- Volume 51 Issue 3
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Assessment of cell adhesion, cell surface hydrophobicity, autoaggregation, and lipopolysaccharide-binding properties of live and heat-killed Lactobacillus acidophilus CBT LA1
락토바실러스 아시도필러스 CBT LA1 생균과 사균체의 세포부착력, 자가응집력, 소수성 상호작용력, LPS 결합력에 대한 평가
- Shin, Joo-Hyun (R&D Center, Cell Biotech, Co., Ltd.) ;
- Lee, Joong-Su (R&D Center, Cell Biotech, Co., Ltd.) ;
- Seo, Jae-Gu (R&D Center, Cell Biotech, Co., Ltd.)
- Received : 2015.07.28
- Accepted : 2015.08.18
- Published : 2015.09.30
Although studies on probiotics have been performed mostly with viable microbes, the beneficial functions of dead or heat-killed form of probiotic strains have also been examined. In this study, live and heat-killed forms of Lactobacillus acidophilus CBT LA1 were investigated in vitro and in vivo to evaluate the properties necessary for gut barrier protection. Cell surface hydrophobicity (CSH), autoaggregation (AA), cell adhesion, and lipopolysaccharide (LPS)-binding properties were evaluated. In addition, the suppressive effect on LPS-induced interleukin (IL)-8 expression was investigated in HT-29 cells. To identify optimal conditions for CBT LA1 to adhere to HT-29 cells, CBT LA1 cells were heat-treated at 80, 85, 90, 95, 100, or
Lactobacillus acidophilus CBT LA1;cell adhesion;heat-killed bacteria;LPS-binding property;pharmabiotic;probiotics
Grant : 식중독균 제어를 위한 항생제 동시투여 가능 사균체 개발
- Barbara, G., De Giorgio, R., Stanghellini, V., Cremon, C., and Corinaldesi, R. 2002. A role for inflammation in irritable bowel syndrome? Gut 51 (Suppl 1), i41-i44. https://doi.org/10.1136/gut.51.suppl_1.i41
- Boris, J.E., Suarez, S., and Barbes, C. 1997. Characterization of the aggregation promoting factor from Lactobacillus gasseri, a vaginal isolate. J. Appl. Microbiol. 83, 413-420. https://doi.org/10.1046/j.1365-2672.1997.00250.x
- Cani, P.D., Osto, M., Geurts, L., and Everard, A. 2012. Involvement of gut microbiota in the development of low-grade inflammation and type 2 diabetes associated with obesity. Gut Microbes 3, 279-288. https://doi.org/10.4161/gmic.19625
- Chuang, L., Wu, K.G., Pai, C., Hsieh, P.S., Tsai, J.J., Yen, J.H., and Lin, M.Y. 2007. Heat-killed cells of lactobacilli skew the immune response toward T helper 1 polarization in mouse splenocytes and dendritic cell-treated T cells. J. Agric. Food Chem. 55, 11080-11086. https://doi.org/10.1021/jf071786o
- Coconnier, M.H., Bernet, M.F., Chauviere, G., and Servin, A.L. 1993. Adhering heat-killed human Lactobacillus acidophilus, strain LB, inhibits the process of pathogenicity of diarrhoeagenic bacteria in cultured human intestinal cells. J. Diarrhoeal Dis. Res. 11, 235-242.
- Cohen, J. 2002. The immunopathogenesis of sepsis. Nature 420, 885-891. https://doi.org/10.1038/nature01326
- Del Re, B., Sgorbati, B., Miglioli, B.M., and Palenzona, D. 2000. Adhesion, autoaggregation and hydrophobicity of 13 strains of Bifidobacterium longum. Lett. Appl. Microbiol. 31, 438-442. https://doi.org/10.1046/j.1365-2672.2000.00845.x
- Fourniat, J., Colomban, C., Linxe, C., and Karam, D. 1992. Heat-killed Lactobacillus acidophilus inhibits adhesion of Escherichia coli B41 to HeLa cells. Ann. Rech. Vet. 23, 361-370.
- Kiely, L.J. and Olson, N.F. 2000. The physicochemical surface characteristics of Lactobacillus casei. Food Microbiol. 17, 277-291. https://doi.org/10.1006/fmic.1999.0311
- Kiso, M., Takano, R., Sakabe, S., Katsura, H., Shinya, K., Uraki, R., Watanabe, S., Saito, H., Toba, M., Kohda, N., et al. 2013. Protective efficacy of orally administered, heat-killed Lactobacillus pentosus b240 against influenza A virus. Sci Rep. 3, 1563. https://doi.org/10.1038/srep01563
- Kwak, D.S., Jun, D.W., Seo, J.G., Chung, W.S., Park, S.E., Lee, K.N., Khalid-Saeed, W., Lee, H.L., Lee, O.Y., Yoon, B.C., et al. 2014. Short-term probiotic therapy alleviates small intestinal bacterial overgrowth, but does not improve intestinal permeability in chronic liver disease. Eur. J. Gastroenterol. Hepatol. 26, 1353-1359.
