DOI QR코드

DOI QR Code

IL-1β Polymorphism and Expression Associated with Decreased Risk of Gastric Carcinoma: a Case Control Study in the Ethnic Kashmiri Population, India

  • Irtiza, Syed (Department of Biochemistry, Faculty of Life Sciences, Jamia Hamdard University) ;
  • Samie, Amat Us (Department of General surgery, Sher-I-Kashmir Institute of Medical Sciences) ;
  • Ali, Shakir (Department of Microbiology, Medical College, Sher-I-Kashmir Institute of Medical Sciences) ;
  • Siddiqi, Mushtaq A (Taum Charitable Trust) ;
  • Naqash, Sameer H (Department of General surgery, Sher-I-Kashmir Institute of Medical Sciences) ;
  • Sameer, Aga Syed (Department of Biochemistry, Sher-I-Kashmir Institute of Medical Sciences Associated Medical College)
  • Published : 2015.03.18

Abstract

The aim of this research was to investigate the possible association between gastric carcinoma (GC) and polymorphisms of the IL-$1{\beta}$ gene in the Kashmiri population using peripheral blood DNA from 150 gastric carcinoma cases and 250 population controls with detailed data for clinicopathological characteristics of the disease. Two SNPs in the IL-$1{\beta}$ gene were selected for this study. Expression of IL-$1{\beta}$ was studied in 50 gastric carcinoma cases using immunohistochemistry and RT-PCR and then correlated with genotype. The frequency of the IL-$1{\beta}$-511 C allele was significantly higher in the GC case group (53.3%) than in controls (45.4%) with an odds ratio (OR) of 0.73 and a P value of 0.03. Multivariate regression analysis showed associations of gastric carcinoma with mutant form of IL-$1{\beta}$-511 TT (OR 0.309; P value <0.001) and the CC genotype of IL-$1{\beta}$-31 (OR 0.313; P value of 0.002). Haplotype analysis of IL-$1{\beta}$-31 and IL-$1{\beta}$-511 showed decreased association of IL-$1{\beta}$-31 T with IL-$1{\beta}$-511 C with gastric carcinoma (OR 0.728; P value 0.03). Expression study of 50 samples by immunohistochemistry (IHC) and RT-PCR showed association with grade III and stage III+IV. After correlating the expression with polymorphism no association was found.

