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Detection of BCR/ABL Fusion Gene by Hematological and Cytogenetical Analysis in Chronic Myeloid Leukemia Patients in Quetta, Pakistan

  • Tahira, Bibi (Department of Biotechnology, Balochistan University of Information Technology, Engineering and Management Sciences) ;
  • Asif, Muhammad (Department of Biotechnology, Balochistan University of Information Technology, Engineering and Management Sciences) ;
  • Khan, Samiullah (Department of Biotechnology, Balochistan University of Information Technology, Engineering and Management Sciences) ;
  • Hussain, Abrar (Department of Biotechnology, Balochistan University of Information Technology, Engineering and Management Sciences) ;
  • Shahwani, Muhammad Naeem (Department of Biotechnology, Balochistan University of Information Technology, Engineering and Management Sciences) ;
  • Malik, Arif (Institute of Molecular Biology and Biotechnology, The University of Lahore) ;
  • Inayatullah, Syed (Department of Biotechnology, Balochistan University of Information Technology, Engineering and Management Sciences) ;
  • Iqbal, Zafar (College of Applied Medical Sciences, King Saud Bin Abdulaziz University for Health Sciences, National Guards Health Affairs) ;
  • Rasool, Mahmood (Center of Excellence in Genomic Medicine Research (CEGMR), King Abdulaziz University)
  • Published : 2015.05.18

Abstract

Background: Chronic myeloid leukemia (CML) is a myeloproliferative disorder of pluripotent stem cells, caused by reciprocal translocation between the long arms of chromosomes 9 and 22, t(9;22)(q34;q11), known as the Philadelphia chromosome. Materials and Methods: A total of 51 CML patients were recruited in this study. Complete blood counts of all CML patients were performed to find out their total leukocytes, hemoglobin and platelets. FISH was performed for the detection of BCR-ABL fusion and cryptogenic tests using bone marrow samples were performed for the conformation of Ph (9;22)(q34;q11) and variant translocation mechanisms. Results: In cytogenetic analysis we observed that out of 51 CML patients 40 (88.9%) were Ph positive and 4 (8.88%) had Ph negative chromosomes. Mean values of WBC 134.5 $10^3/{\mu}l$, hemoglobin 10.44 mg/dl, and platelets 288.6 $10^3/{\mu}l$ were observed in this study. Conclusions: In this study, Ph positive translocation between chromosome (9:22)(q34;q11) were observed in 40 (88.9%) CML patients.

Keywords

Chronic myeloid leukemia;ph chromosome;variant translocation;matinib mesylate

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