DOI QR코드

DOI QR Code

Characterization of Selected Lactobacillus Strains for Use as Probiotics

  • Song, Minyu ;
  • Yun, Bohyun ;
  • Moon, Jae-Hak ;
  • Park, Dong-June ;
  • Lim, Kwangsei ;
  • Oh, Sejong
  • Received : 2015.07.14
  • Accepted : 2015.07.25
  • Published : 2015.08.31

Abstract

The aim of this study was to evaluate the functional properties of lactic acid bacteria from various sources and to identify strains for use as probiotics. Ten Lactobacillus strains were selected and their properties such as bile tolerance, acid resistance, cholesterol assimilation activity, and adherence to HT-29 cells were assessed to determine their potential as probiotics. Lactobacillus sp. JNU 8829, L. casei MB3, L. sakei MA9, L. sakei CH8, and L. acidophilus M23 were found to show full tolerance to the 0.3% bile acid. All strains without L. acidophilus M23 were the most acid-tolerant strains. After incubating the strains at pH 2.5 for 2 h, their viability decreased by 3 Log cells. Some strains survived at pH 2.5 in the presence of pepsin and 0.3% bile acid. Lactobacillus sp. JNU 8829, L. acidophilus KU41, L. acidophilus M23, L. fermentum NS2, L. plantarum M13, and L. plantarum NS3 were found to reduce cholesterol levels by >50% in vitro. In the adhesion assay, Lactobacillus sp. JNU 8829, L. casei MB3, L. sakei MA9, and L. sakei CH8 showed higher adhesion activities after 2 h of co-incubation with the intestinal cells. The results of this comprehensive analysis shows that this new probiotic strain named, Lactobacillus sp. JNU 8829 could be a promising candidate for dairy products.

