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Preparation and Evaluation of Chrysin Encapsulated in PLGA-PEG Nanoparticles in the T47-D Breast Cancer Cell Line

  • Mohammadinejad, Sina (Department of Medical Biotechnology, Faculty of Advanced Medical Sciences) ;
  • Akbarzadeh, Abolfazl (Department of Medical Biotechnology, Faculty of Advanced Medical Sciences) ;
  • Rahmati-Yamchi, Mohammad (Department of Medical Biotechnology, Faculty of Advanced Medical Sciences) ;
  • Hatam, Saeid (Department of Biomedical Research Center of Sheffield Hallam University (BMRC)) ;
  • Kachalaki, Saeed (Department of Immunology, Tabriz University of Medical Sciences) ;
  • Zohreh, Sanaat (Hematology and Oncology Research Center, Tabriz University of Medical Sciences) ;
  • Zarghami, Nosratollah (Hematology and Oncology Research Center, Tabriz University of Medical Sciences)
  • Published : 2015.05.18

Abstract

Background: Polymeric nanoparticles are attractive materials that have been widely used in medicine for drug delivery, with therapeutic applications. In our study, polymeric nanoparticles and the anticancer drug, chrysin, were encapsulated into poly (D, L-lactic-co-glycolic acid) poly (ethylene glycol) (PLGA-PEG) nanoparticles for local treatment. Materials and Methods: PLGA: PEG triblock copolymers were synthesized by ring-opening polymerization of D, L-lactide and glycolide as an initiator. The bulk properties of these copolymers were characterized using 1H nuclear magnetic resonance spectroscopy and Fourier transform infrared spectroscopy. In addition, the resulting particles were characterized by scanning electron microscopy. Results: The chrysin encapsulation efficiency achieved for polymeric nanoparticles was 70% control of release kinetics. The cytotoxicity of different concentration of pure chrysin and chrysin loaded in PLGA-PEG ($5-640{\mu}M$) on T47-D breast cancer cell line was analyzed by MTT-assay. Conclusions: There is potential for use of these nanoparticles for biomedical applications. Future work should include in vivo investigation of the targeting capability and effectiveness of these nanoparticles in the treatment of breast cancer.

