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Altered Expression of Oxidative Metabolism Related Genes in Cholangiocarcinomas

  • Aukkanimart, Ratchadawan (Department of Parasitology, Faculty of Medicine, Khon Kaen University) ;
  • Boonmars, Thidarut (Department of Parasitology, Faculty of Medicine, Khon Kaen University) ;
  • Juasook, Amornrat (Liver Fluke and Cholangiocarcinoma Research Center, Faculty of Medicine, Khon Kaen University) ;
  • Sriraj, Pranee (Department of Parasitology, Faculty of Medicine, Khon Kaen University) ;
  • Boonjaraspinyo, Sirintip (Department of Parasitology, Faculty of Medicine, Khon Kaen University) ;
  • Wu, Zhiliang (Department of Parasitology, Graduate School of Medicine, Gifu University) ;
  • Laummuanwai, Porntip (Department of Parasitology, Faculty of Medicine, Khon Kaen University) ;
  • Pairojkul, Chawalit (Department of Pathology, Faculty of Medicine, Khon Kaen University) ;
  • Khuntikeo, Narong (Department of Surgery, Faculty of Medicine, Khon Kaen University) ;
  • Rattanasuwan, Panaratana (Department of Anesthesiology, Faculty of Medicine, Khon Kaen University)
  • Published : 2015.09.02

Abstract

Cholangiocarcinoma (CCA) is a rare but highly fatal cancer for which the molecular mechanisms and diagnostic markers are obscure. We therefore investigated the kinetic expression of isocitrate dehydrogenase-1 (IDH1), isocitrate dehydrogenase-2 (IDH2) and homogentisate 1,2-dioxygenase (HGD) during the tumorigenesis of O. viverrini infection-associated CCA in an animal model, and confirmed down-regulation of expression in human cases of opisthorchiasis-associated CCA through real time PCR. Kinetic expression of HGD, IDH1 and IDH2 in the animal model of O. viverrini infection-induced CCA was correlated with human CCA cases. In the animal model, expression of HGD was decreased at all time points (p<0.01) and expression of both IDH1 and IDH2 was decreased in the CCA group. In human cases, expression of HGD, IDH1 and IDH2 was decreased more than 2 fold in 55 cases (70.5%), 25 cases (32.1%) and 24 cases (30.8%) respectively. The present study suggests that reduction of HGD, IDH1 and IDH2 may be involve in cholangiocarcinoma genesis and may be useful for molecular diagnosis.

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