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Isolation and characterization of an Enterococcus faecalis bacteriophage

Enterococcus faecalis 특이적 박테리오파지의 분리와 특성규명

  • Kang, Hee-Young (Department of Microbiology, Kyungpook National University School of Medicine) ;
  • Kim, Shukho (Department of Microbiology, Kyungpook National University School of Medicine) ;
  • Kim, Jungmin (Department of Microbiology, Kyungpook National University School of Medicine)
  • 강희영 (경북대학교 의과대학 미생물학교실) ;
  • 김석호 (경북대학교 의과대학 미생물학교실) ;
  • 김정민 (경북대학교 의과대학 미생물학교실)
  • Received : 2015.07.08
  • Accepted : 2015.08.10
  • Published : 2015.09.30

Abstract

Enterococcus faecalis is a Gram-positive and facultative anaerobic bacterium that causes many hospital-acquired infections. Novel E. faecalis specific bacteriophage (phage) ECP3 that had been isolated from thirty-four environmental samples and characterized phenotypically and genotypically. ECP3 phage belongs to the family Myoviridae with contractile tail and lysed E. faecalis specifically but other bacteria including Enterococcus faecium. The genome was double-stranded linear DNA and its size was 145,518 bp comprising of 220 open reading frames. The GC content was 35.9%. The genome sequence showed 97% identity in 90% coverage region with Myoviridae phage PhiEF24C. ECP3 is the first E. faecalis-specific Myoviridae phage isolated in Korea which can be a promising antimicrobial agent against E. faecalis infections.

Enterococcus faecalis는 그람 양성의 조건 혐기성 세균으로 원내감염의 주요 원인균 중의 하나이다. E. faecalis에 특이적인 신규 박테리오파지 ECP3를 환경에서 분리하여 형태와 유전적 특성을 규명하였다. ECP3 파지는 형태적으로 수축성 꼬리를 갖는 미오비리대 과에 속하며 E. faecalis를 특이적으로 사멸하지만 Enterococcus faecium를 포함한 다른 세균은 사멸시키지 않았다. ECP3 파지의 게놈은 이중 선형 DNA로 GC 함량이 35.9%이었으며 크기가 145,518 bp이며 220개의 구조유전자로 구성되어 있었다. ECP3 게놈과 가장 유사한 염기서열은 미오비리대 PhiEF24C 파지이었으며 전체 게놈 90%의 영역에서 97%의 유사도를 보였다. ECP3 파지는 한국에서 처음 분리된 E. faecalis 미오비리대 파지이며 E. faecalis 감염에 대한 신규 항균제로서 유망하다.

Keywords

Acknowledgement

Supported by : 보건복지부

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