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Lack of Influence of TP53 Arg72Pro and 16bp Duplication Polymorphisms on Risk of Breast Cancer in Iran

  • Gohari-Lasaki, Sahar (Department of Medical Genetics, School of Medicine, Hematology and Oncology Research Center, Tabriz University of Medical Sciences) ;
  • Gharesouran, Jalal (Department of Medical Genetics, School of Medicine, Hematology and Oncology Research Center, Tabriz University of Medical Sciences) ;
  • Ghojazadeh, Morteza (Department of RDCC, School of Medicine, Hematology and Oncology Research Center, Tabriz University of Medical Sciences) ;
  • Montazeri, Vahid (Department of General Surgery, School of Medicine, Hematology and Oncology Research Center, Tabriz University of Medical Sciences) ;
  • Ardebili, Seiied Moitaba Mohaddes (Department of Medical Genetics, School of Medicine, Hematology and Oncology Research Center, Tabriz University of Medical Sciences)
  • Published : 2015.04.14

Abstract

TP53 is assumed to be a very important tumour suppressor gene, as illustrated by recent reports that have shown effects of its polymorphisms on breast cancer risk. Arg72Pro and PIN3(16bp duplication) polymorphisms are proposed to have an effective role in structural changes of p53 and have therefore attracted interest as a risk factor for breast cancer in different populations. The aim of this study was to examine and determine whether p53 codon 72 and PIN3 Ins16 bp may be associated with an increased risk for breast cancer in female patients from the northwest of Iran. Genotyping was performed by PCR-RFLP (polymerase chain reaction-restriction fragment length polymorphism) method for a total of 100 women with breast cancer and 100 healthy women without any background of cancer, focusing on the TP53 Arg72Pro-16Del/Ins haplotypes and the combined genotypes. The results in this study established no statistical significant distinctions between the genotypes and a llele frequency were found for Arg72Pro and PIN3 Ins 16 bp polymorphisms between patients and controls.

Keywords

Breast cancer;polymorphism;TP53;codon 72;PIN3 Ins16bp;Iran

Acknowledgement

Supported by : Tabriz university of medical science

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  2. “p53 Polymorphism at Codon 72 and Breast Cancer” - Letter vol.22, pp.1, 2017, https://doi.org/10.15430/JCP.2017.22.1.55
  3. No association of TP53 codon 72 and intron 3 16-bp duplication polymorphisms with breast cancer risk in Chinese Han women: new evidence from a population-based case–control investigation vol.23, pp.1, 2018, https://doi.org/10.1186/s40001-018-0345-6