- Lim, N.K., Yoo, J.C., Park, T.R., and So, J.S. 1998. The relationship between cell surface hydrophobicity (CSH) and stress tolerance in Bifidobacterium spp. Food Sci. Biotechnol. 7, 66-70.
- Maeda, N., Nakamura, R., Hirose, Y., Murosaki, S., Yamamoto, Y., Kase, T., and Yoshikai, Y. 2009. Oral administration of heatkilled Lactobacillus plantarum L-137 enhances protection against influenza virus infection by stimulation of type I interferon production in mice. Int. Immunopharmacol. 9, 1122-1125. https://doi.org/10.1016/j.intimp.2009.04.015
- Naidu, A.S., Bidlack, W.R., and Clemens, R.A. 1999. Probiotic spectra of lactic acid bacteria (LAB). Cri. Rev. Food Sci. Nutr. 38, 13-126.
- Nilsen, E.M., Johansen, F.E., Jahnsen, F.L., Lundin, K.E., Scholz, T., Brandtzaeg, P., and Haraldsen, G. 1998. Cytokine profiles of cultured microvascular endothelial cells from the human intestine. Gut 42, 635-642. https://doi.org/10.1136/gut.42.5.635
- O'Hara, A.M. and Shanahan, F. 2007. Mechanisms of action of probiotics in intestinal diseases. Scientific World J. 7, 31-46. https://doi.org/10.1100/tsw.2007.26
- Park, M.S., Kim, M.J., and Ji, G.E. 2007. Assessment of lipopolysaccharide-binding activity of Bifidobacterium and its relationship with cell surface hydrophobicity, autoaggregation, and inhibition of interleukin-8 production. J. Microbiol. Biotechnol. 17, 1120-1126.
- Peng, G.C. and Hsu, C.H. 2005. The efficacy and safety of heat-killed Lactobacillus paracasei for treatment of perennial allergic rhinitis induced by house-dust mite. Pediatr. Allergy Immunol. 16, 433-438. https://doi.org/10.1111/j.1399-3038.2005.00284.x
- Ram, C. and Chander, H. 2003. Optimization of culture conditions of probiotic bifidobacteria for maximal adhesion to hexadecane. World J. Microbiol. Biotechnol. 19, 407-410. https://doi.org/10.1023/A:1023946702949
- Reid, G., McGroarty, J.A., Angotti, R., and Cook, R.L. 1988. Lactobacillus inhibitor production against Escherichia coli and coaggregation ability with uropathogens. Can. J. Microbiol. 34, 344-351. https://doi.org/10.1139/m88-063
- Rodes, L., Khan, A., Paul, A., Coussa-Charley, M., Marinescu, D., Tomaro-Duchesneau, C., Shao, W., Kahouli, I., and Prakash, S. 2013. Effect of probiotics Lactobacillus and Bifidobacterium on gut-derived lipopolysaccharides and inflammatory cytokines: an in vitro study using a human colonic microbiota model. J. Microbiol. Biotechnol. 23, 518-526. https://doi.org/10.4014/jmb.1205.05018
- Schillinger, U., Guigas, C., and Holzapfel, W.H. 2005. In vitro adherence and other properties of lactobacilli used in probiotic yoghurt-like products. Int. Dairy J. 15, 1289-1297. https://doi.org/10.1016/j.idairyj.2004.12.008
- Su, G.L. 2002. Lipopolysaccharides in liver injury: molecular mechanisms of Kupffer cell activation. Am. J. Physiol. Gastrointest. Liver Physiol. 283, G256-G265. https://doi.org/10.1152/ajpgi.00550.2001
- Tareb, R., Bernardeau, M., Gueguen, M., and Vernoux, J.P. 2013. In vitro characterization of aggregation and adhesion properties of viable and heat-killed forms of two probiotic Lactobacillus strains and interaction with foodborne zoonotic bacteria, especially Campylobacter jejuni. J. Med. Microbiol. 62, 637-649. https://doi.org/10.1099/jmm.0.049965-0
- Tuomola, E.M., Ouwehand, A.C., and Salminen, S.J. 2000. Chemical, physical and enzymatic pre-treatments of probiotic lactobacilli alter their adhesion to human intestinal mucus glycoproteins. Int. J. Food Microbiol. 60, 75-81. https://doi.org/10.1016/S0168-1605(00)00319-6
- Yoon, J.S., Sohn, W., Lee, O.Y., Lee, S.P., Lee, K.N., Jun, D.W., Lee, H.L., Yoon, B.C., Choi, H.S., Chung, W.S., et al. 2014. Effect of multispecies probiotics on irritable bowel syndrome: a randomized, double-blind, placebo-controlled trial. J. Gastroenterol. Hepatol. 29, 52-59. https://doi.org/10.1111/jgh.12322
- Isolation and Preliminary Screening of a Weissella confusa Strain from Giant Panda (Ailuropoda melanoleuca) pp.1867-1314, 2018, https://doi.org/10.1007/s12602-018-9402-2