Asian Pacific Journal of Cancer Prevention

Asian Pacific Organization for Cancer Prevention (아시아태평양암예방학회)
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Effect of Sesamin on Apoptosis and Cell Cycle Arrest in Human Breast Cancer MCF-7 Cells

  • Siao, An-Ci (Department of Life Sciences, National Central University) ;
  • Hou, Chien-Wei (Department of Biotechnology and Pharmaceutical Technology, Yuanpei University) ;
  • Kao, Yung-Hsi (Department of Life Sciences, National Central University) ;
  • Jeng, Kee-Ching (Department of Pharmacy, Tungs' Taichung MetroHarbor Hospital)
  • Published : 2015.05.18

Abstract

Dietary prevention has been known to reduce breast cancer risk. Sesamin is one of the major components in sesame seeds and has been widely studied and proven to have anti-proliferation and anti-angiogenic effects on cancer cells. In this study, the influence of sesamin was tested in the human breast cancer MCF-7 cell line for cell viability (MTT assay) and cell cycling (flow cytometry). Results showed that sesamin dose-dependently (1, 10 and $50{\mu}M$) reduced the cell viability and increased LDH release and apoptosis (TUNEL assay). In addition, there was a significant increase of sub-G1 phase arrest in the cell cycle after sesamin treatment. Furthermore, sesamin increased the expression of apoptotic markers of Bax, caspase-3, and cell cycle control proteins, p53 and checkpoint kinase 2. Taken together, these results suggested that sesamin might be used as a dietary supplement f or prevention of breast cancer by modulating apoptotic signal pathways and inhibiting tumor cell growth.

Keywords

Sesamin;breast cancer;apoptosis;cell cycle

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References

  1. Caldon CE, Sutherland RL, Musgrove E (2010). Cell cycle proteins in epithelial cell differentiation: implications for breast cancer. Cell Cycle, 9, 1918-28. https://doi.org/10.4161/cc.9.10.11474
  2. Chen HS, Bai MH, Zhang T, Li GD, Liu M (2015). Ellagic acid induces cell cycle arrest and apoptosis through TGF-${\beta}$/Smad3 signaling pathway in human breast cancer MCF-7 cells. Int J Oncol, 46, 1730-8.
  3. Chiang HM, Chang H, Yao PW, et al (2014). Sesamin reduces acute hepatic injury induced by lead coupled with lipopolysaccharide. J Chin Med Assoc, 77, 170-8.
  4. Ciccia A, Elledge SJ (2010). The DNA damage response: making it safe to play with knives. Mol Cell, 40, 179-204. https://doi.org/10.1016/j.molcel.2010.09.019
  5. Esmaeili-Mahani S, Falahi F, Yaghoobi MM (2014). Proapoptotic and antiprolifera- tive effects of Thymus caramanicus on human breast cancer cell line (MCF-7) and its interaction with anticancer drug vincristine. Evid Based Complement Alternat Med, 2014, 893247.
  6. Gullett NP, Ruhul Amin AR, Bayraktar S, et al (2010). Cancer prevention with natural compounds. Semin Oncol, 37, 258-81. https://doi.org/10.1053/j.seminoncol.2010.06.014
  7. Hsieh PF, CW Hou, PW Yao, et al (2011). Sesamin ameliorates oxidative stress and mortality in kainic acid-induced status epilepticus by inhibition of MAPK and COX-2 activation. J Neuroinflamm, 8, 57. https://doi.org/10.1186/1742-2094-8-57
  8. Jemal A, Bray F, Center MM, Ferlay J, Ward E, Forman D (2011). Global cancer statistics. CA Cancer J Clin, 61, 69-90. https://doi.org/10.3322/caac.20107
  9. Kim MY, Park SJ, Shim JW, et al (2015). Naphthazarin enhances ionizing radiation-induced cell cycle arrest and apoptosis in human breast cancer cells. Int J Oncol, 46, 1659-66.
  10. Kong Y, Chen J, Zhou Z, et al (2014). Cucurbitacin E induces cell cycle G2/M phase arrest and apoptosis in triple negative breast cancer. PLoS One, 9, 103760. https://doi.org/10.1371/journal.pone.0103760
  11. Noguchi T, Ikeda K, Sasaki Y, et al (2001). Effects of vitamin E and sesamin on hypertension and cerebral thrombogenesis in stroke-prone spontaneously hypertensive rats. Hypertens Res, 24, 735-42. https://doi.org/10.1291/hypres.24.735
  12. Ouchi M, Ouchi T (2014). Distinct DNA damage determines differential phosphorylation of Chk2. Cancer Biol Ther, 15, 1700-4. https://doi.org/10.4161/15384047.2014.972823
  13. Lee JO, Moon JW, Lee SK, et al (2014). Rhus verniciflua extract modulates survival of MCF-7 breast cancer cells through the modulation of AMPK-pathway. Biol Pharm Bull, 37, 794-801. https://doi.org/10.1248/bpb.b13-00893
  14. Tengku Din TA, Seeni A, Khairi WN, Shamsuddin S, Jaafar H (2014). Effects of rapamycin on cell apoptosis in MCF-7 human breast cancer cells. Asian Pac J Cancer Prev, 15, 10659-63.
  15. Truan JS, Chen JM, Thompson LU (2012). Comparative effects of sesame seed lignan and flaxseed lignan in reducing the growth of human breast tumors (MCF-7) at high levels of circulating estrogen in athymic mice. Nutr Cancer, 64, 65-71. https://doi.org/10.1080/01635581.2012.630165
  16. Vegran F, Boidot R, Solary E, Lizard-Nacol S (2011). A short caspase-3 isoform inhibits chemotherapy-induced apoptosis by blocking apoptosome assembly. PLoS One, 6, 29058. https://doi.org/10.1371/journal.pone.0029058
  17. Wang HM, Cheng KC, Lin CJ, et al (2010). Obtusilactone A and (-)-sesamin induce apoptosis in human lung cancer cells by inhibiting mitochondrial Lon protease and activating DNA damage checkpoints. Cancer Sci, 101, 2612-20. https://doi.org/10.1111/j.1349-7006.2010.01701.x
  18. Yadav V, Sultana S, Yadav J, Saini N (2012). Gatifloxacin Induces S and G2-phase cell cycle arrest in pancreatic cancer cells via p21/p27/p53. PLoS One, 7, 47796. https://doi.org/10.1371/journal.pone.0047796
  19. Yokota T, Matsuzaki Y, Koyama M, et al (2007). Sesamin, a lignan of sesame, down-regulates cyclin D1 protein expression in human tumor cells. Cancer Sci, 98, 1447-53. https://doi.org/10.1111/j.1349-7006.2007.00560.x

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