- Volume 16 Issue 11
DOI QR Code
Secondary Circulating Tumor Cells (CTCs) but not Primary CTCs are Associated with the Clinico-Pathological Parameters in Chilean Patients With Colo-Rectal Cancer
- Murray, Nigel P (Hematology, Hospital de Carabineros de Chile, Faculty of Medicine, University Finis Terrae) ;
- Albarran, Vidal (Coloproctology, Hospital de Carabineros de Chile) ;
- Perez, Guillermo (Coloproctology, Hospital de Carabineros de Chile) ;
- Villalon, Ricardo (Pathology, Hospital de Carabineros de Chile) ;
- Ruiz, Amparo (Pathology, Hospital de Carabineros de Chile)
- Published : 2015.06.26
Background: The aim of this study was to assess detection of circulating tumor cells (CTC) using anti-CEA pre and post surgery in Chilean patients with colo-rectal cancer. Materials and Methods: The presence of CTCs was evaluated in 80 colorectal cancer patients pre and post surgery using standard immunocytochemistry and the results were compared with findings for standard clinico-pathological parameters. Results: In patients presurgery CEA (+) CTCs were frequently found, with no relation to tumor size or nodal status. After surgery, the presence of CTCs was associated with such clinico-pathological parameters. The frequency of CTC detection in node positive patients did not change after surgery. In patients with metastasis there was also no change in the frequency of CTC detection, and clusters of 3 or more CTCs were evident. Conclusions: Secondary CTCs are associated with clinico-pathological parameters only after surgical removal of the primary tumor, and might be important in identifying patients at high risk of relapse. Primary CTCs detected before surgical removal are frequently found, are not associated with the clinico-pathological parameters and might have a role in cancer screening. These findings suggest the need for studies with a larger population of patients.
Colorectal cancer;circulating tumor cells;CEA
- Albuquerque A, Kubisch I, Stolzel U, et al (2012). Prognostic and predictive value of CTC analysis in colorectal cancer patients. J Translat Med, 10, 222-8. https://doi.org/10.1186/1479-5876-10-222
- Abdifard E, Ghaderi S, Hosseini S et al (2013). Incidence trends of colorectal cancer in the west of Iran during 2000-2005. Asian Pac J Cancer Prev, 14, 1807-11. https://doi.org/10.7314/APJCP.2013.14.3.1807
- Albuquerque A, Kubisch I, Ernest D et al (2012a). Development of a molecular multimarker assay for the analysis of CTCs in adenocarcinoma patients. Clin Lab, 58, 373-84.
- Albuquerque, Kubisch I, Breier G et al (2012b). Multimarker gene analysis of CTCs in pancreatic cancer patients, a feasibility study. Oncol, 82, 3-10. https://doi.org/10.1159/000335479
- Allard J, Matera J, Miller MC et al (2004). Tumor cells circulate in the peripheral blood of all major carcinomas but not in healthy subjects or patients with non-malignant disease. Clin Cancer Res, 10, 6897-904. https://doi.org/10.1158/1078-0432.CCR-04-0378
- Ashworth TR (1869). A case of cancer in which cells similar to those in the tumours were seen in the blood after death. Med J Aust, 14, 146-9.
- Borgen E, Naume B, Nesland JM, et al (1999). Standardization of the immunocytochemical detection of cancer cells in bone marrow and blood, Establishment of objective criteria for the evaluation of immunostained cells. Cytotherapy, 5, 377-88.
- Edge SB, Byrd DR, Compton CC, et al (2010). AJCC Cancer Staging Manual 7th Ed. Springer Verlag, New York NY, USA.
- Fidler IJ (1970). Metastasis, Quantitative analysis of didsribution and fate of tumor emboli labelled with 125 I-5-yodo-2'-deoxyuridine. J Natl Cancer Inst, 45, 773-82.
