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Aberrant Expression of Pim-3 Promotes Proliferation and Migration of Ovarian Cancer Cells

  • Zhuang, Hao (Department of Medical Microbiology, School of Basic Medical Sciences, Department of Obstetrics &Gynecology, Tianjin Medical University) ;
  • Zhao, Man-Yin (Department of Obstetrics &Gynecology, Yantai City Zhifu district Maternal and Child health Hospital) ;
  • Hei, Kai-Wen (Department of Medical Microbiology, School of Basic Medical Sciences, Department of Obstetrics &Gynecology, Tianjin Medical University) ;
  • Yang, Bai-Cai (Department of Medical Microbiology, School of Basic Medical Sciences, Department of Obstetrics &Gynecology, Tianjin Medical University) ;
  • Sun, Li (Second Affiliated Hospital, Tianjin Medical University) ;
  • Du, Xue (General Hospital, Tianjin Medical University) ;
  • Li, Yong-Mei (Department of Medical Microbiology, School of Basic Medical Sciences, Department of Obstetrics &Gynecology, Tianjin Medical University)
  • Published : 2015.04.29

Abstract

Pim kinase-3(Pim-3), a member of serine/threonine protein kinases, has been implicated in multiple human cancers and involved in Myc-induced tumorigenesis. However, little is known regarding its expression and biological function in human ovarian cancer. In this study we showed that the clinical significance and biological functions of Pim-3 in ovarian cancer and found that higher Pim-3 mRNA level are detected in ovarian cancer tissues than those in normal ovarian tissues. There are significant correlations between higher Pim-3 expression levels with the FIGO stage, histopathological subtypes, and distant metastasis in ovarian cancer patients. Lentivirus-mediated gene overexpression of Pim-3 significantly promotes the proliferation and migration of SKOV3 cell lines. Furthermore, MACC1 and Pim-3 expression were significantly correlated in human ovarian cancer cells, and overexpression of Pim-3 in ovary cancer cells increased MACC1 mRNA and protein expression. The data indicate that Pim-3 acts as a putative oncogene in ovary cancer and could be a viable diagnostic and therapeutic target for ovarian cancer.

Keywords

Pim-3;MACC1;expression;tumorigenesis;ovarian cancer

Acknowledgement

Supported by : National Natural Science Foundation of China

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