DOI QR코드

DOI QR Code

Amino-terminal arginylation as a degradation signal for selective autophagy

  • Cha-Molstad, Hyunjoo (World Class Institute, Korea Research Institute of Bioscience and Biotechnology) ;
  • Kwon, Yong Tae (Protein Metabolism Medical Research Center and Department of Biomedical Sciences, College of Medicine, Seoul National University) ;
  • Kim, Bo Yeon (World Class Institute, Korea Research Institute of Bioscience and Biotechnology)
  • Received : 2015.08.24
  • Published : 2015.06.30

Abstract

The ubiquitin-proteasome system and the autophagy lysosome system are the two major protein degradation machineries in eukaryotic cells. These two systems coordinate the removal of unwanted intracellular materials, but the mechanism by which they achieve this synchronization is largely unknown. The ubiquitination of substrates serves as a universal degradation signal for both systems. Our study revealed that the amino-terminal Arg, a canonical N-degron in the ubiquitin-proteasome system, also acts as a degradation signal in autophagy. We showed that many ER residents, such as BiP, contain evolutionally conserved arginylation permissive pro-N-degrons, and that certain inducers like dsDNA or proteasome inhibitors cause their translocation into the cytoplasm where they bind misfolded proteins and undergo amino-terminal arginylation by arginyl transferase 1 (ATE1). The amino-terminal Arg of BiP binds p62, which triggers p62 oligomerization and enhances p62-LC3 interaction, thereby stimulating autophagic delivery and degradation of misfolded proteins, promoting cell survival. This study reveals a novel ubiquitin-independent mechanism for the selective autophagy pathway, and provides an insight into how these two major protein degradation pathways communicate in cells to dispose the unwanted proteins. [BMB Reports 2015; 48(9): 487-488]

Keywords

ATE1;p62;Autophagy

Cited by

  1. Titin, a Central Mediator for Hypertrophic Signaling, Exercise-Induced Mechanosignaling and Skeletal Muscle Remodeling vol.7, 2016, https://doi.org/10.3389/fphys.2016.00076
  2. Approaches for discovering novel bioactive small molecules targeting autophagy vol.12, pp.9, 2017, https://doi.org/10.1080/17460441.2017.1349751
  3. Autophagy-Related Deubiquitinating Enzymes Involved in Health and Disease vol.4, pp.4, 2015, https://doi.org/10.3390/cells4040596
  4. Structure biology of selective autophagy receptors vol.49, pp.2, 2016, https://doi.org/10.5483/BMBRep.2016.49.2.265
  5. Forkhead Box M1 positively regulates UBE2C and protects glioma cells from autophagic death vol.16, pp.18, 2017, https://doi.org/10.1080/15384101.2017.1356507
  6. Plant arginyltransferases (ATEs) vol.40, pp.1 suppl 1, 2017, https://doi.org/10.1590/1678-4685-gmb-2016-0084