Hepatoprotective effect of Bifidobacterium adolescentis SPM0212 on carbon tetrachloride induced hepatotoxicity

사염화탄소로 유도된 간 손상에 대한 비피도박테리움 어돌레센티스 SPM0212의 보호효과

  • Received : 2015.08.03
  • Accepted : 2015.09.22
  • Published : 2015.09.30


Probiotics are microbial food supplements or components of bacteria which have traditionally been added to dairy foods for extra health boost. Our aim was to evaluate the hepatoprotective effect of Bifidobacterium adolescentis SPM0212 as probiotics, which we previously found has potential anti-hepatitis B virus activity. The study was conducted using Wistar albino rats and probiotics were treated orally for 9 days consecutively and acute liver injury was induced by administration of carbon tetrachloride ($CCl_4$) on the 7th and 8th days. Liver damage was assessed by quantifying serum activities of glutamate oxaloacetate transaminase (SGOT) and glutamate pyruvate transaminase (SGPT), as well as by histopathological examination. B. adolescentis SPM0212 significantly prevented the elevation of SGOT and SGPT levels, and reduced the negative effect of $CCl_4$ on body and organ weights. Histopathological study revealed the livers of the carbon tetrachloride treated rats showed almost complete loss of normal hepatocyte architecture, but that rats treated with B. adolescentis SPM0212 showed minimal damage and normal hepatocyte architecture. Our results suggest that B. adolescentis SPM0212 be considered useful probiotics for protecting the liver from xenobiotics and hepatitis B virus, and as well as useful as a functional food for maintaining human health.


Bifidobacterium adolescentis;carbon tetrachloride;hepatoprotective effect;probiotics


