treatment of patients with metabolic syndrome. Postgrad. Med. 126: 149-156 (2014)
  42. Grober ED. Testosterone deficiency and replacement: Myths and realities. Cuaj-Can. Urol. Assoc. 8: S145-S147 (2014)
  43. Kelly DM, Jones TH. Testosterone and cardiovascular risk in men. Front. Horm. Res. 43: 1-20 (2014)
  44. Volpato S, Vigna GB, Fellin R. The benefit and risk of testosterone replacement therapy in older men: Effects on lipid metabolism. Acta Biomed. 81: 95-99 (2010)
  45. Singh AB, Tamarkar AK, Narender T, Srivastava AK. Antihyperglycaemic effect of an unusual amino acid (4-hydroxyisoleucine) in C57BL/KsJ-db/db mice. Nat. Prod. Res. 24: 258-265 (2010)
  46. Haeri MR, Limaki HK, Branford White CJ, White KN. Noninsulin dependent anti-diabetic activity of (2S, 3R, 4S) 4-hydroxyisoleucine of fenugreek (Trigonella foenum graecum) in streptozotocin-induced type I diabetic rats. Phytomedicine 19: 571-574 (2012)
  47. Vinken M. The adverse outcome pathway concept: A pragmatic tool in toxicology. Toxicology 4: 158-165 (2013)

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