- Volume 16 Issue 8
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Portulaca oleracea Seed Oil Exerts Cytotoxic Effects on Human Liver Cancer (HepG2) and Human Lung Cancer (A-549) Cell Lines
- Al-Sheddi, Ebtesam Saad (Department of Pharmacognosy, College of Pharmacy, King Saud University) ;
- Farshori, Nida Nayyar (Department of Pharmacognosy, College of Pharmacy, King Saud University) ;
- Al-Oqail, Mai Mohammad (Department of Pharmacognosy, College of Pharmacy, King Saud University) ;
- Musarrat, Javed (Department of Zoology, College of Science, King Saud University) ;
- Al-Khedhairy, Abdulaziz Ali (Department of Zoology, College of Science, King Saud University) ;
- Siddiqui, Maqsood Ahmed (Department of Zoology, College of Science, King Saud University)
- 발행 : 2015.04.29
Portulaca oleracea (Family: Portulacaceae), is well known for its anti-inflammatory, antioxidative, anti-bacterial, and anti-tumor activities. However, cytotoxic effects of seed oil of Portulaca oleracea against human liver cancer (HepG2) and human lung cancer (A-549) cell lines have not been studied previously. Therefore, the present study was designed to investigate the cytotoxic effects of Portulaca oleracea seed oil on HepG2 and A-549 cell lines. Both cell lines were exposed to various concentrations of Portulaca oleracea seed oil for 24h. After the exposure, percentage cell viability was studied by (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) (MTT), neutral red uptake (NRU) assays, and cellular morphology by phase contrast inverted microscopy. The results showed a concentration-dependent significant reduction in the percentage cell viability and an alteration in the cellular morphology of HepG2 and A-549 cells. The percentage cell viability was recorded as 73%, 63%, and 54% by MTT assay and 76%, 61%, and 50% by NRU assay at 250, 500, and
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