DOI QR코드

DOI QR Code

Anti-proliferative Activities of Metallic Nanoparticles in an in Vitro Breast Cancer Model

  • Loutfy, Samah A (Virology and Immunology Unit, Cancer Biology Department, National Cancer Institute) ;
  • Al-Ansary, Nadia A (Photochemistry & Photobiology, LAMPA, National Institute of Laser Enhanced Sciences) ;
  • Abdel-Ghani, Nour T (Chemistry Department, Faculty of Science, Cairo University) ;
  • Hamed, Ahmed R (Phytochemistry Department and Center of Excellence for Advanced Sciences, National Research Centre) ;
  • Mohamed, Mona B (Photochemistry & Photobiology, LAMPA, National Institute of Laser Enhanced Sciences) ;
  • Craik, James D (Biochemistry Department, Faculty of Medicine, Health Sciences Center, Kuwait University) ;
  • Eldin, Taher A. Salah (Director of Nanotechnology Characterization Center, Agricultural Research Center) ;
  • Abdellah, Ahmed M (Photochemistry & Photobiology, LAMPA, National Institute of Laser Enhanced Sciences) ;
  • Hussein, Yassmein (Nanotech) ;
  • Hasanin, MTM (Nanotech) ;
  • Elbehairi, Serag Eldin I (Egyptian Organization for Biological Products and Vaccines)
  • 발행 : 2015.09.02

초록

Aims: To investigate effect of metallic nanoparticles, silver (AgNPs) and gold nanoparticles (AuNPs) as antitumor treatment in vitro against human breast cancer cells (MCF-7) and their associated mechanisms. This could provide new class of engineered nanoparticles with desired physicochemical properties and may present newer approaches for therapeutic modalities to breast cancer in women. Materials and Methods: A human breast cancer cell line (MCF-7) was used as a model of cells. Metallic nanoparticles were characterized using UV-visible spectra and transmission electron microscopy (TEM). Cytotoxic effects of metallic nanoparticles on MCF-7 cells were followed by colorimetric SRB cell viability assays, microscopy, and cellular uptake. Nature of cell death was further investigated by DNA analysis and flow cytometry. Results: Treatment of MCF-7 with different concentrations of 5-10nm diameter of AgNPs inhibited cell viability in a dose-dependent manner, with IC50 value of $6.28{\mu}M$, whereas treatment of MCF-7 with different concentrations of 13-15nm diameter of AuNPs inhibited cell viability in a dose-dependent manner, with IC50 value of $14.48{\mu}M$. Treatment of cells with a IC50 concentration of AgNPs generated progressive accumulation of cells in the S phase of the cell cycle and prevented entry into the M phase. The treatment of cells with IC50 concentrations of AuNPs similarly generated progressive accumulation of cells in sub-G1 and S phase, and inhibited the entrance of cells into the M phase of the cell cycle. DNA fragmentation, as demonstrated by electrophoresis, indicated induction of apoptosis. Conclusions: Our engineered silver nanoparticles effectively inhibit the proliferation of human breast carcinoma cell line MCF-7 in vitro at high concentration ($1000{\mu}M$) through apoptotic mechanisms, and may be a beneficial agent against human carcinoma but further detailed study is still needed.