Keywords

Lactobacillus;probiotics;acid and bile acid tolerances;cholesterol

References

  1. Ouwehand, A. C., Kirjavainen, P. V., Shortt, C., and Salminen, S. (1999) Probiotics: Mechanisms and established effects. Int. Dairy J. 9, 43-52. https://doi.org/10.1016/S0958-6946(99)00043-6
  2. Nguyen, T. D. T., Kang, J. H., and Lee, M. S. (2007) Characterization of Lactobacillus plantarum PH04, a potential probiotic bacterium with cholesterol-lowering effects. Int. J. Food Microbiol. 113, 358-361. https://doi.org/10.1016/j.ijfoodmicro.2006.08.015
  3. Nilakhe, S. and Sapre, V. (2015) Cholesterol assimilation by intestinal Lactobacilus acidophilus. Res. J. Chem. Environ. 19, 10-14.
  4. Noh, D. O., Kim, S. H., and Gilliland, S. E. (1997) Incorporation of cholesterol into the celluar membrane of Lactobacillus acidophilus ATCC 43121. J. Dairy Sci. 80, 3107-3113. https://doi.org/10.3168/jds.S0022-0302(97)76281-7
  5. Pereira, D. I. and Gibson, G. R. (2002) Cholesterol assimilation by lactic acid bacteria and bifidobacteria isolated from the human gut. Appl. Environ. Microbiol. 68, 4689-4693. https://doi.org/10.1128/AEM.68.9.4689-4693.2002
  6. Wang, J., Zhang, H., Chen, X., Chen, Y., Menghebilige, and Bao, Q. (2012) Selection of potential probiotic lactobacilli for cholesterol-lowering properties and their effect on cholesterol metabolism in rats fed a high-lipid diet. J. Dairy Sci. 95, 1645-1654. https://doi.org/10.3168/jds.2011-4768
  7. Rudel, L. L. and Morris, M. D. (1973) Determination of cholesterol using o-phthalaldehyde. J. Lipid Res. 14, 364-366
  8. Servin, A. L. and Coconnier, M. H. (2003) Adhesion of probiotic strains to the intestinal mucosa and interaction with pathogens. Best Pract. Res. Clin. Gastroenterol. 17, 741-754. https://doi.org/10.1016/S1521-6918(03)00052-0
  9. Usman, and Hosono, A. (1999) Bile tolerance, taurocholate deconjugation, and binding of cholesterol by Lactobacillus gasseri strains. J. Dairy Sci. 82, 243-248. https://doi.org/10.3168/jds.S0022-0302(99)75229-X
  10. Kim, S. J., Cho, S. Y., Kim, S. H., Song, O. J., Shin, I. S., Cha, D. S., and Park, H. J. (2008) Effect of microencapsulation on viability and other characteristics in Lactobacillus acidophilus ATCC 43121. LWT-Food Sci. Technol. 41, 493-500. https://doi.org/10.1016/j.lwt.2007.03.025
  11. Kumar, R., Grover, S., and Batish, V. K. (2012) Bile salt hydrolase activity screening of lactobacilli in vitro selection of indigenous Lactobacillus strains with potential bile salt hydrolysing and cholesterol-lowering ability. Probiotics Antimicro.Prot. 4, 162-172. https://doi.org/10.1007/s12602-012-9101-3
  12. Lehto, E. M. and Salminen, S. J. (1997) Inhibition of Salmonella typhimurium adhesion to Caco-2 cell cultures by Lactobacillus strain GG spent culture supernate: Only a pH effect? FEMS Immunol. Med. Microbiol. 18, 125-132. https://doi.org/10.1111/j.1574-695X.1997.tb01037.x
  13. Lim, S. M. (2014) Antimutagenicity activity of the putative probiotic strain Lactobacillus paracasei subsp. tolerans JG22 isolated from pepper leaves Jangajji. Food Sci. Biotechnol. 23, 141-150. https://doi.org/10.1007/s10068-014-0019-2
  14. Miyoshi, Y., Okada, S., Uchimura, T., and Satoh, E. (2006) A mucus adhesion promoting protein, MapA, mediates the adhesion of Lactobacillus reuteri to Caco-2 human intestinal epithelial cells. Biosci. Biotechnol. Biochem. 70, 1622-1628. https://doi.org/10.1271/bbb.50688
  15. Liong, M. T. and Shah, N. P. (2005) Acid and bile tolerance and cholesterol removal ability of lactobacilli strains. J. Dairy Sci. 88, 55-66. https://doi.org/10.3168/jds.S0022-0302(05)72662-X
  16. Maragkoudakis, P. A., Zoumpopoulou, G., Miaris, C., Kalantzopoulos, G., Pot, B., and Tsakalidou, E. (2006) Probiotic potential of Lactobacillus strains isolated from dairy products. Int. Dairy J. 16, 189-199. https://doi.org/10.1016/j.idairyj.2005.02.009
  17. Merrell, D. S. and Camilli, A. (2002) Acid tolerance of gastrointestinal pathogens. Curr. Opin. Microbiol. 5, 51-55. https://doi.org/10.1016/S1369-5274(02)00285-0
  18. Chou, L. S. and Weimer, B. (1999) Isolation and characterization of acid- and bile-tolerant isolates from strains of Lactobacillus acidophilus. J. Dairy Sci. 82, 23-31. https://doi.org/10.3168/jds.S0022-0302(99)75204-5
  19. du Toit, M., Franz, C. M., Dicks, L. M., Schillinger, U., Haberer, P., Warlies, B., Ahrensc, F., and Holzapfela, W. H. (1998) Characterisation and selection of probiotic lactobacilli for a preliminary minipig feeding trial and their effect on serum cholesterol levels, faeces pH and faeces moisture content. Int. J. Food Microbiol. 40, 93-104. https://doi.org/10.1016/S0168-1605(98)00024-5
  20. Dunne, C., O’Mahony, L., Murphy, L., Thornton, G., Morrissey, D., O’Halloran, S., Feeney, M., Flynn, S., Fitzgerald, G.,Daly, C., Kiely, B., O’Sullivan, G. C., Shanahan, F., and Collins, J. K. (2001) In vitro selection criteria for probiotic bacteria of human origin: Correlation with in vivo findings. Am. J. Clin. Nutr. 73, 386S-392S. https://doi.org/10.1093/ajcn/73.2.386s
  21. Hyronimus, B., Le Marrec, C., Sassi, A. H., and Deschamps, A. (2000) Acid and bile tolerance of spore-forming lactic acid bacteria. Int. J. Food Microbiol. 61, 193-197. https://doi.org/10.1016/S0168-1605(00)00366-4
  22. Jacobsen, C. N., Roesnfeldt Nielsen, V., Hayford, A. E., Moller, P. L., Michaelsen, K. F., Paerregaard, A., Sandström, B., Tvede, M., and Jacobsen, M. (1999) Screening of probioticac tivities of forty-seven strains of Lactobacillus spp. by in vitro techniques and evaluation of the colonization ability of five selected strains in humans. Appl. Environ. Microbiol. 65, 4949-4956.
  23. Kim, P. I., Jung, M. Y., Chang, Y. H., Kim, S., Kim, S. J., and Park, Y. H. (2007) Probiotic properties of Lactobacillus and Bifidobacterium strains isolated from porcine gastrointestinal tract. Appl. Microbiol. Biotechnol. 74, 1103-1111. https://doi.org/10.1007/s00253-006-0741-7
  24. Brashears, M. M., Gilliland, S. E., and Buck, L. M. (1998) Bile salt deconjugation and cholesterol removal from media by Lactobacillus casei. J. Dairy Sci. 81, 2103-2110. https://doi.org/10.3168/jds.S0022-0302(98)75785-6
  25. Buck, L. M. and Gilliland, S. E. (1994) Comparison of freshly isolated strains of Lactobacillus acidophilus of human intestinal origin for ability to assimilate cholesterol during growth. J. Dairy Sci. 77, 2925-2933. https://doi.org/10.3168/jds.S0022-0302(94)77233-7
  26. Charteris, W. P., Kelly, P. M., Morelli, L., and Collins, J. K. (1998) Antibiotic susceptibility of potentially probiotic Bifidobacterium isolates from the human gastrointestinal tract. Lett. Appl. Microbiol. 26, 333-337. https://doi.org/10.1046/j.1472-765X.1998.00342.x

