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SIRT7 Exhibits Oncogenic Potential in Human Ovarian Cancer Cells

  • Wang, Hong-Ling (Department of Clinical Laboratory, Fudan University Shanghai Cancer Center) ;
  • Lu, Ren-Quan (Department of Clinical Laboratory, Fudan University Shanghai Cancer Center) ;
  • Xie, Su-Hong (Department of Clinical Laboratory, Fudan University Shanghai Cancer Center) ;
  • Zheng, Hui (Department of Clinical Laboratory, Fudan University Shanghai Cancer Center) ;
  • Wen, Xue-Mei (Department of Clinical Laboratory, Fudan University Shanghai Cancer Center) ;
  • Gao, Xiang (Department of Clinical Laboratory, Fudan University Shanghai Cancer Center) ;
  • Guo, Lin (Department of Clinical Laboratory, Fudan University Shanghai Cancer Center)
  • Published : 2015.04.29

Abstract

Background: Sirtuin7 (SIRT7) is a type of nicotinamide adenine dinucleotide oxidized form (NAD+)-dependent deacetylase and the least understood member of the sirtuins family; it is implicated in various processes, such as aging, DNA damage repair and cell signaling transduction. There is some evidence that SIRT7 may function as a tumor trigger for human malignancy. Here, we aimed to explore the biological function of SIRT7 in ovarian carcinoma cells and its potential mechanism. Materials and Methods: Expression of SIRT7 in ovarian cancer cell lines was detected by western blotting. Transduced cell lines with SIRT7 knockdown or overexpression were constructed. Cell viability, cologenic, apoptosis-associated and motility assays were performed to elucidate the biological function of SIRT7 in ovarian cancer cells. Results: SIRT7 demonstrated a higher level in ovarian cancer cell lines compared with normal cells. On the one hand, down-regulation of SIRT7 significantly reduced ovarian cancer cell growth, repressed colony formation and increased cancer cell apoptosis; on the other hand, up-regulation promoted the migration of cancer cells. Additionally, repression of SIRT7 also induced change in apoptosis-related molecules and subunits of the NF-${\kappa}B$ family. Conclusions: In the present study, our data indicated that SIRT7 might play a role of oncogene in ovarian malignancy and be a potential therapeutic target.

Keywords

SIRT7;ovarian cancer cells;deacetylase;NF-${\kappa}B$

Acknowledgement

Supported by : Science and Technology Commission of Shanghai Municipality

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