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A Promising Serum Autoantibody Marker, Anti-Heat Shock Protein 90α, for Cholangiocarcinoma

  • Boonjaraspinyo, Sirintip (Department of Community Medicine, Liver Fluke and Cholangiocarcinoma Research Center) ;
  • Juasook, Amornrat (Faculty of Veterinary Medicine, Mahasarakham University) ;
  • Boonmars, Thidarut (Neglected and Vector Borne-Zoonosis Research Group, Liver Fluke and Cholangiocarcinoma Research Center) ;
  • Aukkanimart, Ratchadawan (Neglected and Vector Borne-Zoonosis Research Group, Liver Fluke and Cholangiocarcinoma Research Center) ;
  • Silsirivanit, Atit (Neglected and Vector Borne-Zoonosis Research Group, Liver Fluke and Cholangiocarcinoma Research Center) ;
  • Loilome, Watcharin (Neglected and Vector Borne-Zoonosis Research Group, Liver Fluke and Cholangiocarcinoma Research Center) ;
  • Sriraj, Pranee (Neglected and Vector Borne-Zoonosis Research Group, Liver Fluke and Cholangiocarcinoma Research Center) ;
  • Wu, Zhiliang (Department of Parasitology, Graduate School of Medicine, Gifu University) ;
  • Ratanasuwan, Panaratana (Department of Anesthesiology, Faculty of Medicine, Khon Kaen University)
  • Published : 2015.09.02

Abstract

The present study was designed to investigate cholangiocarcinoma (CCA) antibodies in hamster serum. Hamster CCA cell lines were processed using sodium dodecyl sulfate-polyacrylamide gel electrophoresis and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. A candidate biomarker was confirmed by immunoprecipitation and western blot, and was further analyzed using ELISA and sera from normal control hamsters, hamsters with opisthorchiasis and hamsters with various stages of CCA, as well as from CCA patients and healthy individuals. One candidate marker was identified as $HSP90{\alpha}$, as indicated by a high level of anti-$HSP90{\alpha}$ in hamster CCA sera. It was found that the levels of anti-$HSP90{\alpha}$ were specifically elevated in the sera of hamsters with CCA compared with other groups and progressively increased with the clinical stage. At the cut-off point of 0.4850 on the receiver operating characteristic curve, anti-$HSP90{\alpha}$ could discriminate CCA from healthy control groups with a sensitivity of 76.2%, specificity of 71.4% and total accuracy 75.5%. In the present study, we have shown that anti-$HSP90{\alpha}$ may be a potential useful serum biomarker to discriminate CCA cases from healthy persons.

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