Cited by

  1. Comparison of dairy desserts produced with a potentially probiotic mixed culture and dispersions obtained from Gracilaria birdiae and Gracilaria domingensis seaweeds used as thickening agents vol.8, pp.9, 2017, https://doi.org/10.1039/C7FO00776K
  2. The anti-allergic activity of Lactobacillus plantarum L67 and its application to yogurt vol.99, pp.12, 2016, https://doi.org/10.3168/jds.2016-11809
  3. Enhancement of Antioxidative and Intestinal Anti-inflammatory Activities of Glycated Milk Casein after Fermentation with Lactobacillus rhamnosus 4B15 vol.65, pp.23, 2017, https://doi.org/10.1021/acs.jafc.7b01339
  4. The advancement of probiotics research and its application in fish farming industries vol.115, 2017, https://doi.org/10.1016/j.rvsc.2017.01.016
  5. Lactobacillus plantarum L67 glycoprotein protects against cadmium chloride toxicity in RAW 264.7 cells vol.99, pp.3, 2016, https://doi.org/10.3168/jds.2015-10121
  6. Lactobacillus sakei: A Starter for Sausage Fermentation, a Protective Culture for Meat Products vol.5, pp.3, 2017, https://doi.org/10.3390/microorganisms5030056
  7. Dual function of Lactobacillus kefiri DH5 in preventing high-fat-diet-induced obesity: direct reduction of cholesterol and upregulation of PPAR-α in adipose tissue 2017, https://doi.org/10.1002/mnfr.201700252
  8. Influences of quorum-quenching probiotic bacteria on the gut microbial community and immune function in weaning pigs vol.89, pp.2, 2017, https://doi.org/10.1111/asj.12954
  9. Evaluation of adhesion properties of lactobacilli probiotic candidates vol.149, pp.5, 2018, https://doi.org/10.1007/s00706-017-2135-1

Acknowledgement

Grant : 고령친화형 특수용도식품(Silver Foods) 개발