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Clinical Significance of Upregulation of mir-196a-5p in Gastric Cancer and Enriched KEGG Pathway Analysis of Target Genes

  • Li, Hai-Long (Department of Clinical Testing Teaching and Research, School of Medical Technology, Gansu Traditional Chinese Medical University) ;
  • Xie, Shou-Pin (First People's Hospital of Lanzhou City) ;
  • Yang, Ya-Li (Department of Clinical Testing Teaching and Research, School of Medical Technology, Gansu Traditional Chinese Medical University) ;
  • Cheng, Ying-Xia (Key Laboratory of Traditional Chinese Herbs and Prescription Innovation and Transformation in Gansu Province, Gansu Traditional Chinese Medical University) ;
  • Zhang, Ying (Department of Dermatology, The First Hospital of Chinese People's Liberation Army) ;
  • Wang, Jing (Department of Clinical Testing Teaching and Research, School of Medical Technology, Gansu Traditional Chinese Medical University) ;
  • Wang, Yong (Department of Clinical Testing Teaching and Research, School of Medical Technology, Gansu Traditional Chinese Medical University) ;
  • Liu, Da-Long (First People's Hospital of Lanzhou City) ;
  • Chen, Zhao-Feng (Division of Gastroenterology and Hepatology, First Hospital of Lanzhou University) ;
  • Zhou, Yong-Ning (Division of Gastroenterology and Hepatology, First Hospital of Lanzhou University) ;
  • Wu, Hong-Yan (First People's Hospital of Lanzhou City)
  • Published : 2015.03.18

Abstract

Background: miRNAs are relatively recently discovered cancer biomarkers which have important implications for cancer early diagnosis, treatment and estimation of prognosis. Here we focussed on expression of mir-196a-5p in gastric cancer tissues and cell lines so as to analyse its significance for clinicopathologic characteristics and generate enriched KEGG pathways clustered by target genes for exploring its potential roles as a biomarker in gastric cancer. Materials and Methods: The expression of mir-196a-5p in poorly, moderate and well differentiated gastric cancer cell lines compared with GES-1 was detected by RT-qPCR, and the expression of mir-196a-5p in gastric cancer tissues comparing with adjacent non cancer tissues of 58 cases were also assessed by RT-qPCR. Subsequently, an analysis of clinical significance of mir-196a-5p in gastric cancer and enriched KEGG pathways was executed based on the miRWalk prediction database combined with bioinformatics tools DAVID 6.7 and Mirfocus 3.0. Results: RT-qPCR showed that mir-196a-5p was up-regulated in 6 poorly and moderate differentiated gastric cancer cell lines SGC-7901, MKN-45, MKN-28, MGC-803, BGC-823, HGC-27 compared with GES-1, but down-regulated in the highly differentiated gastric cancer cell line AGS. Clinical data indicated mir-196a-5p to beup-regulated in gastric cancer tissues (47/58). Overexpression of mir-196a-5p was associated with more extensive degree of lymph node metastasis and clinical stage (P < 0.05; x2 test). Enriched KEGG pathway analyses of predicted and validated targets in miRWalk combined with DAVID 6.7 and Mirfocus 3.0 showed that the targeted genes regulated by mir-196a-5p were involved in malignancy associated biology. Conclusions: Overexpression of mir-196a-5p is associated with lymph node metastasis and clinical stage, and enriched KEGG pathway analyses showed that targeted genes regulated by mir-196a-5p may contribute to tumorgenesis, suggesting roles as an oncogenic miRNA biomarker in gastric cancer.

Keywords

Gastric cancer;mir-196a-5p;oncogenic gene;KEGG pathway

Acknowledgement

Supported by : Foundation of the fundamental scientific research funds

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