DOI QR코드

DOI QR Code

Isolation and characterization of an Enterococcus faecalis bacteriophage

Enterococcus faecalis 특이적 박테리오파지의 분리와 특성규명

  • Kang, Hee-Young (Department of Microbiology, Kyungpook National University School of Medicine) ;
  • Kim, Shukho (Department of Microbiology, Kyungpook National University School of Medicine) ;
  • Kim, Jungmin (Department of Microbiology, Kyungpook National University School of Medicine)
  • 강희영 (경북대학교 의과대학 미생물학교실) ;
  • 김석호 (경북대학교 의과대학 미생물학교실) ;
  • 김정민 (경북대학교 의과대학 미생물학교실)
  • Received : 2015.07.08
  • Accepted : 2015.08.10
  • Published : 2015.09.30

Abstract

Enterococcus faecalis is a Gram-positive and facultative anaerobic bacterium that causes many hospital-acquired infections. Novel E. faecalis specific bacteriophage (phage) ECP3 that had been isolated from thirty-four environmental samples and characterized phenotypically and genotypically. ECP3 phage belongs to the family Myoviridae with contractile tail and lysed E. faecalis specifically but other bacteria including Enterococcus faecium. The genome was double-stranded linear DNA and its size was 145,518 bp comprising of 220 open reading frames. The GC content was 35.9%. The genome sequence showed 97% identity in 90% coverage region with Myoviridae phage PhiEF24C. ECP3 is the first E. faecalis-specific Myoviridae phage isolated in Korea which can be a promising antimicrobial agent against E. faecalis infections.

Keywords

Enterococcus faecalis;bacteriophage;genome sequence;host specificity

Acknowledgement

Supported by : 보건복지부

References

  1. Aarestrup, F.M., Agerso, Y., Gerner-Smidt, P., Madsen, M., and Jensen, L.B. 2000. Comparison of antimicrobial resistance phenotypes and resistance genes in Enterococcus faecalis and Enterococcus faecium from humans in the community, broilers, and pigs in Denmark. Diagn. Microbiol. Infect. Dis. 37, 127-137. https://doi.org/10.1016/S0732-8893(00)00130-9
  2. Fischetti, V.A. 2008. Bacteriophage lysins as effective antibacterials. Curr. Opin. Microbiol. 11, 393-400. https://doi.org/10.1016/j.mib.2008.09.012
  3. Fischetti, V.A. 2010. Bacteriophage endolysins: a novel anti-infective to control Gram-positive pathogens. Int. J. Med. Microbiol. 300, 357-362. https://doi.org/10.1016/j.ijmm.2010.04.002
  4. Gutierrez, D., Martin-Platero, A.M., Rodriguez, A., Martinez-Bueno, M., Garcia, P., and Martinez, B. 2011. Typing of bacteriophages by randomly amplified polymorphic DNA (RAPD)-PCR to assess genetic diversity. FEMS Microbiol. Lett. 322, 90-97. https://doi.org/10.1111/j.1574-6968.2011.02342.x
  5. Guzman, C.A., Pruzzo, C., LiPira, G., and Calegari, L. 1989. Role of adherence in pathogenesis of Enterococcus faecalis urinary tract infection and endocarditis. Infect. Immun. 57, 1834-1838.
  6. Kim, S., Kim, M.J., Kang, H.Y., Seol, S.Y., Cho, D.T., and Kim, J. 2010. A simple colorimetric method for testing antimicrobial susceptibility of biofilmed bacteria. J. Microbiol. 48, 709-711. https://doi.org/10.1007/s12275-010-0299-z
  7. Kim, S., Rahman, M., Seol, S.Y., Yoon, S.S., and Kim, J. 2012. Pseudomonas aeruginosa bacteriophage PA1O requires type IV pili for infection and shows broad bactericidal and biofilm removal activities. Appl. Environ. Microbiol. 78, 6380-6385. https://doi.org/10.1128/AEM.00648-12
  8. Nallapareddy, S.R., Singh, K.V., Sillanpaa, J., Garsin, D.A., Hook, M., Erlandsen, S.L., and Murray, B.E. 2006. Endocarditis and biofilm-associated pili of Enterococcus faecalis. J. Clin. Invest. 116, 2799-2807. https://doi.org/10.1172/JCI29021
  9. Rahman, M., Kim, S., Kim, S.M., Seol, S.Y., and Kim, J. 2011. Characterization of induced Staphylococcus aureus bacteriophage SAP-26 and its anti-biofilm activity with rifampicin. Biofouling 27, 1087-1093. https://doi.org/10.1080/08927014.2011.631169
  10. Rinkinen, M., Jalava, K., Westermarck, E., Salminen, S., and Ouwehand, A.C. 2003. Interaction between probiotic lactic acid bacteria and canine enteric pathogens: a risk factor for intestinal Enterococcus faecium colonization?. Vet. Microbiol. 92, 111-119. https://doi.org/10.1016/S0378-1135(02)00356-5
  11. Ruiz-Garbajosa, P., Bonten, M.J., Robinson, D.A., Top, J., Nallapareddy, S.R., Torres, C., Coque, T.M., Cantón, R., Baquero, F., Murray, B.E., et al. 2006. Multilocus sequence typing scheme for Enterococcus faecalis reveals hospital-adapted genetic complexes in a background of high rates of recombination. J. Clin. Microbiol. 44, 2220-2228. https://doi.org/10.1128/JCM.02596-05
  12. Stuart, C.H., Schwartz, S.A., Beeson, T.J., and Owatz, C.B. 2006. Enterococcus faecalis: its role in root canal treatment failure and current concepts in retreatment. J. Endod. 32, 93-98.
  13. Sulakvelidze, A., Alavidze Z., and Morris Jr., J.G. 2001. Bacteriophage therapy. Antimicrob. Agents Chemother. 45, 649-659. https://doi.org/10.1128/AAC.45.3.649-659.2001
  14. Toledo-Arana, A., Valle, J., Solano, C., Arrizubieta, M.J., Cucarella, C., Lamata, M., Amorena, B., Leiva, J., Penades, J.R., and Lasa, I. 2001. The enterococcal surface protein, Esp, is involved in Enterococcus faecalis biofilm formation. Appl. Environ. Microbiol. 67, 4538-4545. https://doi.org/10.1128/AEM.67.10.4538-4545.2001
  15. Uchiyama, J., Rashel, M., Maeda, Y., Takemura, I., Sugihara, S., Akechi, K., Muraoka, A., Wakiguchi, H., and Matsuzaki, S. 2008. Isolation and characterization of a novel Enterococcus faecalis bacteriophage ${\varphi}EF24C$ as a therapeutic candidate. FEMS Microbiol. Lett. 278, 200-206. https://doi.org/10.1111/j.1574-6968.2007.00996.x
  16. Zhang, W., Mi, Z., Yin, X., Fan, H., An, X., Zhang, Z., Chen, J., and Tong, Y. 2013. Characterization of Enterococcus faecalis phage IME-EF1 and its endolysin. PLoS One 8, e80435. https://doi.org/10.1371/journal.pone.0080435