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Preventive and Therapeutic Effects of Quercetin on Experimental Radiation Induced Lung Injury in Mice

  • Wang, Juan (Key Laboratory of Cancer Prevention and Therapy, Tianjin Medical University Cancer Institute and Hospital) ;
  • Zhang, Yuan-Yuan (Department of Radiation Oncology, Shandong Cancer Hospital, School of Medicine and Life Sciences, University of Jinan-Shandong Academy of Medical Scientists) ;
  • Cheng, Jian (the Second Hospital of Shandong University) ;
  • Zhang, Jing-Ling (Linyi People's Hospital, Affiliated Hospital of Shandong University) ;
  • Li, Bao-Sheng (Key Laboratory of Cancer Prevention and Therapy, Tianjin Medical University Cancer Institute and Hospital)
  • Published : 2015.04.14

Abstract

Objective: To investigate the protective effect of quercetin on radiation induced lung injury (RILI) and related mechanisms. Materials and Methods: Mice treated with radiation and/or quercetin were sacrificed at 1-8 weeks after irradiation under anesthesia. Lung tissues were collected for histological examination. Immunohistochemistry (IHC) and Western blotting were performed to detect the protein expression of nuclear factor-${\kappa}B$ ($NF-{\kappa}B$) and Mitogen-activated protein kinases (MAPK) pathway. Results: Hematoxylin and eosin (HE) staining showed that radiation controls displayed more severe lung damage than quercetin groups, either high or low dose. Results of IHC and Western blotting demonstrated the expression level of $NF-{\kappa}B$ to be decreased and that of an inhibitor of $NF-{\kappa}B$ ($I{\kappa}b-{\alpha}$) to be increased by the quercetin intervention compared with the radiation control group. Numbers of JNK/SAPK, p38 and p44/p42 positive inflammatory cells were decreased in the radiation+quercetin injection group (P<0.05). Conclusions: Quercetin may play a radio-protective role in mice lung via suppression of $NF-{\kappa}B$ and MAPK pathways.

Keywords

Quercetin;RILI;$NF-{\kappa}B$;MAPK

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