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Anticancer Potential of Cratoxylum formosum Subsp. Pruniflorum (Kurz.) Gogel Extracts Against Cervical Cancer Cell Lines

  • Promraksa, Bundit (Department of Biochemistry, Medicine, Khon Kaen University) ;
  • Daduang, Jureerut (Centre for Research and Development of Medical Diagnostic Laboratories, Khon Kaen University) ;
  • Khampitak, Tueanjit (Department of Biochemistry, Medicine, Khon Kaen University) ;
  • Tavichakorntrakool, Ratree (Centre for Research and Development of Medical Diagnostic Laboratories, Khon Kaen University) ;
  • Koraneekit, Amonrat (Centre for Research and Development of Medical Diagnostic Laboratories, Khon Kaen University) ;
  • Palasap, Adisak (Centre for Research and Development of Medical Diagnostic Laboratories, Khon Kaen University) ;
  • Tangrassameeprasert, Roongpet (Department of Biochemistry, Medicine, Khon Kaen University) ;
  • Boonsiri, Patcharee (Department of Biochemistry, Medicine, Khon Kaen University)
  • Published : 2015.09.02

Abstract

Background: Most northeast Thai vegetables may play roles in human health by acting as antioxidant and anticancer agents. Recent study showed that Cratoxylum formosum subsp. pruniflorum (Kurz.) Gogel. (Teawdang) could inhibit growth of liver cancer cell lines. Cervical cancer, which has human papilloma virus as its main cause, is found at high incidence in Thailand. Due to increasing drug resistance, searches for potential anticancer compounds from natural source are required. Therefore, our purpose was to evaluate the cytotoxicity of Teawdang extracts in cervical cancer cell lines. Materials and Methods: Teawdang edible parts, purchased from Khon Kaen market during July-October 2013 was extracted with organic solvent. Phenolic profiles of crude hexane (CHE), ethyl acetate (CEE), methanol (CME) and water (CWE) extracts were performed by high performance liquid chromatographic (HPLC) techniques. Their cytotoxic effects on cervical cancer cells were investigated with HPV-non infected (C-33A) and HPV-infected (HeLa and SiHa) cell lines. Results: HPLC profiles showed that all crude extracts contained caffeine, ferulic acid and resveratrol. CME and CEE had high contents of gallic acid and quercetin. Catechin was found only in CWE. Cytotoxicity test showed that CEE had the lowest IC50 on HeLa ($143.18{\pm}13.35 {\mu}g/mL$) and SiHa cells ($106.45{\pm}15.73{\mu}g/mL$). C-33A cells were inhibited by CWE ($IC50=130.95{\pm}3.83{\mu}g/mL$). Conclusions: There were several phenolic compounds in Teawdang extracts which may have cytotoxic effects on cervical cancer cell lines. Investigation of these bioactive compounds as new sources of anticancer agents is recommended.

