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In vitro Evaluation of Cytotoxic Activities of Essential Oil from Moringa oleifera Seeds on HeLa, HepG2, MCF-7, CACO-2 and L929 Cell Lines

  • Elsayed, Elsayed Ahmed (Department of Zoology, Bioproducts Research Chair, Faculty of Science, King Saud University) ;
  • Sharaf-Eldin, Mahmoud A. (Sara bint Rached bin Ghonaim Research Chair for Cultivating Non-traditional Medicinal and Aromatic Plants, Biology Department, College of Science and Humanities, Prince Sattam bin Abdulaziz University) ;
  • Wadaan, Mohammad (Department of Zoology, Bioproducts Research Chair, Faculty of Science, King Saud University)
  • Published : 2015.06.26

Abstract

Moringa oleifera Lam. (Moringaceae) is widely consumed in tropical and subtropical regions for their valuable nutritional and medicinal characteristics. Recently, extensive research has been conducted on leaf extracts of M. oleifera to evaluate their potential cytotoxic effects. However, with the exception of antimicrobial and antioxidant activities, little information is present on the cytotoxic activity of the essential oil obtained from M. oleifera seeds. Therefore, the present investigation was designed to investigate the potential cytotoxic activity of seed essential oil obtained from M. oleifera on HeLa, HepG2, MCF-7, CACO-2 and L929 cell lines. The different cell lines were subjected to increasing oil concentrations ranging from 0.15 to 1 mg/mL for 24h, and the cytotoxicity was assessed using MTT assay. All treated cell lines showed a significant reduction in cell viability in response to the increasing oil concentration. Moreover, the reduction depended on the cell line as well as the oil concentration applied. Additionally, HeLa cells were the most affected cells followed by HepG2, MCF-7, L929 and CACO-2, where the percentages of cell toxicity recorded were 76.1, 65.1, 59.5, 57.0 and 49.7%, respectively. Furthermore, the $IC_{50}$ values obtained for MCF-7, HeLa and HepG2 cells were 226.1, 422.8 and $751.9{\mu}g/mL$, respectively. Conclusively, the present investigation provides preliminary results which suggest that seed essential oil from M. oleifera has potent cytotoxic activities against cancer cell lines.

