- Volume 16 Issue 14
DOI QR Code
Anti-proliferative Activities of Metallic Nanoparticles in an in Vitro Breast Cancer Model
- Loutfy, Samah A (Virology and Immunology Unit, Cancer Biology Department, National Cancer Institute) ;
- Al-Ansary, Nadia A (Photochemistry & Photobiology, LAMPA, National Institute of Laser Enhanced Sciences) ;
- Abdel-Ghani, Nour T (Chemistry Department, Faculty of Science, Cairo University) ;
- Hamed, Ahmed R (Phytochemistry Department and Center of Excellence for Advanced Sciences, National Research Centre) ;
- Mohamed, Mona B (Photochemistry & Photobiology, LAMPA, National Institute of Laser Enhanced Sciences) ;
- Craik, James D (Biochemistry Department, Faculty of Medicine, Health Sciences Center, Kuwait University) ;
- Eldin, Taher A. Salah (Director of Nanotechnology Characterization Center, Agricultural Research Center) ;
- Abdellah, Ahmed M (Photochemistry & Photobiology, LAMPA, National Institute of Laser Enhanced Sciences) ;
- Hussein, Yassmein (Nanotech) ;
- Hasanin, MTM (Nanotech) ;
- Elbehairi, Serag Eldin I (Egyptian Organization for Biological Products and Vaccines)
- Published : 2015.09.02
Aims: To investigate effect of metallic nanoparticles, silver (AgNPs) and gold nanoparticles (AuNPs) as antitumor treatment in vitro against human breast cancer cells (MCF-7) and their associated mechanisms. This could provide new class of engineered nanoparticles with desired physicochemical properties and may present newer approaches for therapeutic modalities to breast cancer in women. Materials and Methods: A human breast cancer cell line (MCF-7) was used as a model of cells. Metallic nanoparticles were characterized using UV-visible spectra and transmission electron microscopy (TEM). Cytotoxic effects of metallic nanoparticles on MCF-7 cells were followed by colorimetric SRB cell viability assays, microscopy, and cellular uptake. Nature of cell death was further investigated by DNA analysis and flow cytometry. Results: Treatment of MCF-7 with different concentrations of 5-10nm diameter of AgNPs inhibited cell viability in a dose-dependent manner, with IC50 value of
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