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Cytotoxicity Assessment of Six Different Extracts of Abelia triflora leaves on A-549 Human Lung Adenocarcinoma Cells

  • Al-Taweel, Areej Mohammad (Department of Pharmacognosy, College of Pharmacy, King Saud University) ;
  • Perveen, Shagufta (Department of Pharmacognosy, College of Pharmacy, King Saud University) ;
  • Fawzy, Ghada Ahmed (Department of Pharmacognosy, College of Pharmacy, King Saud University) ;
  • Ibrahim, Taghreed Abdou (Department of Pharmacognosy, College of Pharmacy, King Saud University) ;
  • Khan, Afsar (Department of Chemistry, COMSATS Institute of Information Technology) ;
  • Mehmood, Rashad (Department of Conservation Studies, Hazara University)
  • Published : 2015.06.26

Abstract

The present investigation was designed to assess the anticancer activity of six different leaf extracts (ethyl acetate, methanol, chloroform, petroleum ether, n-butanol, and water soluble) of Abelia triflora on A-549 human lung adenocarcinoma epithelial cells. A-549 cells were exposed to $10-1000{\mu}g/ml$ concentrations of the leaf extracts of A. triflorafor 24 h and then percentage cell viability was assessed by 3-(4,5-dimethylthiazol-2yl)-2,5-biphenyl tetrazolium bromide (MTT) assay. The results showed that leaf extracts of A. triflora significantly reduced the viability of A-549 cells in a concentration-dependent manner. Decrease was recorded as 31% with ethyl acetate, 36% with methanol, 46% with chloroform, 54% with petroleum ether, 62% with n-butanol, and 63% with water soluble extracts at $1000{\mu}g/ml$ each. Among the various plant extracts, ethyl acetate extract showed the highest decrease in the percentage cell viability, followed by methanol, chloroform, petroleum ether, n-butanol, and water soluble extracts. Our results demonstrated preliminary screening of anticancer activity of different soluble extracts of A. triflora extracts against A-549 cells, which can be further used for the development of a potential therapeutic anticancer agents.

Keywords

Abelia triflora;A-549 cell line;cytotoxicity;MTT Assay

Acknowledgement

Grant : Research Center of the Female Scientific and Medical Colleges

Supported by : King Saud University

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