Single Nucleotide Polymorphisms in miR-149 (rs2292832) and miR-101-1 (rs7536540) Are Not Associated with Hepatocellular Carcinoma in Thai Patients with Hepatitis B Virus Infection

  • Pratedrat, Pornpitra (Department of Biochemistry, Faculty of Medicine, Chulalongkorn University) ;
  • Sopipong, Watanyoo (Department of Biochemistry, Faculty of Medicine, Chulalongkorn University) ;
  • Makkoch, Jarika (Department of Biochemistry, Faculty of Medicine, Chulalongkorn University) ;
  • Praianantathavorn, Kesmanee (Department of Biochemistry, Faculty of Medicine, Chulalongkorn University) ;
  • Chuaypen, Natthaya (Department of Biochemistry, Faculty of Medicine, Chulalongkorn University) ;
  • Tangkijvanich, Pisit (Department of Biochemistry, Faculty of Medicine, Chulalongkorn University) ;
  • Payungporn, Sunchai (Department of Biochemistry, Faculty of Medicine, Chulalongkorn University)
  • Published : 2015.10.06


MicroRNAs directly and indirectly influence many biological processes such as apoptosis, cell maintenance, and immune responses, impacting on tumor genesis and metastasis. They modulate gene expression at the posttranscriptional level and are associated with progression of liver disease. Hepatocellular carcinoma (HCC) is a cancer which mostly occurs in males. There are many factors affect HCC development, for example, hepatitis B virus (HBV), hepatitis C virus (HCV) and human immunodeficiency virus (HIV), co-infection, environmental factors including alcohol, aflatoxin consumption and host-related factors such as age, gender immune response, microRNA and single nucleotide polymorphisms (SNPs). Chronic infection with the hepatitis B virus is the major factor leading to HCC progression since it causes the liver injury. At present, there are many reports regarding the association of SNPs on miRNAs and the HCC progression. In this research, we investigated the role of miR-149 (rs2292832) and miR-101-1 (rs7536540) with HCC progression in Thai population. The study included 289 Thai subjects including 104 HCC patients, 90 patients with chronic hepatitis B virus infection (CHB) and 95 healthy control subjects. The allele and genotype of rs2292832 and rs7536540 polymorphisms were determined by TaqMan real-time PCR assay. Our results revealed no significant association between miR-149 (rs2292832) and miR-101-1 (rs7536540) and the risk of HCC in our Thai population. However, this research is the first study of miR-149 (rs2292832) and miR-101-1 (rs7536540) in HCC in Thai populations and the results need to be confirmed with a larger population.


Supported by : Chulalongkorn University


  1. Akkiz H, Bayram S, Bekar A, et al (2011). Genetic variation in the microRNA-499 gene and hepatocellular carcinoma risk in a Turkish population: lack of any association in a case-control study. Asian Pac J Cancer Prev, 12, 3107-12.
  2. Bae JS, Kim JH, Pasaje CF, et al (2012). Association study of genetic variations in microRNAs with the risk of hepatitis B-related liver diseases. Dig Liver Dis, 44, 849-54.
  3. Chen CZ (2005). MicroRNAs as oncogenes and tumor suppressors. N Engl J Med, 353, 1768-71.
  4. El-Serag HB, Rudolph KL (2007). Hepatocellular carcinoma: epidemiology and molecular carcinogenesis. Gastroenterology, 132, 2557-76.
  5. Jemal A, Bray F, Center MM, et al (2011). Global cancer statistics. CA Cancer J Clin, 61, 69-90.
  6. Kadam P, Bhalerao S (2010). Sample size calculation. Int J Ayurveda Res, 1, 55-7.
  7. Kim WH, Min KT, Jeon YJ, et al (2012). Association study of microRNA polymorphisms with hepatocellular carcinoma in Korean population. Gene, 504, 92-7.
  8. Kwak MS, Lee DH, Cho Y, et al (2012). Association of polymorphism in pri-microRNAs-371-372-373 with the occurrence of hepatocellular carcinoma in hepatitis B virus infected patients. PloS one, 7, 41983.
  9. Li S, Fu H, Wang Y, et al (2009). MicroRNA-101 regulates expression of the v-fos FBJ murine osteosarcoma viral oncogene homolog (FOS) oncogene in human hepatocellular carcinoma. Hepatology, 49, 1194-202.
  10. Li XD, Li ZG, Song XX, et al (2010). A variant in microRNA-196a2 is associated with susceptibility to hepatocellular carcinoma in Chinese patients with cirrhosis. Pathology, 42, 669-73.
  11. Liu Y, Zhang Y, Wen J, et al (2012). A genetic variant in the promoter region of miR-106b-25 cluster and risk of HBV infection and hepatocellular carcinoma. PloS one, 7, 32230.
  12. Pan SJ, Zhan SK, Pei BG et al (2012). MicroRNA-149 inhibits proliferation and invasion of glioma cells via blockade of AKT1 signaling. Int J Immunopathol Pharmacol, 25, 871-81.
  13. Parkin DM, Whelan SL, Ferlay J, et al (2003). Cancer Incidence In five continents. Vol. VIII. No.155. Lyon: IARCPress.
  14. Perz JF, Armstrong GL, Farrington LA, et al (2006). The contributions of hepatitis B virus and hepatitis C virus infections to cirrhosis and primary liver cancer worldwide. J Hepatol, 45, 529-38.
  15. Ramirez-Bello J, Vargas-Alarcon G, Tovilla-Zarate C, et al (2013). Single nucleotide polymorphisms (SNPs): functional implications of regulatory-SNP (rSNP) and structural RNA (srSNPs) in complex diseases. Gac Med Mex, 149, 220-8.
  16. Sopipong W, Tangkijvanich P, Payungporn S, et al (2013). The KIF1B (rs17401966) single nucleotide polymorphism is not associated with the development of HBV-related hepatocellular carcinoma in Thai patients. Asian Pac J Cancer Prev, 14, 2865-9.
  17. Tangkijvanich P, Hirsch P, Theamboonlers A, et al (1999). Association of hepatitis viruses with hepatocellular carcinoma in Thailand. J Gastroenterol, 34, 227-33.
  18. Thomas LF, Saetrom P (2012). Single nucleotide polymorphisms can create alternative polyadenylation signals and affect gene expression through loss of microRNA-regulation. PLoS Comput Biol, 8, 1002621.
  19. Wang R, Zhang J, Ma Y, et al (2014). Association study of miR-149 rs2292832 and miR-608 rs4919510 and the risk of hepatocellular carcinoma in a large-scale population. Mol Med Rep, 10, 2736-44.
  20. Wang Y, Zheng X, Zhang Z, et al (2012). MicroRNA-149 inhibits proliferation and cell cycle progression through the targeting of ZBTB2 in human gastric cancer. PloS one, 7, 41693.
  21. Xiang Y, Fan S, Cao J, et al (2012). Association of the microRNA-499 variants with susceptibility to hepatocellular carcinoma in a Chinese population. Mol Biol Rep, 39, 7019-23.

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