Effect of Sesamin on Apoptosis and Cell Cycle Arrest in Human Breast Cancer MCF-7 Cells

  • Siao, An-Ci (Department of Life Sciences, National Central University) ;
  • Hou, Chien-Wei (Department of Biotechnology and Pharmaceutical Technology, Yuanpei University) ;
  • Kao, Yung-Hsi (Department of Life Sciences, National Central University) ;
  • Jeng, Kee-Ching (Department of Pharmacy, Tungs' Taichung MetroHarbor Hospital)
  • Published : 2015.05.18


Dietary prevention has been known to reduce breast cancer risk. Sesamin is one of the major components in sesame seeds and has been widely studied and proven to have anti-proliferation and anti-angiogenic effects on cancer cells. In this study, the influence of sesamin was tested in the human breast cancer MCF-7 cell line for cell viability (MTT assay) and cell cycling (flow cytometry). Results showed that sesamin dose-dependently (1, 10 and $50{\mu}M$) reduced the cell viability and increased LDH release and apoptosis (TUNEL assay). In addition, there was a significant increase of sub-G1 phase arrest in the cell cycle after sesamin treatment. Furthermore, sesamin increased the expression of apoptotic markers of Bax, caspase-3, and cell cycle control proteins, p53 and checkpoint kinase 2. Taken together, these results suggested that sesamin might be used as a dietary supplement f or prevention of breast cancer by modulating apoptotic signal pathways and inhibiting tumor cell growth.


Sesamin;breast cancer;apoptosis;cell cycle


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