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Anti-Angiogenic Activity of Gecko Aqueous Extracts and its Macromolecular Components in CAM and HUVE-12 Cells

  • Tang, Zhen (Department of Biological Pharmaceutics, School of Chinese Materia Medica, Beijing University of Chinese Medicine) ;
  • Huang, Shu-Qiong (Department of Biological Pharmaceutics, School of Chinese Materia Medica, Beijing University of Chinese Medicine) ;
  • Liu, Jian-Ting (Department of Biological Pharmaceutics, School of Chinese Materia Medica, Beijing University of Chinese Medicine) ;
  • Jiang, Gui-Xiang (Department of Biological Pharmaceutics, School of Chinese Materia Medica, Beijing University of Chinese Medicine) ;
  • Wang, Chun-Mei (Department of Biological Pharmaceutics, School of Chinese Materia Medica, Beijing University of Chinese Medicine)
  • Published : 2015.03.18

Abstract

Gecko is a kind of traditional Chinese medicine with remarkable antineoplastic activity. However, undefined mechanisms and ambiguity regarding active ingredients limit new drug development from gecko. This study was conducted to assess anti-angiogenic properties of the aqueous extracts of fresh gecko (AG) or macromolecular components separated from AG (M-AG). An enzyme-linked immunosorbent assay (ELISA) approach was applied to detect the vascular endothelial growth factor (VEGF) secretion of the tumor cells treated with AG or M-AG. The effect of AG or M-AG on vascular endothelial cell proliferation and migratory ability was analyzed by tetrazolium dye colorimetric method, transwell and wound-healing assays. Chick embryo chorioallantoic membrane (CAM) assays were used to ensure the anti-angiogenic activity of M-AG in vivo. The results showed that AG or M-AG inhibited the VEGF secretion of tumor cells, the relative inhibition rates of AG and M-AG being 27.2% and 53.2% respectively at a concentration of $20{\mu}L/mL$. AG and M-AG inhibited the vascular endothelial (VE) cell proliferation with IC50 values of $11.5{\pm}0.5{\mu}L/mL$ and $12.9{\pm}0.4{\mu}L/mL$ respectively. The VE cell migration potential was inhibited significantly (p<0.01) by the AG (${\geq}24{\mu}L/mL$) or M-AG (${\geq}12\mu}L/mL$) treatment. In vivo, neovascularization of CAM treated with M-AG was inhibited significantly (p<0.05) at a concentration of ${\geq}0.4{\mu}L/mL$. This study provided evidence that anti-angiogenesis is one of the anti-tumor mechanisms of AG and M-AG, with the latter as a promising active component.

Keywords

Gecko;anti-angiogenesis;aqueous extracts;macromolecular component;vascular endothelial growth

Acknowledgement

Supported by : Beijing University

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