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Analysis of gut bacterial diversity and exploration of cellulose-degrading bacteria in xylophagous insects

목질섭식곤충의 장내 세균 다양성 분석 및 섬유소 분해균 탐색

  • Choi, Min-Young (Agricultural Microbiology Division, National Academy of Agricultural Science (NAAS), Rural Development Administration (RDA)) ;
  • Ahn, Jae-Hyung (Agricultural Microbiology Division, National Academy of Agricultural Science (NAAS), Rural Development Administration (RDA)) ;
  • Song, Jaekyeong (Agricultural Microbiology Division, National Academy of Agricultural Science (NAAS), Rural Development Administration (RDA)) ;
  • Kim, Seong-Hyun (Applied Entomology Division, NAAS, RDA) ;
  • Bae, Jin-Woo (Department of Biology, Kyung Hee University) ;
  • Weon, Hang-Yeon (Agricultural Microbiology Division, National Academy of Agricultural Science (NAAS), Rural Development Administration (RDA))
  • 최민영 (국립농업과학원 농업미생물과) ;
  • 안재형 (국립농업과학원 농업미생물과) ;
  • 송재경 (국립농업과학원 농업미생물과) ;
  • 김성현 (국립농업과학원 곤충산업과) ;
  • 배진우 (경희대학교 생물학과) ;
  • 원항연 (국립농업과학원 농업미생물과)
  • Received : 2015.05.15
  • Accepted : 2015.06.02
  • Published : 2015.09.30

Abstract

In this study, gut bacterial communities in xylophagous insects were analyzed using the pyrosequencing of 16S rRNA genes for their potential biotechnological applications in lignocelluloses degradation. The result showed that operational taxonomic units (OTUs), species richness and diversity index were higher in the hindgut than in the midgut of all insect samples analyzed. The dominant phyla or classes were Firmicutes (54.0%), Bacteroidetes (14.5%), ${\gamma}-Proteobacteria$ (12.3%) in all xylophagous insects except for Rhinotermitidae. The principal coordinates analysis (PCoA) showed that the bacterial community structure mostly clustered according to phylogeny of hosts rather than their habitats. In our study, the two carboxymethyl cellulose (CMC)-degrading isolates which showed the highest enzyme activity were most closely related to Bacillus toyonensis $BCT-7112^T$ and Lactococcus lactis subsp. hordniae $NCDO\;2181^T$, respectively. Cellulolytic enzyme activity analysis showed that ${\beta}-1,4-glucosidase$, ${\beta}-1,4-endoglucanase$ and ${\beta}-1,4-xylanase$ were higher in the hindgut of Cerambycidae. The results demonstrate that xylophagous insect guts harbor diverse gut bacteria, including valuable cellulolytic bacteria, which could be used for various biotechnological applications.

Keywords

cellulolytic bacteria;lignocellulose degradation;pyrosequencing;xylophagous insect

Acknowledgement

Supported by : 국립농업과학원

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