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Expression of Lamininγ2 in Extrahepatic Cholangiocarcinoma Tissues and its Influence on Tumor Invasion and Metastasis

  • Li, Yong (Institute of Hepatobiliary Surgery, Southwest Hospital, Third Military Medical University) ;
  • Li, Da-Jiang (Institute of Hepatobiliary Surgery, Southwest Hospital, Third Military Medical University) ;
  • Chen, Jian (Institute of Hepatobiliary Surgery, Southwest Hospital, Third Military Medical University) ;
  • Liu, Wei (Institute of Hepatobiliary Surgery, Southwest Hospital, Third Military Medical University) ;
  • Li, Jian-Wei (Institute of Hepatobiliary Surgery, Southwest Hospital, Third Military Medical University) ;
  • Jiang, Peng (Institute of Hepatobiliary Surgery, Southwest Hospital, Third Military Medical University) ;
  • Zhao, Xin (Institute of Hepatobiliary Surgery, Southwest Hospital, Third Military Medical University) ;
  • Guo, Fei (Institute of Hepatobiliary Surgery, Southwest Hospital, Third Military Medical University) ;
  • Li, Xiao-Wu (Institute of Hepatobiliary Surgery, Southwest Hospital, Third Military Medical University) ;
  • Wang, Shu-Guang (Institute of Hepatobiliary Surgery, Southwest Hospital, Third Military Medical University)
  • Published : 2015.03.18

Abstract

Objective: To explore the expression of $laminin{\gamma}2$ in extrahepatic cholangiocarcinoma (EHCC) tissues and its influence on tumor invasion and metastasis. Materials and Methods: Paraffin embedding samples of cancer, para-cancer, lymph node metastatic and hepatic metastatic tissues from 79 patients undergoing EHCC resection were collected. Expression of $laminin{\gamma}2$ was detected by immunohistochemistry and its relationship with clinical pathological characteristics and the prognosis of EHCC patients were analyzed. Results: $Laminin{\gamma}2$ showed negative staining in para-cancer tissues, but demonstrated a 51.9% (41/79) positive expression rate in extracellular matrix (ECM) or cytoplasm of EHCC tissues. In lymph node metastatic and distant metastatic nidi, expression of $laminin{\gamma}2$ was significantly higher than in the primary nidi (${\chi}^2=7.4173$, P=0.0065; ${\chi}^2=4.0077$, P=0.0453). The expression was in obvious association with lymph node metastasis (P<0.01), but had no relevance with age, gender, tumor location, tumor stage, differentiation and distant metastasis in ECM (P>0.05), whereas it was in marked connection with lymph node and distant metastasis (P<0.05 or P<0.01), but had no relationship with age, gender, tumor location, tumor stage and differentiation in cytoplasm (P>0.05). However, the median survival time and median recurrent period of patients with positive expression of $laminin{\gamma}2$ in both cytoplasm and ECM of tumor cells, only in ECM and only in cytoplasm, were evidently lower than with negative expression of $laminin{\gamma}2$ in RCM and cytoplasm (P<0.05 or P<0.01). Further Cox regression analysis showed that the positive expression of $laminin{\gamma}2$ and the tumor differentiation were independent risk factors influencing the prognosis of EHCC patients. Conclusions: Abnormal expression of $laminin{\gamma}2$ may be closely associated with invasion and metastasis of tumor cells, and thus a potential molecular marker for prognosis of EHCC patients.

Keywords

$Laminin{\gamma}2$;extrahepatic cholangiocarcinoma;lymph node metastasis;distant metastasis

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