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Microbial community structures in the ground bed cultivation of Lentinula edodes using oak sawdust

참나무 톱밥을 이용한 표고 지면재배 과정의 주요 미생물 군집 분석

  • Shin, Ji-Hye (Department of Microbiology, Chungbuk National University) ;
  • Yun, Seo-Yeon (Department of Microbiology, Chungbuk National University) ;
  • Nam, Ji-Hyun (Department of Environmental Engineering and Energy, Myongji University) ;
  • Koo, Chang-Duck (Department of Forest Science, Chungbuk National University) ;
  • Lee, Dong-Hun (Department of Microbiology, Chungbuk National University)
  • 신지혜 (충북대학교 미생물학과) ;
  • 윤서연 (충북대학교 미생물학과) ;
  • 남지현 (명지대학교 환경에너지공학과) ;
  • 구창덕 (충북대학교 산림학과) ;
  • 이동훈 (충북대학교 미생물학과)
  • Received : 2015.08.25
  • Accepted : 2015.09.22
  • Published : 2015.09.30

Abstract

Ground bed cultivation method for shiitake mushroom (Phogo; Lentinula edodes) has been advanced to use the oak-sawdust composted by microorganisms. This method has some advantages in saving the labors and improving productivity. The aim of this study is to analyze microbial community in oak sawdust and to provide the information for efficient cultivation of shiitake mushroom. In process of composting, the proportions of thermophiles increased from 10% to 80%. This results show the microbial community succession from mesophilic bacteria to thermophilic bacteria. T-RFLP analysis and nucleotide sequencing of 16S rRNA gene demonstrated that the change of dominant bacteria coupled with shifts in each stages. The family of Enterobacteriaceae as a mesophilic bacteria were dominant (100%) in oak sawdust sample, but Amycolatopsis (49.0%) and Saccharopolyspora (26.5%) as a thermophilic Actinomycetes were dominant in the last day of composting. In hyphal growth stage, Leuconostoc (psychrophilic bacteria) was dominant (75.0%). The composting process of sawdust is very important for growth of mushroom mycelium. Therefore, there is need for the further study for the Amycolatopsis as thermophilic Actinomycetes.

Keywords

Amycolatopsis;Lentinula edodes;composting;oak sawdust;shiitake mushroom

Acknowledgement

Supported by : 충북대학교

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