Keywords

Anticancer agents;cytotoxicity;medicinal plants;Moringa;seed oil

Acknowledgement

Supported by : Prince Sattam bin Abdulaziz University

References

  1. Abdulkarim SM, Lai OM, Muhammad SKS, Long K, Ghazali HM (2005). Some physic-chemical properties of Moringa oleifera seed oil extracted using solvent and aqueous enzymatic methods. Food Chem, 93, 253-63. https://doi.org/10.1016/j.foodchem.2004.09.023
  2. Al-Ahmadi K, Al-Zahrani A (2013). Spatial autocorrelation of cancer incidence in Saudi Arabia. Int J Environ Res Public Health, 10, 7207-28. https://doi.org/10.3390/ijerph10127207
  3. Alghamdi IG, Hussain II, Alghamdi MS, El-Sheemy MA (2013). The incidence rate of female breast cancer in Saudi Arabia: an observational descriptive epidemiological analysis of data from Saudi cancer registry 2001-2008. Breast Cancer: Targets Therapy, 5, 103-9.
  4. Al-Kuraya KS, Bavi PP, Ezzat AA, et al (2006). Colorectal carcinoma from Saudi Arabia: Analysis of MLH-1, MSH-2 and p53 genes by immunohistochemistry and tissue microarray analysis. Saudi Med J, 27, 323-8.
  5. Al-Oqail MM, Farshori NN, Al-Sheddi ES, et al (2013). In vitro cytotoxic activity of seed oil of fenugreek against various cancer cell cines. Asian Pac J Cancer Prev, 14, 1829-32. https://doi.org/10.7314/APJCP.2013.14.3.1829
  6. Alsbeih G, Al-Harbi N, El-Sebaie M, Al-Badawi I (2013). HPV prevalence and genetic predisposition to cervical cancer in Saudi Arabia. Infect Agents Cancer, 8, 15. https://doi.org/10.1186/1750-9378-8-15
  7. Al-Sheddi ES, Farshori NN, Al-Oqail MM, et al (2014). Cytotoxicity of Nigella Sative seed oil and extract against human lung cancer cell line. Asian Pac J Cancer Prev, 15, 983-7. https://doi.org/10.7314/APJCP.2014.15.2.983
  8. Anwar F, Latif S, Ashraf M, Gilani AH (2007). Moringa oleifera: a food plant with multiple-medicinal uses. Phytother Res, 21, 17-25. https://doi.org/10.1002/ptr.2023
  9. Awodele O, Oreagbaa IA, Odomaa S, Teixeira da Silva JA, Osunkaluc VO (2012). Toxicological evaluation of the aqueous leaf extract of Moringa oleifera Lam. (Moringaceae). J Ethnopharmacol, 139, 330-6. https://doi.org/10.1016/j.jep.2011.10.008
  10. Bakar MFA, Mohamad M, Rahmat A, Burr SA, Fry JR (2010). Cytotoxicity, cell cycle arrest, and apoptosis in breast cancer cell lines exposed to an extract of the seed kernel of Mangifera pajang (bambangan). Food Chem Toxicol, 48, 1688-97. https://doi.org/10.1016/j.fct.2010.03.046
  11. Chuang P-H, Lee C-W, Chou J-C, et al (2007). Anti-fungal activity of crude extracts and essential oil of Moringa oleifera Lam. Bioresour Technol, 98, 232-6. https://doi.org/10.1016/j.biortech.2005.11.003
  12. Cragg GM, Newman DJ (2005). Plant as source of anticancer agents. J Ethnopharmacol, 100, 72-9. https://doi.org/10.1016/j.jep.2005.05.011
  13. Farooq M, Hozzein WN, Elsayed EA, et al (2013). Identification of histone deacetylase I protein complexes in liver cancer cells. Asian Pac J Cancer Prev, 14, 915-21. https://doi.org/10.7314/APJCP.2013.14.2.915
  14. Farshori NN, Al-Sheddi ES, Al-Oqail MM, et al (2013). Anticancer activity of Petroselinum sativum seed extracts on MCF-7 human breast cancer cells. Asian Pac J Cancer Prev, 14, 5719-23. https://doi.org/10.7314/APJCP.2013.14.10.5719
  15. Ferlay J, Soerjomataram I, Dikshit R, et al (2014). Cancer incidence and mortality worldwide: sources, methods and major patterns in GLOBOCAN 2012. Inter J Cancer, 136, 359-86.
  16. Ferreira RS, Napoleao TH, Santos AF, et al (2011). Coagulant and antibacterial activities of the water-soluble seed lectin from Moringa oleifera. Lett Appl Microbiol, 53, 186-92. https://doi.org/10.1111/j.1472-765X.2011.03089.x
  17. Gasparotto JrA, Gasparotto FM, Lourenco ELB, et al (2011). Anti-hypertensive effects of isoquercitin and extracts from Tropaeolum majus L: evidence for the inhibition of angiotensin converting enzyme. J Ethnopharmcol, 134, 363-72. https://doi.org/10.1016/j.jep.2010.12.026
  18. Ghazali HM, Mohammed AS (2011). Moringa (Moringa oleifera) seed oil: composition, nutritional aspects and health attributes. in 'nuts & seeds in health and disease prevention', Eds. Preedy V, Watson R and Patel V. Elsevier Academic Press, USA pp 787-93.
  19. Hannan Md.A, Kang J-Y, Mohibbullah Md, et al (2014). Moringa oleifera with promising neuronal survival and neurite outgrowth promoting potentials. J Ethnopharmacol, 152, 142-50. https://doi.org/10.1016/j.jep.2013.12.036
