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P53 Polymorphism at Codon 72 is Associated with Keratocystic Odontogenic Tumors in the Thai Population

  • Yanatatsaneejit, Pattamawadee (Human Genetics Research Group, Department of Botany, Faculty of Science) ;
  • Boonsrang, Ajaree (Human Genetics Research Group, Department of Botany, Faculty of Science) ;
  • Mutirangura, Apiwat (Center of Excellence in Molecular Genetics of Cancer and Human Diseases, Department of Anatomy, Faculty of Medicine, Chulalongkorn University) ;
  • Patel, Vyomesh (Cancer Research Initiatives Foundation (CARIF), 2nd Floor Outpatient Centre, Sime Darby Medical Centre) ;
  • Kitkumthorn, Nakarin (Department of Oral and Maxillofacial Pathology, Faculty of Dentistry, Mahidol University)
  • Published : 2015.03.18

Abstract

Objective: To clarify the association between the p53 polymorphism at codon 72 and susceptibility to the sporadic keratocystic odontogenic tumor (KCOT). Design: One hundred KCOTs and 160 match-group healthy controls were genotyped to ascertain the frequency of the p53 codon 72 polymorphism using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP), confirmed by direct sequencing. Results: The frequencies of the Pro/Pro, Arg/Pro, and Arg/Arg genotypes were 23.8%, 49.4%, and 26.9%, respectively, in the controls, while the KCOT cohort demonstrated 43.0%, 39.0%, and 18.0%, respectively. Further analysis suggested that p53 Pro could be a KCOT-susceptible allele (OR (95%CI)=1.77 (1.22 to 2.59), p=0.0024), with a sex-adjusted OR (95%CI) of 1.71 (1.17-2.50), p=0.0046. Moreover, the results indicated that p53 codon 72 Pro homozygous was associated with a two-fold risk of developing KCOT (adjusted OR (95%CI) =2.17(1.23-3.84), p=0.0062). Conclusions: The C/C genotype of P53 gene codon 72 increases the risk of developing sporadic KCOT and may be a useful tool for screening and diagnostic purposes.

Keywords

Keratocystic odontogenic tumor;P53;polymorphism;risk

Acknowledgement

Supported by : Chulalongkorn University

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