Keywords

Gastric carcinoma;interleukin polymorphisms;IHC;Kashmiri population;India

References

  1. Ahmad ST, Seth A, Nafees S, et al (2011). Preclinical renal cancer chemopreventive efficacy of geraniol by modulation of multiple molecular pathways. Toxicology, 290, 69-81. https://doi.org/10.1016/j.tox.2011.08.020
  2. Bidwell J1, Keen L, Gallagher G, Kimberly R, Huizinga T, et al (1999). Cytokine gene polymorphism in human disease: on-line databases. Genes Immun, 1, 3-19. https://doi.org/10.1038/sj.gene.6363645
  3. Chang YT, Wu MS, Shun CT, Lin MT, Chang MC, Lin JT (2002). Association of polymorphisms of interleukin-1 beta gene and Helicobacter pylori infection with the risk of gastric ulcer. Hepatogastroenterol, 49, 1474-6.
  4. Chang YW, Jang JY, Kim NH, Lee JW, Lee HJ, Jung WW, (2005). Interleukin-1B (IL-1B) polymorphisms and gastric mucosal levels of IL-$1{\beta}$ cytokine in Korean patients with gastric cancer. Int J Cancer, 114, 465-71. https://doi.org/10.1002/ijc.20724
  5. Duan Y, Shi JN, Pan C, Chen HL, Zhang SZ (2014). Association between the interleukin-17A -197G>A (rs2275913) polymorphism and risk of digestive cancer. Asian Pac J Cancer Prev, 15, 9295-300. https://doi.org/10.7314/APJCP.2014.15.21.9295
  6. El-Omar EM, Carrington M, Chow WH, et al (2000). Interleukin-1 polymorphisms associated with increased risk of gastric cancer. Nature, 404, 398-402 https://doi.org/10.1038/35006081
  7. El-Omar EM, Gammon MD, Vaughan TL, et al (2003). Increased risk of non-cardia gastric cancer associated with proinflammatory cytokine gene polymorphisms. Gastroenterol, 124, 1193-201. https://doi.org/10.1016/S0016-5085(03)00157-4
  8. Hamajima N, Ito H, Matsuo K, Tajima K, Tominaga S (2002). Helicobacter pylori seropositivity, the interleukin 1B polymorphism and smoking among first-visit outpatients. Asian Pac J Cancer Prev, 3, 23-28.
  9. Kamangar F, Cheng C, Abnet CC, Rabkin CS (2006). Interleukin- 1B polymorphisms and gastric cancer risk-a meta-analysis. Cancer Epidemiol Biomarkers Prev, 15, 1920-8. https://doi.org/10.1158/1055-9965.EPI-06-0267
  10. Kato S, Onda M, Yamada S, Matsuda N, Tokunaga A, Matsukura N (2001). Association of the interleukin-1 beta genetic polymorphism and gastric cancer risk in Japanese. J Gastroenterol, 36, 696-9. https://doi.org/10.1007/s005350170033
  11. Khuroo MS, Mahajan R, Banday MA (1992). High incidence of oesophageal and gastric cancer in Kashmir in a population with special personal and dietary habits. Gut, 33, 11-15. https://doi.org/10.1136/gut.33.1.11
  12. Kikuchi M, Hishida A, Ishikawa K, et al (2002). Associations between serum C reactive protein (CRP) levels in interleukin 1B, and tumor necrosis factor genes among Japanese health checkup examinees. Asian Pac J Cancer Prev, 3, 23-8.
  13. Machado JC, Canedo P, Pharoah P, et al (2003). A proinflammatory genetic profile increases the risk for chronic atrophic gastritis and gastric carcinoma. Gastroenterology, 125, 364-371. https://doi.org/10.1016/S0016-5085(03)00899-0
  14. Machado JC, Sousa S, Carvalho R, et al (2001). Interleukin 1B and interleukin 1RN polymorphisms are associated with increased risk of gastric carcinoma. Gastroenterology, 121, 823-829. https://doi.org/10.1053/gast.2001.28000
  15. Malaty HM, Kim JG, El-Zimaity HM, Graham DY (1997). High prevalence of duodenal ulcer and gastric cancer in dyspeptic patients in Korea. Scand. J Gastroenterol, 32, 751-4.
  16. Martinez-Carrillo DN, Garza-Gonzalez E, Betancourt-Linares R, et al (2010). Association of IL1B -511C/-31T haplotype and Helicobacter pylori vacA genotypes with gastric ulcer and chronic gastritis. BMC Gastroenterol, 10, 126. https://doi.org/10.1186/1471-230X-10-126
  17. Matsukura N, Yamada S, Kato S, et al (2003). Genetic differences in interleukin-1 beta polymorphisms among four Asian populations: An analysis of the Asian paradox between H. pylori infection and gastric cancer incidence. J Exp Clin Cancer Res, 22, 47-55.
  18. Nomura A, Grove JS, Stemmermann GN, Severson RK (1990). A prospective study of stomach cancer and its relation to diet, cigarettes, and alcohol consumption. Cancer Res, 50, 627-31.
  19. Parkin DM, Ferlay J, Pisani P (2005). Global cancer statistics, 2002. CA Cancer J Clin, 55, 74-108. https://doi.org/10.3322/canjclin.55.2.74
  20. Parkin DM, Muir CS (1988). Estimates of the worldwide frequency of sixteen major cancers in 1980. Int J Cancer, 41, 184-197. https://doi.org/10.1002/ijc.2910410205
  21. Pyo C-W, Kim YK, Choi H-B, et al (2003). Polymorphisms of IL-1B, IL-1RN, IL-2, IL-4, IL-6, IL-10, and IFN- genes in the Korean population. Human Immunology, 64, 979-89. https://doi.org/10.1016/S0198-8859(03)00173-3
  22. Xu H, Ding Q, Jiang HW (2014). Genetic polymorphism of interleukin-1A (IL-1A), IL-1B and IL-1 receptor antagonist (IL-1RN) and prostate cancer risk. Asian Pac J Cancer Prev, 15, 8741-7. https://doi.org/10.7314/APJCP.2014.15.20.8741
  23. Yang J, Hu Z, Xu Y, Shen J, Niu J, Hu X, et al (2004). Interleukin-1B gene promoter variants are associated with an increased risk of gastric cancer in a Chinese population. Cancer Letters, 215, 191-8. https://doi.org/10.1016/j.canlet.2004.07.012
  24. Yang L, Sun MJ, Liu JW, Xu Q, Yuan Y (2013). IL-6-6331 (T/C, rs10499563) is associated with decreased risk of gastric cancer in Northern Chinese. Asian Pac J Cancer Prev, 14, 7467-72. https://doi.org/10.7314/APJCP.2013.14.12.7467
  25. You WC, Blot WJ, Chang YS, et al (1988). Diet and high risk of stomach cancer in Shandong, China. Cancer Res, 48, 3518-23.
  26. Yu H, Sun S, Liu F, Xu QH (2014). Meta-analysis of associations between interleukin-17 gene polymorphisms and risk of gastric cancer. Asian Pac J Cancer Prev, 15, 8709-13. https://doi.org/10.7314/APJCP.2014.15.20.8709
  27. Zeng ZR, Hu PJ, Hu S, Pang RP, Chen MH, Ng M, et al (2003). Association of interleukin 1B gene polymorphism and gastric cancers in high and low prevalence regions in China. Gut, 52, 1684-9. https://doi.org/10.1136/gut.52.12.1684