- Fidler IJ (1973). The relationship of embolic homogenicity, number ,size and viability to the incidence of experimental metastasis. Eur J Cancer, 9, 223-227. https://doi.org/10.1016/S0014-2964(73)80022-2
- Galizia G, Gemei M, Orditura M, et al (2013). Postoperative detection of CTCs predicts tumor recurrence in colorectal cancer patients. J Gastrointest Surg, 17, 1809-18. https://doi.org/10.1007/s11605-013-2258-6
- Gazzaniga P, Gianni W, Raimondi C, et al (2013). CTCs in risk risk nonmetastatic colorectal cancer. Tumour Biol, 34, 2507-9. https://doi.org/10.1007/s13277-013-0752-9
- Glaves D (1983). Correlation between circulating cancer cells and incidence of metastasis. Br J Cancer, 48, 665-673. https://doi.org/10.1038/bjc.1983.248
- Hayes DF, Smerage J (2008). Is there a role for CTCs in the management of breast cancer? Clin Cancer Res, 14, 3646-3650 https://doi.org/10.1158/1078-0432.CCR-07-4481
- Huang X, Gao P, Song Y, et al (2014). Relationship between circulating tumorcells and tumor response in colorectal cancer patients treated with chemotherapy, a meta-analysis. BMC Cancer, 14, 976-91. https://doi.org/10.1186/1471-2407-14-976
- Huang X, Gao P, Song Y, et al (2015). Meta-analysis of the prognostic value of circulatingtumor cells detected with the CellSearch System in colorectal cancer. BMC Cancer, 15, 202. https://doi.org/10.1186/s12885-015-1218-9
- Kojima T, Yoshikawa K, Maisui T, et al (2009). Titration of serum CEA, p53 antibodies and CEAIgM complexes in patients with colorectal cancer. Mol Med Rep, 2, 477-80.
- Lim SH, Becker TM, Chua W, et al (2014). CTCs andcircualting free nucleaic acid as prognostic and predictive biomarkers in colorectal cancer. Cancer Lett, 346, 24-33. https://doi.org/10.1016/j.canlet.2013.12.019
- Mikolajczyk SD, Miller LS, Tsinberg P, et al (2011). Detection of EpCAM negative and cytokeratin negative circulating tumor cells in peripheral blood. J Oncol, [Epub ahead of print].
- Molner B, Sipos F, Galamb O, et al (2003). Molecular detection of circulating cancer cells, Role in diagnosis, prognosis and follow up of colon cancer patients. Dig Dis, 21, 320-325 https://doi.org/10.1159/000075355
- Murray NP, Reyes E, Badinez L, et al (2013). Circulating prostate cells found in men with benign prostate disease are P504S negative, clinical implications. J Oncol, [Epub ahead of print].
- Musella V, Pietrantonio E, DinBuduo E, et al (2015). CTCs as a longitudinal biomarker in patoients wit hadvanced chemorefractory, RAS-BRAF wild type colorectal cancer receiving cetuximab or panitumumab. Int J Cancer, [Epub ahead of print]
- Pantel K, Deneve E, D'Nocca D, et al (2012). Circulating epitelial cells in patients with benign colon disease. Clin Chem, 58, 936-40. https://doi.org/10.1373/clinchem.2011.175570
- Patel H, Le Marr N, Wharton RQ, et al (2002). Clearance of circulating tumor cells after excision of primary colorectal cancer. Ann Surg, 2, 226-231.
- Raimondi C, Gradilone A, Naso G, et al (2011). Epithelial-mesenchyme transition and stemness features in circulating tumor cells from breast cancer patients. Breast Cancer Res Treat, 130, 449-55. https://doi.org/10.1007/s10549-011-1373-x
- Shively JE, Beatty JD (1985). CEA related antigens, molecular biology and clinical significance. Crit Rev Oncol Hematol, 2, 89-139.
- Suhaimia NA, Foong YM, Lee DY, et al (2015). Non-invasive sensitive detection of KRAS and BRAF mutation in circulating tumor cells of colorectal cancer patients. Mol Oncol, 9, 850-60. https://doi.org/10.1016/j.molonc.2014.12.011
- Van Dalum G, Stam GJ, Scholten LF, et al (2015). Importance of circulating tumor cells in newly diagnosed colorectal cancer. Int J Oncol, 46, 1361-8. https://doi.org/10.3892/ijo.2015.2824
- Weitz J, Kienle P, Lacroix J, et al (1998). Dissemination of tumor cells in patients undergoing surgery for colorectal cancer. Clin Cancer Res, 4, 343-8.
- Wharton RQ, Jonas SK, Glover C, et al (1999). Increased detection of CTCs in blood of CRC patients using two rT-PCR assays and multiple blood sampling. Clin Cancer Res, 5, 4158-63.
- Yang XQ, Chen C, Wang FB, et al (2011). Preoperative serum carinoembryonic antigen, carbohydrate antigen 19-9 and carbohydrate antigen 125 as prognostic factors for recurrence free survival in colorectal cancer. Asian Pac J Cancer Prev, 12, 1251-6.
- Zheng ZX, Zheng RS, Zhang SW, et al (2014). Colorectal cancer incidence and mortality in China, 2010. Asian Pac J Cancer Prev, 15, 8455-60. https://doi.org/10.7314/APJCP.2014.15.19.8455
- Engell H (1955). Cancer cells circulating in blood. Acta Chir Scand, 1, 201.
- Phenotype of circulating tumor cell: face-off between epithelial and mesenchymal masks vol.37, pp.5, 2016, https://doi.org/10.1007/s13277-016-4796-5