References

  1. Boonsri S, Karalai C, Ponglimanont C, et al (2006). Antibacterial and cytotoxic xanthones from the roots of Cratoxylum formosum. Phytochemistry, 67, 723-7. https://doi.org/10.1016/j.phytochem.2006.01.007
  2. Britten RA, Liu D, Tessier A, et al (2000). ERCC1 expression as a molecular marker of cisplatin resistance in human cervical tumor cells. Int J Cancer, 89, 453-7. https://doi.org/10.1002/1097-0215(20000920)89:5<453::AID-IJC9>3.0.CO;2-E
  3. Daduang J, Vichitphan S, Daduang S, et al (2011). High phenolics and antioxidants of some tropical vegetables related to antibacterial and anticancer activities. Afr J Pharm Pharmacol, 5, 608-15. https://doi.org/10.5897/AJPP10.243
  4. Daneshfar A, Ghaziaskar HS, Homayoun N (2008). Solubility of gallic acid in methanol, ethanol, water, and ethyl acetate. J Chem Eng Data, 53, 776-8. https://doi.org/10.1021/je700633w
  5. Di Domenico F, Foppoli C, Coccia R, et al (2012). Antioxidants in cervical cancer: chemopreventive and chemotherapeutic effects of polyphenols. Biochim Biophys Acta, 1822, 737-47. https://doi.org/10.1016/j.bbadis.2011.10.005
  6. Dung TT, Lee J, Kim E, et al (2015). Anti-inflammatory activities of Gouania leptostachya methanol extract and its constituent resveratrol. Phytother Res, 29, 381-92. https://doi.org/10.1002/ptr.5262
  7. Ferlay J, Soerjomataram I, Ervik M, et al (2013). GLOBOCAN 2008 V1.2. GLOBOCAN 2012 v1.0, Cancer Incidence and Mortality Worldwide: IARC CancerBase No. 11 (Internet). globocan.iarc.fr.
  8. Khuhaprema T, Srivatanakul P, Sriplung H, et al (2007). Cancer in Thailand: Vol. VII, 2007-2009. Bangkok: Bangkok Medical Publisher.
  9. Lee JH, Cho S, Paik HD, et al (2014). Investigation on antibacterial and antioxidant activities, phenolic and flavonoid contents of some Thai edible plants as an alternative for antibiotics. Asian-Australas J Anim Sci, 27, 1461-8. https://doi.org/10.5713/ajas.2013.13629
  10. Machana S, Weerapreeyakul N, Barusrux S, et al (2011). Cytotoxic and apoptotic effects of six herbal plants against the human hepatocarcinoma (HepG2) cell line. Chin Med, 6, 39. https://doi.org/10.1186/1749-8546-6-39
  11. Maisuthisakul P, Pongsawatmanit R, Gordon MH (2006). Antioxidant properties of Teaw (Cratoxylum formosum Dyer) extract in soybean oil and emulsions. J Agric Food Chem, 54, 2719-25. https://doi.org/10.1021/jf052396+
  12. Maisuthisakul P, Pongsawatmanit R, Gordon MH (2007). Characterization of the phytochemicals and antioxidant properties of extracts from Teaw (Cratoxylum formosum Dyer). Food Chem, 100, 1620-9. https://doi.org/10.1016/j.foodchem.2005.12.044
  13. Nonpunya A, Weerapreeyakul N, Barusrux S (2014). Cratoxylum formosum (Jack) Dyer ssp. pruniflorum (Kurz) Gogel. (Hong ya mu) extract induces apoptosis in human hepatocellular carcinoma HepG2 cells through caspase-dependent pathways. Chin Med, 9, 12. https://doi.org/10.1186/1749-8546-9-12
  14. Palasap A, Limpaiboon T, Boonsiri P, et al (2014). Cytotoxic efects of phytophenolics from Caesalpinia mimosoides Lamk on cervical carcinoma cell lines through an apoptotic pathway. Asian Pac J Cancer Prev, 15, 449-54. https://doi.org/10.7314/APJCP.2014.15.1.449
  15. Romagnolo DF, Selmin OI (2012). Flavonoids and cancer prevention: a review of the evidence. J Nutr Gerontol Geriatr, 31, 206-38. https://doi.org/10.1080/21551197.2012.702534
  16. Sak K (2012). Chemotherapy and dietary phytochemical agents. Chemother Res Pract, 2012, 282570.
  17. Saxena A, Yashar C, Taylor DD, et al (2005). Cellular response to chemotherapy and radiation in cervical cancer. Am J Obstet Gynecol, 192, 1399-403. https://doi.org/10.1016/j.ajog.2004.12.045
  18. Shen DW, Pouliot LM, Hall MD, et al (2012). Cisplatin resistance: a cellular self-defense mechanism resulting from multiple epigenetic and genetic changes. Pharmacol Rev, 64, 706-21. https://doi.org/10.1124/pr.111.005637
  19. Siriwatanametanon N, Fiebich BL, Efferth T, et al (2010). Traditionally used Thai medicinal plants: in vitro antiinflammatory, anticancer and antioxidant activities. J Ethnopharmacol, 130, 196-207. https://doi.org/10.1016/j.jep.2010.04.036
  20. Stewart P, Boonsiri P, Puthong S, et al (2013). Antioxidant activity and ultrastructural changes in gastric cancer cell lines induced by Northeastern Thai edible folk plant extracts. BMC Complement Altern Med, 13, 60. https://doi.org/10.1186/1472-6882-13-60
  21. Sun C, Brown AJ, Jhingran A, et al (2014). Patient preferences for side effects associated with cervical cancer treatment. Int J Gynecol Cancer, 24, 1077-84. https://doi.org/10.1097/IGC.0000000000000149
  22. Vidya PR, Senthil MR, Maitreyi S, et al (2010). The flavonoid quercetin induces cell cycle arrest and mitochondriamediated apoptosis in human cervical cancer (HeLa) cells through p53 induction and NF-kappaB inhibition. Eur J Pharmacol, 649, 84-91. https://doi.org/10.1016/j.ejphar.2010.09.020
  23. Waiyaput W, Payungporn S, Issara-Amphorn J, et al (2012). Inhibitory effects of crude extracts from some edible Thai plants against replication of hepatitis B virus and human liver cancer cells. BMC Complement Altern Med, 12, 246. https://doi.org/10.1186/1472-6882-12-246
  24. Wilailak S (2009). Epidemiologic report of gynecologic cancer in Thailand. J Gynecol Oncol, 20, 81-3. https://doi.org/10.3802/jgo.2009.20.2.81
  25. Xiong J, Liu XH, Bui VB, et al (2014). Phenolic constituents from the leaves of Cratoxylum formosum ssp. pruniflorum. Fitoterapia, 94, 114-9. https://doi.org/10.1016/j.fitote.2014.02.002
  26. Xu JG, Hu QP, Liu Y (2012). Antioxidant and DNA-protective activities of chlorogenic acid Isomers. J Agric Food Chem, 60, 11625-30. https://doi.org/10.1021/jf303771s
  27. Zhao B, Hu M (2013). Gallic acid reduces cell viability, proliferation, invasion and angiogenesis in human cervical cancer cells. Oncol Lett, 6, 1749-55.
  28. zur Hausen H (1999). Papillomaviruses in human cancers. Proc Assoc Am Physicians, 111, 581-7. https://doi.org/10.1046/j.1525-1381.1999.99723.x
  29. Martinho O, Pinto F, Granja S, et al (2013). RKIP inhibition in cervical cancer is associated with higher tumor aggressive behavior and resistance to cisplatin therapy. PLoS One, 8, 59104. https://doi.org/10.1371/journal.pone.0059104

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