Isolation and characterization of an Enterococcus faecalis bacteriophage

Enterococcus faecalis 특이적 박테리오파지의 분리와 특성규명

  • Kang, Hee-Young (Department of Microbiology, Kyungpook National University School of Medicine) ;
  • Kim, Shukho (Department of Microbiology, Kyungpook National University School of Medicine) ;
  • Kim, Jungmin (Department of Microbiology, Kyungpook National University School of Medicine)
  • 강희영 (경북대학교 의과대학 미생물학교실) ;
  • 김석호 (경북대학교 의과대학 미생물학교실) ;
  • 김정민 (경북대학교 의과대학 미생물학교실)
  • Received : 2015.07.08
  • Accepted : 2015.08.10
  • Published : 2015.09.30


Enterococcus faecalis is a Gram-positive and facultative anaerobic bacterium that causes many hospital-acquired infections. Novel E. faecalis specific bacteriophage (phage) ECP3 that had been isolated from thirty-four environmental samples and characterized phenotypically and genotypically. ECP3 phage belongs to the family Myoviridae with contractile tail and lysed E. faecalis specifically but other bacteria including Enterococcus faecium. The genome was double-stranded linear DNA and its size was 145,518 bp comprising of 220 open reading frames. The GC content was 35.9%. The genome sequence showed 97% identity in 90% coverage region with Myoviridae phage PhiEF24C. ECP3 is the first E. faecalis-specific Myoviridae phage isolated in Korea which can be a promising antimicrobial agent against E. faecalis infections.


Enterococcus faecalis;bacteriophage;genome sequence;host specificity


Supported by : 보건복지부


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