Keywords

Triblock copolymer;chrysin;encapsulation;drug encapsulation efficiency

References

  1. Alimohammadi YH, Joo SW (2014). PLGA-based nanoparticles as cancer drug delivery systems. Asian Pac J Cancer Prev, 15, 517-35. https://doi.org/10.7314/APJCP.2014.15.2.517
  2. Bravo L (1998). Polyphenols: chemistry, dietary sources, metabolism, and nutritional significance. Nut Rev, 56, 317-33.
  3. Butler MS (2004). The role of natural product chemistry in drug discovery. J Nat Prod, 67, 2141-53. https://doi.org/10.1021/np040106y
  4. Cai K, Dynlacht BD (1998). Activity and nature of p21WAF1 complexes during the cell cycle. Proc Nat Acad Sci USA, 95, 12254-9. https://doi.org/10.1073/pnas.95.21.12254
  5. Cao XZ, Xiang HL, Quan MF, et al (2014). Inhibition of cell growth by BrMC through inactivation of Akt in HER-2/neu-overexpressing breast cancer cells. Oncol Letters, 7, 1632.
  6. Chang H, Mi M, Ling W, et al (2008). Structurally related cytotoxic effects of flavonoids on human cancer cells in vitro. Arch Pharm Res, 31, 1137-44. https://doi.org/10.1007/s12272-001-1280-8
  7. Chin YW, Balunas MJ, Chai HB, et al (2006). Drug discovery from natural sources. AAPS J, 8, 239-53. https://doi.org/10.1007/BF02854894
  8. Cook N, Samman S (1996). Flavonoids-chemistry, metabolism, cardioprotective effects, and dietary sources. J Nutr B, 7, 66-76. https://doi.org/10.1016/0955-2863(95)00168-9
  9. de Bono JS, Tolcher AW, Rowinsky EK (2003). The future of cytotoxic therapy: selective cytotoxicity based on biology is the key. Breast Cancer Res, 5, 154-9. https://doi.org/10.1186/bcr597
  10. DeSantis C, Siegel R, Bandi P, et al (2011). Breast cancer statistics, 2011. CA: Cancer J Clin, 61, 408-18. https://doi.org/10.3322/caac.20134
  11. Dickson M, Schwartz G (2009). Development of cell-cycle inhibitors for cancer therapy. Current Oncol, 16, 36.
  12. Dwivedi M, Sharma S, Shukla P, et al (2014). Development and evaluation of anticancer polymeric nano-formulations containing curcumin and natural bioenhancers. J Biomaterials Tissue Eng, 4, 198-202. https://doi.org/10.1166/jbt.2014.1164
  13. Ferlay J, Shin HR, Bray F, et al (2010). Estimates of worldwide burden of cancer in 2008: GLOBOCAN 2008. Int J Cancer, 127, 2893-917. https://doi.org/10.1002/ijc.25516
  14. Fu M, Wang C, Li Z, et al (2004). Minireview: Cyclin D1: normal and abnormal functions. Endocrinol, 145, 5439-47. https://doi.org/10.1210/en.2004-0959
  15. Gillett C, Fantl V, Smith R, et al (1994). Amplification and overexpression of cyclin D1 in breast cancer detected by immunohistochemical staining. Cancer Res, 54, 1812-7.
  16. Ghasemal S, Nejati-Koshki K, Akbarzadeh A, et al (2013). Inhibitory effects of ${\ss}$-cyclodextrin-helenalin complexes on H-TERT gene expression in the T47D breast cancer cell line - Results of real time quantitative. Asian Pac J Cancer Prev, 14, 6949-53. https://doi.org/10.7314/APJCP.2013.14.11.6949
  17. Ishii Y, Pirkmaier A, Alvarez JV, et al (2006). Cyclin D1 overexpression and response to bortezomib treatment in a breast cancer model. J Nati Cancer Inst, 98, 1238-47. https://doi.org/10.1093/jnci/djj334
  18. Kadir EA, Sulaiman SA, Yahya NK, et al (2013). Inhibitory effects of Tualang honey on experimental breast cancer in rats: a preliminary study. Asian Pac J Cancer Prev, 14, 2249-54. https://doi.org/10.7314/APJCP.2013.14.4.2249
  19. Khacha-Ananda S, Tragoolpua K, Chantawannakul P, et al (2013). Antioxidant and Anti-cancer Cell Proliferation Activity of Propolis Extracts from Two Extraction Methods. Asian Pac J Cancer Prev, 14, 6991-5. https://doi.org/10.7314/APJCP.2013.14.11.6991
  20. Khalil NM, Nascimento TCFd, Casa DM, et al (2013). Pharmacokinetics of curcumin-loaded PLGA and PLGA-PEG blend nanoparticles after oral administration in rats. Colloids Surfaces B Biointerfaces, 101, 353-60. https://doi.org/10.1016/j.colsurfb.2012.06.024