  20. Heo B-G, Park Y-J, Park Y-S, et al (2014). Anticancer and antioxidant effects of extracts from different parts of indigo plant. Indust Crops Products, 56, 9-16. https://doi.org/10.1016/j.indcrop.2014.02.023
  21. Huang Y-J, Zhang Y-Y, Liu G, et al (2014). Preliminary evaluation of the in vitro efficacy of 1, 2-di(quinazolin-4-yl) diselane against SiHa cervical cancer cells. Asian Pac J Cancer Prev, 15, 6301-6. https://doi.org/10.7314/APJCP.2014.15.15.6301
  22. Janick J, Paull RE (2008). The encyclopedia of fruit & nuts. CABI, Cambridge, UK pp 509-10.
  23. Kuete V, Krusche B, Youns M, et al (2011). Cytotoxicity of some Cameroonian spices and selected medicinal plant extracts. J Ethnopharmacol, 134, 803-12. https://doi.org/10.1016/j.jep.2011.01.035
  24. Mustafa J, Khan SI, Ma G, Walker LS, Khana IA (2004). Synthesis and anticancer activities of fatty acid analogs of podophyllotoxin. Lipids, 39, 167-72. https://doi.org/10.1007/s11745-004-1215-5
  25. Rashid U, Anwar F, Moser BR, Knothe G (2008). Moringa oleifera: A possible source for biodiesel. Bioresour Technol, 99, 8175-9. https://doi.org/10.1016/j.biortech.2008.03.066
  26. Ravichandran D, Johnson CD (1999). Anticancer effects of essential fatty acids. in 'pancreatic disease', Eds. Johnson CD. Springer-Verlag, London pp 325-37.
  27. Satish A, Kumar RP, Rakshith D, Satish S, Ahmed F (2013). Antimutagenic and antioxidant activity of Ficus benghalensis stem bark and Moringa oleifera root extract. Inter J Chem Anal Sci, 4, 45-8. https://doi.org/10.1016/j.ijcas.2013.03.008
  28. Scheim DE (2009). Cytotoxicity of unsaturated fatty acids in fresh human tumor explants: concentration thresholds and implications for clinical efficacy. Lipids Health Dis, 8, 54. https://doi.org/10.1186/1476-511X-8-54
  29. Shahat AA, Alsaid MS, Alyahya MA, Higgins M, Dinkova-Kostova AT (2013). NAD(P)H: Quinone oxidoreductase 1 inducer activity of some Saudi Arabian medicinal plants. Planta Med, 79, 459-64. https://doi.org/10.1055/s-0032-1328322
  30. Siddhuraju P, Becker K (2003). Antioxidant properties of various solvent extracts of total phenolic constituents from three different agro-climatic origins of drumstick tree (M. oleifera Lam.). J Agric Food Chem, 51, 2144-55. https://doi.org/10.1021/jf020444+
  31. Siddiqui MA, Singh G, Kashyap MP, et al (2008). Influence of cytotoxic doses of 4-ydroxynonenal on selected neurotransmitter receptors in PC-12 cells. Toxicol in vitro, 22, 1681-8. https://doi.org/10.1016/j.tiv.2008.07.001
  32. Sreelatha S, Jeyachitra A, Padma PR (2011). Antiproliferation and induction of apoptosis by Moringa oleifera leaf extract on human cancer cells. Food Chem Toxicol, 49, 1270-5. https://doi.org/10.1016/j.fct.2011.03.006
  33. Sultana S, Asif HM, Nazar HMI, et al (2014). Medicinal plants combating against cancer-a green anticancer approach. Asian Pac J Cancer Prev, 15, 4385-94. https://doi.org/10.7314/APJCP.2014.15.11.4385
  34. Srisuwan S, Hamontri S, Kongsomboon K, et al (2014). See-and-treat approach to cervical intraepithelial lesions in HRH Princess Maha Chakri Sirindhorn Medical Center. Asian Pac J Cancer Prev, 15, 3483-6. https://doi.org/10.7314/APJCP.2014.15.8.3483
  35. Tiloke C, Phulukdaree A, Chuturgoon AA (2013). The antiproliferative effect of Moringa oleifera crude aqueous leaf extract on cancerous human alveolar epithelial cells. BMC Complement Altern Med, 13, 226. https://doi.org/10.1186/1472-6882-13-226
  36. Vijayarathna S, Sreenivasan S (2012). Cytotoxicity of methanol extracts of Elaeis guineensis on MCF-7 and Vero cell lines. Asian Pac J Trop Biomed, 2, 826-9. https://doi.org/10.1016/S2221-1691(12)60237-8
  37. Vongsak B, Sithisarn P, Mangmool S, et al (2013a). Maximizing total phenolics, total flavonoids contents and antioxidant activity of Moringa oleifera leaf extract by the appropriate extraction method. Indust Crops Products, 44, 566-71. https://doi.org/10.1016/j.indcrop.2012.09.021
  38. Vongsak B, Sithisarn P, Gritsanapan W (2013b). Bioactive contents and free radical scavenging activity of Moringa oleifera leaf extract under different storage conditions. Indust Crops Products, 49, 419-21. https://doi.org/10.1016/j.indcrop.2013.05.018
  39. World Cancer Report (WCR) (2008). International agency for research on cancer. Retrieved 2011-02-26.

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