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Peptide H reduces Il-6 expression in human breast cancer MDA-MB-231 cells

인간유방암 MDA-MB-231세포에서 peptide H에 의한 TNFα 발현 억제

  • Sung, Dae Il (Department of Biotechnology, The Research Institute for Basic Sciences, Hoseo University) ;
  • Park, Jameon (Department of Biotechnology, The Research Institute for Basic Sciences, Hoseo University) ;
  • Kang, Choong Kyung (Department of Nanobiotronics, Bioconvergence Lab, Hoseo University) ;
  • Kim, Han Bok (Department of Biotechnology, The Research Institute for Basic Sciences, Hoseo University)
  • 성대일 (호서대학교 생명공학과 기초과학연구소) ;
  • 박잠언 (호서대학교 생명공학과 기초과학연구소) ;
  • 강충경 (호서대학교 나노바이오트로닉스학과 바이오융합연구소) ;
  • 김한복 (호서대학교 생명공학과 기초과학연구소)
  • Received : 2015.07.28
  • Accepted : 2015.09.12
  • Published : 2015.09.30

Abstract

Chungkookjang (fermented soybeans) contains diverse peptides. Human breast cancer MDA-MB-231 cells were treated with peptide H derived from Chungkookjang, and $TNF{\alpha}$ expression in the cells was conspicuously repressed, suggesting peptide H's regulation of IL6 since IL6 is induced by $TNF{\alpha}$. The structure of peptide H was different from those of glucocorticoid and dexamethasone, suggesting different mechanisms of $TNF{\alpha}$ expression suppression. Peptide H which reduces $TNF{\alpha}$ expression can be developed as drugs for cancer, rheumatoid arthritis and Crohn's disease, after more investigation.

Keywords

breast cancer;inflammation;peptide;$TNF{\alpha}$

References

  1. Berman, H.M., Westbrook, J., Feng, Z., Gilliland, G., Bhat, T.N., Weissig, H., Shindyalov, I.N., and Bourne, P.E. 2000. The protein data bank. Nucleic Acids Res. 28, 235-242. https://doi.org/10.1093/nar/28.1.235
  2. Hwang, J.S., Yoo, H.J., Song, H.J., Kim, K.K., Chun, Y.J., Matsui, T., and Kim, H.B. 2011. Inflammation-related signaling pathways implicating $TGF{\beta}$ are revealed in the expression profiling of MCF7 cell treated with fermented soybean, Chungkookjang. Nutr. Cancer 63, 645-652. https://doi.org/10.1080/01635581.2011.551987
  3. Landskron, G., De la Fuente, M., Thuwajit, P., Thuwajit, C., and Hermoso, M.A. 2014. Chronic inflammation and cytokines in the tumor microenvironment. J. Immunol. Res. http://dx.doi.org/10.1155/2014/149185. https://doi.org/10.1155/2014/149185
  4. Lee, J.J., Lee, D.S., and Kim, H.B. 1999. Fermentation patterns of Chungkookjang and Kanjang by Bacillus licheniformis B1. Kor. J. Microbiol. 35, 296-301.
  5. Lee, W.H., Wu, H.M., Lee, C.G., Sung, D.I., Song, H.J., Matsui, T., Kim, H.B., and Kim, S.G. 2014. Specific oligopeptides in fermented soybean extract inhibit NF-${\kappa}B$-dependent iNOS and cytokine induction by toll like receptor ligands. J. Med. Food 17, 1239-1246. https://doi.org/10.1089/jmf.2013.3070
  6. Lin, H., Lin, T., Wu, M., Chiu, Y., Tang, C., Hour, M., Liou, H., Tu, H., Yang, R., and Fu, W. 2014. 5-Lipoxigenase inhibitors attenuate TNF-${\alpha}$-induced inflammation in human synovial fibroblasts. PLoS One 9, e107890. https://doi.org/10.1371/journal.pone.0107890
  7. Matsui, T., Yoo, H.J., Hwang, J.S., Lee, D.S., and Kim, H.B. 2004. Isolation of angiotensin-1-converting enzyme inhibitory peptide from Chungkookjang. Kor. J. Microbiol. 40, 355-358.
  8. McInners, I.B., Leung, B.P., Sturock, R.D., Field, M., and Liew, F.Y. 1997. Interleukin-15 mediates T cell-dependent regulation of tumor necrosis factor-${\alpha}$ production in rheumatoid arthritis. Nature Med. 3, 189-195. https://doi.org/10.1038/nm0297-189
  9. Ndlovu, M.N., Lint, C.V., Wesmael, K.V., Callebert, P., Chalbos, D., Haegeman, G., and Berghe, W.V. 2009. Hyperactivated NF-${\kappa}B$ and AP-1 transcription factors promote highly accessible chromatin and constitutive transcription across the interleukin-6 gene promoter in metastatic breast cancer cells. Mol. Cell Biol. 29, 5488-5504. https://doi.org/10.1128/MCB.01657-08
  10. Sawada, M., Suzumura, A., and Marunouchi, T. 1992. TNF${\alpha}$ induces IL-6 production by astrocytes but not by microglia. Brain Res. 583, 296-299. https://doi.org/10.1016/S0006-8993(10)80037-X
  11. Somvanshi, R.K., Singh, A.K., Saxena, M., Mishra, B., and Dey, S. 2008. Development of novel peptide inhibitor of lipoxygenase based on biochemical and BlAcore evidences. Biochim. Biophys. Acta 1784, 1812-1817. https://doi.org/10.1016/j.bbapap.2008.07.004
  12. Sung, D.I., Park, J., and Kim, H.B. 2014. Peptide H reduces IL-6 expression in human breast cancer MDA-MB-231 cells. Kor. J. Microbiol. 50, 261-263. https://doi.org/10.7845/kjm.2014.4048
  13. Swantek, J.L., Cobb, M.H., and Geppert, T.D. 1997. Jun N-terminal kinase/stress-activated protein kinase (JNK/SAPK) is required for lipopolysaccharide stimulation of tumor necrosis factor alpha (TNF${\alpha}$) translation: glucocorticoids inhibit TNF${\alpha}$translation by blocking JNK/SAPK. Mol. Cell. Biol. 17, 6274-6282. https://doi.org/10.1128/MCB.17.11.6274
  14. Tanabe, K., Matsushima-Nishiwaki, R., Yamaguchi S., Iida, H., Dohi, S., and Kozawa, O. 2010. Mecanisms of tumor necrosis factor-alpha-induced interleukin-6 synthesis in glioma cells. J. Neuroinflam. 7, 16. https://doi.org/10.1186/1742-2094-7-16
  15. Trabuco, L.G., Lise, S., Petsalaki, E., and Russell, R.B. 2012. PepSite: prediction of peptide-binding sites from protein surfaces. Nucleic Acids Res. 40, 423-427. https://doi.org/10.1093/nar/gks398
  16. Van Dullemen, H.M., Deventer, S.J.H., Homes, D.W., Bijl, H.A., Jansen, J., Tytgat, G.N.J., and Woody, J. 1995. Treatment of Crohn's disease with anti-tumer neucrosis factor chimeric monoclonal antibody (cA2). Gastroenterol. 109, 129-135. https://doi.org/10.1016/0016-5085(95)90277-5
  17. Wenzel, M., Chiriac, A.L., Otto, A., zweytick, D., May, C., Schumacher, C., Gust, R., Albada, B., Penkova, M., Kramer U., et al. 2014. Small cationic antimicrobial peptides delocalize peripheral membrane proteins. Proc. Natl. Acad. Sci. USA 111, 1409-1418. https://doi.org/10.1073/pnas.1323697111