  21. Khoo BY, Chua SL, Balaram P (2010). Apoptotic effects of chrysin in human cancer cell lines. Int J Mol Sci, 11, 2188-99. https://doi.org/10.3390/ijms11052188
  22. Koehn FE, Carter GT (2005). The evolving role of natural products in drug discovery. Nature Rev Drug Disc, 4, 206-20. https://doi.org/10.1038/nrd1657
  23. Malumbres M, Barbacid M (2009). Cell cycle, CDKs and cancer: a changing paradigm. Nat Rev Cancer, 9, 153-66. https://doi.org/10.1038/nrc2602
  24. Monasterio A, Urdaci MC, Pinchuk IV, et al (2004). Flavonoids induce apoptosis in human leukemia U937 cells through caspase-and caspase-calpain-dependent pathways. Nut Cancer, 50, 90-100. https://doi.org/10.1207/s15327914nc5001_12
  25. Network CGA (2012). Comprehensive molecular portraits of human breast tumours. Nature, 490, 61-70. https://doi.org/10.1038/nature11412
  26. Pal-Bhadra M, Ramaiah MJ, Reddy TL, et al (2012). Plant HDAC inhibitor chrysin arrest cell growth and induce p21WAF1 by altering chromatin of STAT response element in A375 cells. BMC Cancer, 12, 180. https://doi.org/10.1186/1471-2407-12-180
  27. Sak K (2014). Characteristic features of cytotoxic activity of flavonoids on human cervical cancer cells. Asian Pac J Cancer Prev, 15, 8007-19. https://doi.org/10.7314/APJCP.2014.15.19.8007
  28. Samarghandian S, Afshari JT, Davoodi S (2011). Chrysin reduces proliferation and induces apoptosis in the human prostate cancer cell line pc-3. Clin, 66, 1073-9. https://doi.org/10.1590/S1807-59322011000600026
  29. Schwartz GK, Shah MA (2005). Targeting the cell cycle: a new approach to cancer therapy. J Clin Oncol, 23, 9408-21. https://doi.org/10.1200/JCO.2005.01.5594
  30. Sherr CJ, Roberts JM (1999). CDK inhibitors: positive and negative regulators of G1-phase progression. Genes Development, 13, 1501-12. https://doi.org/10.1101/gad.13.12.1501
  31. Siegel R, Naishadham D, Jemal A (2012). Cancer statistics, 2012. Ca: Cancer J Clin, 62, 10-29. https://doi.org/10.3322/caac.20138
  32. Suganya J, Radha M, Naorem DL, et al (2014). In silico docking studies of selected flavonoids-natural healing agents against breast cancer. Asian Pac J Cancer Prev, 15, 8155-9. https://doi.org/10.7314/APJCP.2014.15.19.8155
  33. Sui JQ, Xie KP, Zou W, et al (2014). Emodin inhibits breast cancer cell proliferation through the ERalpha-MAPK/Akt-cyclin D1/Bcl-2 signaling pathway. Asian Pac J Cancer Prev, 15, 6247-51. https://doi.org/10.7314/APJCP.2014.15.15.6247
  34. Walle T, Otake Y, Brubaker J, et al (2001). Disposition and metabolism of the flavonoid chrysin in normal volunteers. British J Clin Pharm, 51, 143-6. https://doi.org/10.1111/j.1365-2125.2001.01317.x
  35. Yin H-T, Zhang D, Wu X, et al (2013). In vivo evaluation of curcumin-loaded nanoparticles in a A549 xenograft mice model. Asian Pac J Cancer Prev, 14, 409-12. https://doi.org/10.7314/APJCP.2013.14.1.409
  36. Yu Q, Geng Y, Sicinski P (2001). Specific protection against breast cancers by cyclin D1 ablation. Nat, 411, 1017-21. https://doi.org/10.1038/35082500
  37. Zeybek U, Yaylim I, Ozkan NE, et al (2013). Cyclin D1 gene G870A variants and primary brain tumors. APJCP, 14, 4101-6.
  38. Zhang T, Chen X, Qu L, et al (2004). Chrysin and its phosphate ester inhibit cell proliferation and induce apoptosis in Hela cells. Bioorgan Med Chem, 12, 6097-105. https://doi.org/10.1016/j.bmc.2004.09.013
  39. Zhang YY, Xu ZN, Wang JX, et al (2012). G1/S-specific cyclin-D1 might be a prognostic biomarker for patients with laryngeal squamous cell carcinoma. Asian Pac J Cancer Prev, 13, 2133-7. https://doi.org/10.7314/APJCP.2012.13.5.2133
  40. Zhou QM, Wang XF, Liu XJ, et al (2011). Curcumin enhanced antiproliferative effect of mitomycin C in human breast cancer MCF-7 cells in vitro and in vivo. Acta Pharmacol Sinica, 32, 1402-10. https://doi.org/10.1038/aps.2011.97

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