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Common Genetic Variants of PSCA, MUC1 and PLCE1 Genes are not Associated with Colorectal Cancer

  • Kupcinskas, Juozas (Department of Gastroenterology, Lithuanian University of Health Sciences) ;
  • Gyvyte, Ugne (Institute for Digestive Research, Lithuanian University of Health Sciences) ;
  • Bruzaite, Indre (Institute for Digestive Research, Lithuanian University of Health Sciences) ;
  • Leja, Marcis (Faculty of Medicine, University of Latvia, Digestive Diseases Center GASTRO, Riga East University Hospital) ;
  • Kupcinskaite-Noreikiene, Rita (Institute for Digestive Research, Lithuanian University of Health Sciences) ;
  • Pauzas, Henrikas (Department of Surgery, Lithuanian University of Health Sciences) ;
  • Tamelis, Algimantas (Department of Surgery, Lithuanian University of Health Sciences) ;
  • Jonaitis, Laimas (Department of Gastroenterology, Lithuanian University of Health Sciences) ;
  • Skieceviciene, Jurgita (Institute for Digestive Research, Lithuanian University of Health Sciences) ;
  • Kiudelis, Gediminas (Department of Gastroenterology, Lithuanian University of Health Sciences)
  • Published : 2015.09.02

Abstract

Background: Polymorphisms of genes encoding PSCA, PLCE1 and MUC1 have been associated with the risk of different cancers in genome wide association studies (GWAS). Up to date there are limited data on the role of these genetic alterations in colorectal cancer (CRC) development. The aim of this study was to evaluate potential associations between single nucleotide polymorphisms (SNPs) of genes encoding PSCA, PLCE1 and MUC1 and the presence of CRC in European populations. Materials and Methods: Gene polymorphisms were analyzed in 574 European subjects (controls: n=382; CRC: n=192). PSCA C>T (rs2294008), PSCA G>A (rs2976392), MUC1 A>G (rs4072037) and PLCE1 A>G (rs2274223) SNPs were genotyped by RT-PCR. Results: The distribution of genotypes for all four SNPs was in line with the Hardy-Weinberg equilibrium (rs2294008, P=0.153; rs2976392, P=0.269; rs4072037, P=0.609; rs2274223, P=0.858). The distribution of genotypes and alleles of PSCA C>T, PSCA G>A, MUC1 A>G and PLCE1 A>G SNPs was similar among controls and CRC patient groups (P>0.05). GG genotype of MUC1 SNP was more frequent in CRC patients (24.0%) than in controls (20.2%); however, this association failed to reach significance (OR-1.45, P=0.15). Overall, in the present study SNPs of PSCA (rs2294008, rs2976392), MUC1 (rs4072037) and PLCE1 (rs2274223) genes were not associated with the presence of CRC. Conclusions: Gene polymorphisms of PSCA, PLCE1 and MUC1 genes are not associated with the presence of CRC in European subjects.

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