Supported by : Sahmyook University


  1. Adawi, D., Kasravi, F.B., Molin, G., and Jeppsson, B. 1997. Effect of Lactobacillus supplementation with and without arginine on liver damage and bacterial translocation in an acute liver injury model in the Rat. Hepatology 25, 642-647.
  2. Han, S.Y., Huh, C.S., Ahn, Y.T., Lim, K.S., Baek, Y.J., and Kim, D.H. 2005a. Hepatoprotective effect of lactic acid bacteria. J. Microbiol. Biotechnol. 15, 887-890.
  3. Han, S.Y., Huh, C.S., Ahn, Y.T., Lim, K.S., Baek, Y.J., and Kim, D.H. 2005b. Hepatoprotective effect of lactic acid bacteria, inhibitors of $\beta$-glucuronidase production against intestinal microflora. Arch. Pharm. Res. 28, 325-329.
  4. Jain, M., Kapadia, R., Jadeja, N.R., Thounaojam, C.M., Ranjitsinh, V.D., and Mishra, S.H. 2011. Cytotoxicity evaluation and hepatoprotective potential of bioassay guided fractions from Feronia limmonia Linn leaf. Asian Pac. J. Trop. Biomed. 10, 443-447.
  5. Kataria, J., Li, N., Wynn, J.L., and Neu, J. 2009. Probiotic microbes: do they need to be alive to be beneficial?. Nutr. Rev. 67, 546-550.
  6. Kim, J.H., Kim, H.J., Son, J.H., Chun, H.N., Yang, J.O., Choi, S.J., Paek, N.S., Choi, G.H., and Kim, S.K. 2003. Effect of Lactobacillus fermentum MG590 on alcohol metabolism and liver function in rats. J. Microbiol. Biotechnol. 13, 919-925.
  7. Kim, Y., Lee, D., Kim, D., Cho, J., Yang, J., and Chung, M. 2008. Inhibition of proliferation in colon cancer cell lines and harmful enzyme activity of colon bacteria by Bifidobacterium adolescentis SPM0212. Arch. Pharm. Res. 31, 468-473.
  8. Kitada, M., Igarashi, K., Hirose, S., and Kitagawa, H. 1979. Inhibition by polyamines of lipid peroxide formation in rat liver microsomes. Biochem. Biophys. Res. Commun. 87, 388-394.
  9. Kumaravelu, P., Dakshinamoorthy, D.P., Subramaniam, S., Devaraj, H., and Devaraj, N.S. 1995. Effect of eugenol on drugmetabolizing enzymes of carbon tetrachloride-intoxicated rat liver. Biochem. Pharmacol. 49, 1703-1707.
  10. Lee, D.K., Kang, J.Y., Shin, H.S., Park, I.H., and Ha, N.J. 2013. Antiviral activity of Bifidobacterium adolescentis SPM0212 against Hepatitis B virus. Arch. Pharm. Res. 36, 1525-1532.
  11. Lin, C.C. and Huang, P.C. 2000. Antioxidant and hepatoprotective effects of Acathopanax senticosus. Phytother. Res. 14, 489-494.<489::AID-PTR656>3.0.CO;2-G
  12. Low, D., Thomas, N.W., and Fry, J.R. 1995. Lobar variation of carbon tetrachloride hepatotoxicity in the rat. Toxicol. Lett. 81, 1-4.
  13. Luna, L.G. 1968. Manual histology staining methods of armed forces institute of pathology, pp. 1-31. 3rd ed. McGraw-Hill, New York, USA.
  14. Martin, P. and Fiedman, L.S. 2012. Assessment of liver function and diagnostic studies, pp. 1-19. In Fiedman, L.S. and Keefe, E.B. (eds.), Handbook of liver diseases, 3rd ed. Churchill Livingstone, Philadelphia, USA.
  15. McCormack, S.A. and Johnson, L.R. 1991. Role of polyamines in gastrointestinal mucosal growth. Am. J. Physiol. 260, 795-806.
  16. Muriel, P. 2007. Some experimental models of liver damage, pp. 119-130. In Sahu, S.C. (ed.), Hepatotoxicity: from genomics to in vitro and in vivo models. John Wiley & Sons Ltd., Chichester, UK.
  17. Nallamilli, B.R., Kumar, C.P., Reddy, K.V., Prasanna, M.L., Maruthi, V., and Sucharita, P. 2013. Hepatoprotective activity of Cichorium intybus (Linn.) root extract against carbon tetrachloride induced hepatotoxicity in albino Wistar rats. Drug Invent. Today 5, 311-314.
  18. Nanji, A.A., Khettry, U., and Sadrzadeh, S.M.H. 1994. Lactobacillus feeding reduces endotoxemia and severity of experimental alcoholic liver (disease). Proc. Soc. Exp. Bio. Med. 205, 243-247.
  19. Nurrochmad, A., Margono, S.A., Sardjiman, Hakim, A.R., Ernawati, Kurniawati, E., and Fatmawati, E. 2013. Hepatoprotective and antioxidant activity of pentagamavunon-0 against carbon tetrachloride-induced hepatic injury in rats. Asian Pac. J. Trop. Biomed. 6, 438-442.
  20. Osman, N., Adawi, D., Ahrne, S., Jeppsson, B., and Molin, G. 2007. Endotoxin- and D-galactosamine induced liver injury improved by the administration of Lactobacillus, Bifidobacterium and blueberry. Dig. Liver Dis. 39, 849-856.
  21. Ou, C., Chiu, Y., Lin, S., Chang, Y., Huang, H., and Lin, M. 2012. Hepatoprotective effect of lactic acid bacteria in the attenuation of oxidative stress from tert-butyl hydroperoxide. J. Food Drug Anal. 20, 101-110.
  22. Parvez, S., Malik, K.A., Ah Kang, S., and Kim, H.Y. 2006. Probiotics and their fermented food products are beneficial for health. J. Appl. Microbiol. 100, 1171-1185.
  23. Plaza-Diaz, J., Gomez-Llorente, C., Abadia-Molina, F., Saez-Lara, M.J., Cpmpana-Martin, L., Munoz-Quezada, S., Romero, F., Gil, A., and Fontana, L. 2014. Effects of Lactobacillus paracasei CNCM I-4034, Bifidobacterium breve CNCm I-4035 and Lactobacillus rhamnosus CNCM I-4036 on hepatic steatosis in Zucker rats. PLoS One 9, e98401.
  24. Recknagel, R.O., Glende Jr., E.A., Dolak, J.A., and Waller, R.L. 1989. Mechanisms of carbon tetrachloride toxicity. Pharmacal. Ther. 43, 139-154.
  25. Reid, G. 2005. The importance of guidelines in the development and application of probiotics. Curr. Pharm. Des. 11, 11-16.
  26. Samal, P.K. and Dangi, J.S. 2014. Isolation, preliminary characterization and hepatoprotective activity of polysaccharides from Tamarindus indica L. Carbohydr. Polym. 102, 1-7.
  27. Solga, S.F. 2003. Probiotics can treat hepatic encephalopathy. Med. Hypotheses 61, 307-313.
  28. Teselkin, Y.O., Babenkova, I.V., Kolhir, V.K., Baginskaya, A.I., Tjukavkina, N.A., Kolesnik, Y.A., Selivanova, I.A., and Eichholz, A.A. 2000. Dihydroquercetin as a means of antioxidative defence in rats with tetrachloromethane hepatitis. Phytother. Res. 14, 160-162.<160::AID-PTR555>3.0.CO;2-Y
  29. Uemitsu, N. and Nakayoshi, H. 1984. Evaluation of liver weight changes following a single oral administration of carbon tetrachloride in rats. Toxicol. Appl. Pharmacol. 75, 1-7.
  30. Wang, G.F., Shi, L.P., Ren, Y.D., Liu, Q.F., Liu, H.F., Zhang, R.J., Li, Z., Zhu, F.H., He, P.L., Tang, W., et al. 2009. Anti-hepatitis B virus activity of chlorogenic acid, quinic acid and caffeic acid in vivo and in vitro. Antiviral Res. 83, 186-190.
  31. Wang, Y., Li, Y., Xie, J., Zhang, Y., Wang, J., Sun, X., and Zhang, H. 2013. Protective effects of probiotic Lactobacillus casei Zhang against endotoxin- and D-galactosamine-induced liver injury in rats via anti-oxidative and anti-inflammatory capacities. Int. Immunopharmacol. 15, 30-37.
  32. Xing, H.C., Li, L.J., Xu, K.J., Shen, T., Chen, Y.B., Sheng, J.F., Chen, Y., Fu, S.Z., Chen, C.L., Wang, J.G., et al. 2006. Protective role of supplement with foreign Bifidobacterium and Lactobacillus in experimental hepatic ischemia-reperfusion injury. J. Gastroenterol. Hepatol. 21, 647-656.
  33. Yun, J.H., Kim, Y.A., Chung, M.J., Kang, B.Y., and Ha, N.J. 2007. Hepatoprotective and anti-fatigue effects of lactic acid bacteria (Lactobacillus acidophilus, Bifidobacterium bifidum and Streptococcus thermophilus). J. Toxicol. Pub. Health 23, 11-17.
  34. Zedan, Z.K. 2011. Hepatoprotective effect of Lactobacillus plantarum against Salmonella typhimurium in mice. J. Al-Nahrain Univ. 14, 126-131.
  35. Zhao, H.Y., Wang, H.J., Lu, Z., and Xu, S.Z. 2004. Intestinal microflora in patients with liver cirrhosis. Chin. J. Dig. Dis. 5, 64-67.