참고문헌

  1. Austin LA, Kang B, Yen CW, et al (2011). Plasmonic imaging of human oral cancer cell communities during programmed cell death by nuclear-targeting silver nanoparticles. J Am Chem Soc, 133, 17594-97. https://doi.org/10.1021/ja207807t
  2. AshaRani PV, Low Kah Mun G, Hande MP, et al (2009). Cytotoxicity and genotoxicity of silver nanoparticles inhuman cells. ACS Nano, 3, 279-90. https://doi.org/10.1021/nn800596w
  3. Buzea C, Pacheco I, Robbie K (2007). Nanomaterials and nanoparticles: sources and toxicity. Biointerphases, 2, 17-71.
  4. Braydich-Stolle L, Hussain S, Schlager JJ, et al (2005). In vitro cytotoxicity of nanoparticles in mammalian germline stem cells. Toxicol Sci, 88, 412-9. https://doi.org/10.1093/toxsci/kfi256
  5. Chithrani DB, Dunne M, Stewart J, et al (2010). Cellular uptake and transport of gold nanoparticles incorporated in a liposomal carrier. Nanomedicine: Nanotechnology, Biology and Medicine, 6, 161-9. https://doi.org/10.1016/j.nano.2009.04.009
  6. CIFTCİH, TURKM, TAMER et al (2013). Silver nanoparticles: cytotoxic, apoptotic and necrotic effects on MCF-7 cells. Turkish Journal of Biology, 37, 573. https://doi.org/10.3906/biy-1302-21
  7. Daduang J, Palasap A, Daduang S, et al (2015). Gallic acid enhancement of gold nanoparticle anticancer activity in cervical cancer cells. APJCC, 16, 169-74.
  8. Elmore S ( 2007). Apoptosis: a review of programmed cell death. Toxicologic pathology, 35, 495-516. https://doi.org/10.1080/01926230701320337
  9. Foldbjerg R, Dang DA, Autrup H (2011). Cytotoxicity and genotoxicity of silver nanoparticles in the human lung cancer cell line, A549. Arch Toxicol, 85, 743-50. https://doi.org/10.1007/s00204-010-0545-5
  10. Gopinath P, Gogoi SK, Chattopadhyay A, (2008). Implications of silver nanoparticle induced cell apoptosis for in vitro gene therapy. Nanotechnology, 19, 1-10.
  11. Hussain SM, hess KL, Gearhart JM, et al (2005). In vitro toxicity of nanoparticles in BRL 3A rat liver cells. Toxicol In vitro, 19, 975-83. https://doi.org/10.1016/j.tiv.2005.06.034
  12. Hussain SM, Javorina AK, Schrand AM, (2006). The interaction of manganese nanoparticles with PC-12 cells induces dopamine depletion. Toxicol Sci, 92, 456-63. https://doi.org/10.1093/toxsci/kfl020
  13. Handley DA (1989). Methods for synthesis of colloidal gold. In colloidal gold principles, methods, and applications; Vol 1 (ed. M.A. Hayat) Academic Press, San Diego.
  14. Hansen MB, Nielsen SE, Berg K (1989). Re-examination and further development of a precise and rapid dye method for measuring cell growth/cell kill. J Immunol Methods, 119, 203-10. https://doi.org/10.1016/0022-1759(89)90397-9
  15. Houghton P, Fang R, TechatanawatI, (2007). The sulphorhodamine (SRB) assay and other approaches to testing plant extracts and derived compounds for Activities related to reputed anticancer activity. Methods, 42, 377-87. https://doi.org/10.1016/j.ymeth.2007.01.003
  16. Kang SJ, Ryoo IG, Lee YJ, Kwak MK (2012). Role of the Nrf2-heme oxygenase-1 pathway in silver nanoparticle-mediated cytotoxicity. Toxicol Appl Pharmacol, 258, 89-98. https://doi.org/10.1016/j.taap.2011.10.011
  17. Kawata K, Osawa M, Okabe S (2009). In vitro of silver nanoparticles at noncytotoxic doses to HepG2 human hepatoma cells. Environ Sci Technol, 43, 6046-51. https://doi.org/10.1021/es900754q
  18. Nunez R (2001). DNA measurement and cell cycle analysis by flow cytometry. Curr Issues Mol Biol, 3, 67-70.
  19. Priya KMR and lyer PR (2015). Anticancer studies of the synthesized gold nanoparticles against MCF 7 breast cancer cell lines. Applied nanoscience, 5, 443-8. https://doi.org/10.1007/s13204-014-0336-z
  20. Samah A Loutfy, Mona Bakr Mohamed, Nour Tawfik Abdel- Ghani, et al, (2013). Metallic nanomaterials as drug carriers to decrease side effects of chemotherapy (In vitro: Cytotoxicity Study). J Nanopharmaceutics Drug Delivery, 1, 138-49. https://doi.org/10.1166/jnd.2013.1010
  21. Samberg ME, Oldenburg SJ, Monteiro-Riviere NA (2010). Evaluation of silver nanoparticle toxicity in skin in vivo and keratinocytes in vitro. Environ Health Perspect, 118, 407-13.
  22. Schmidt NJ, Emmons RW (1989). Diagnostic procedures for viral, rickettsial, and chlamydial Infections. American Public Health Association Washington, DC 1989, 957-1065.
  23. Selim ME, Hendi AA (2012). Gold nanoparticles induce apoptosis in MCF-7 human breast cancer cells. Asian Pac J of Cancer Prev, 13, 1617-20. https://doi.org/10.7314/APJCP.2012.13.4.1617
  24. Skehan P , Storeng R, Scudiero D, et al (1990). New colorimetric cytotoxicity assay for anti-cancer drug screening. J Natl cancer Inst, 82, 1107-12. https://doi.org/10.1093/jnci/82.13.1107
  25. Sutradhar KB and Amin L (2014). Nanotechnology in cancer drug delivery and selective Targeting. ISRN Nanotechnology, 939378, 1-12.
  26. Su XY, Liu PD, Wu H, et al, (2014). Enhancement of radiosensitization by metal-based nanoparticles in cancer radiation therapy. Cancer Biol Med, 11, 86-91.
  27. Vijayakumar S and Ganesan S (2012). In vitro cytotoxicity assay on gold nanoparticles with different stabilizing agents. J of nanomaterials, 734398, 9.
  28. Wang Y, Jiang JD, Xu D (2004). Mouse mammary tumor viruslike long terminal repeat superantigen in human breast cancer. Cancer Res, 64, 4105-4111. https://doi.org/10.1158/0008-5472.CAN-03-3880
  29. Wen HC, Lin YN, Jian SR, et al (2007). Observation of growth of human fibroblasts on silver nanoparticles. J Phys, 61, 445-9.
  30. Wlodkowic D, Telford W, Skommer J, et al (2011). Apoptosis and Beyond: Cytometry in studies of programmed cell death. Methods in cell biology, 103, 55-98.
  31. Zhu ZJ, Ghosh PS, Miranda OR (2008). Multiplexed screening of cellular uptake of gold nanoparticles using laser desorption/ionization mass spectrometry. J Am Chem Soc, 130, 14139-43. https://doi.org/10.1021/ja805392f

피인용 문헌

  1. In Vitro Study of Influence of Au Nanoparticles on HT29 and SPEV Cell Lines vol.12, pp.1, 2017, https://doi.org/10.1186/s11671-017-2264-9
  2. Antitumor Activity of Alloy and Core-Shell-Type Bimetallic AgAu Nanoparticles vol.12, pp.1, 2017, https://doi.org/10.1186/s11671-017-2112-y
  3. Cyto-toxicity, biocompatibility and cellular response of carbon dots–plasmonic based nano-hybrids for bioimaging vol.7, pp.38, 2017, https://doi.org/10.1039/C7RA01423F
  4. Aqueous Bulb Extract pp.1793-5350, 2018, https://doi.org/10.1142/S0219581X18500230
  5. A Current Overview of the Biological and Cellular Effects of Nanosilver vol.19, pp.7, 2018, https://doi.org/10.3390/ijms19072030