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Temperature dependent 2,3-dihydroxybenzoic acid production in Acinetobacter sp. B-W

Acinetobacter sp. B-W의 온도 의존적 2,3-dihydroxybenzoic acid 생산

  • Kim, Kyoung-Ja (Department of life Science and Biotechnology, College of Natural Science, Soonchunhyang University) ;
  • Lee, Jae-Hun (Department of life Science and Biotechnology, College of Natural Science, Soonchunhyang University) ;
  • Yang, Yong-Joon (Department of Plant and Food Science, Sangmyung University)
  • 김경자 (순천향대 생명시스템학과) ;
  • 이재훈 (순천향대 생명시스템학과) ;
  • 양용준 (상명대학교 식물식품공학과)
  • Received : 2015.08.06
  • Accepted : 2015.08.26
  • Published : 2015.09.30

Abstract

A soil microorganism producing iron chelator (siderophore) under low iron stress (up to $2{\mu}M$ of iron) was identified as Acinetobacter sp. B-W by 16S rDNA sequence analysis, biochemical-, physiological tests and morphological analysis using electron microscope. Catechol nature of siderophore was detected by Arnow test. Although optimal cell growth was identified at $36^{\circ}C$ in iron-limited media, significant quantities of siderophore were produced only at $28^{\circ}C$. Biosynthesis of siderophore was strongly inhibited by growth at $36^{\circ}C$. Production of siderophore was completely inhibited by $10{\mu}M\;FeCl_3$. Iron chelator produced from Acinetobacter sp. B-W was purified from supernatant using butanol extraction, Sephadex LH-20 column chromatography and HPLC. Purified sideropore was identified as 2,3-dihydroxybenzoic acid by HPLC, TLC and IR analysis.

Keywords

Acinetobacter sp.;catechol type;2,3-dihydroxybenzoic acid;siderophore;temperature dependent

Acknowledgement

Supported by : Soonchunhyang University

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