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Characterization of two β-mannanases from Cellulosimicrobium sp. YB-43

Cellulosimicrobium sp. YB-43에 의해 생산되는 2종류 β-mannanase의 특성분석

  • Yoon, Ki-Hong (Food Science and Biotechnology Major, Woosong University)
  • 윤기홍 (우송대학교 바이오식품과학전공)
  • Received : 2015.08.05
  • Accepted : 2015.09.04
  • Published : 2015.09.30

Abstract

A bacterial strain producing extracellular mannanases was isolated from soil of chestnut tree farm located in Gongju city of Korea by enrichment culture using Avicel as a carbon source. 16S rDNA sequence of the isolate YB-43 was highly homologous to those of genus Cellulosimicrobium strains with sequence similarities of above 99.6%. Mannanase productivity was significantly increased when the Cellulosimicrobium sp. YB-43 was grown in the presence of locust bean gum (LBG) or konjac. The mannanases were partially purified to be mannanase A (ManA) and mannanase C (ManC) by DEAE-Sepharose column and Q-Sepharose column chromatography from the culture filtrate of Cellulosimicrobium sp. YB-43 grown in LB medium supplemented with 0.7% LBG for 24 h. The partially purified ManA showed the highest activity at $55^{\circ}C$ and pH 6.5, while ManC activity was optimal at $65^{\circ}C$ and pH 7.5. ManA was stable up to $40^{\circ}C$ for 1 h, but ManC activity decreased significantly even after 1 h at $20^{\circ}C$. ManA and ManC showed difference from each other according to their substrate specificities and predominant products resulting from the mannanase hydrolysis for mannooligosaccharides. As a result, Cellulosimicrobium sp. YB-43 was found to produce two different kinds of mannanases.

Keywords

Cellulosimicrobium sp. YB-43;mannanases;productivity;property

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